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Arai, Yoichi; Hasegawa, Kenta; Watanabe, So; Watanabe, Masayuki; Minowa, Kazuki*; Matsuura, Haruaki*; Hagura, Naoto*; Katsuki, Kenta*; Arai, Tsuyoshi*; Konishi, Yasuhiro*
Journal of Radioanalytical and Nuclear Chemistry, 9 Pages, 2023/00
Times Cited Count:0 Percentile:0.01(Chemistry, Analytical)Saga, Ryo*; Matsuya, Yusuke; Sato, Hikari*; Hasegawa, Kazuki*; Obara, Hideki*; Komai, Fumio*; Yoshino, Hironori*; Aoki, Masahiko*; Hosokawa, Yoichiro*
Radiotherapy and Oncology, p.109444_1 - 109444_9, 2023/00
Times Cited Count:2 Percentile:0(Oncology)When treating non-small cell lung cancer (NSCLC), stereotactic body radiotherapy (SBRT) with high-dose irradiation is often utilized. The fractionation schemes and curative effects can be evaluated by mathematical models for predicting cell survival curve. Such model parameters can be determined from in vitro experiment, but they are empirically determined based on experiences in clinics. As such, there is a large gap between in vitro and clinical study. As such background, translational study between in vitro cell survival and clinical curative effects is necessary. In this study, explicitly considering existence of cancer stem-like cells (CSCs), we developed an all-in-one model for predicting both in vitro cell survival and clinical curative effects (integrated microdosimetric-kinetic (IMK) model) and performed retrospective evaluation of clinical outcomes following SBRT for NSCLC in Hirosaki University Hospital. As a result, the IMK model successfully reproduced both in vitro cell survival and the tumor control probability with various fractionation schemes (i.e., 6-10 Gy per fraction). The developed model would contribute on precisely understanding the impact of CSCs on curative effects after SBRT for NSCLC with high precision.
Saga, Ryo*; Matsuya, Yusuke; Takahashi, Rei*; Hasegawa, Kazuki*; Date, Hiroyuki*; Hosokawa, Yoichiro*
Scientific Reports (Internet), 11(1), p.8258_1 - 8258_10, 2021/04
Times Cited Count:4 Percentile:47.6(Multidisciplinary Sciences)Hyaluronan synthesis inhibitor 4-methylumbelliferone (4-MU) is a candidate of radiosensitizers in X-ray therapy. The curative effects under such 4-MU administration have been investigated in vitro; however, the radiosensitizing mechanisms remain unclear. Here, we investigated the radiosensitizing effects under 4-MU treatment from cell experiments and model estimations. We generated experimental surviving fractions of human fibrosarcoma cells (HT1080) after 4-MU treatment combined with X-ray irradiation. Meanwhilst, we also modelled the pharmacological effects of 4-MU treatment and theoretically analyzed the synergetic effects between 4-MU treatment and X-ray irradiation. The results show that the enhancement of cell killing by 4-MU treatment is the greatest in the intermediate dose range of around 4 Gy, which indicates the involvement of intercellular communication. In addition, the oxidative stress level, which leads to DNA damage induction, significantly increased under 4-MU treatment, and the radiosensitization by 4-MU can be suppressed by the inhibitors for intercellular communication. These findings suggest that the synergetic effects between 4-MU treatment and irradiation are predominantly attributed to intercellular communication and provide more efficient tumour control than conventional X-ray therapy.
Yang, Z. H.*; Kubota, Yuki*; Corsi, A.*; Yoshida, Kazuki; Sun, X.-X.*; Li, J. G.*; Kimura, Masaaki*; Michel, N.*; Ogata, Kazuyuki*; Yuan, C. X.*; et al.
Physical Review Letters, 126(8), p.082501_1 - 082501_8, 2021/02
Times Cited Count:43 Percentile:96.7(Physics, Multidisciplinary)A quasifree (,) experiment was performed to study the structure of the Borromean nucleus B, which had long been considered to have a neutron halo. By analyzing the momentum distributions and exclusive cross sections, we obtained the spectroscopic factors for and orbitals, and a surprisingly small percentage of 9(2)% was determined for . Our finding of such a small component and the halo features reported in prior experiments can be explained by the deformed relativistic Hartree-Bogoliubov theory in continuum, revealing a definite but not dominant neutron halo in B. The present work gives the smallest - or -orbital component among known nuclei exhibiting halo features and implies that the dominant occupation of or orbitals is not a prerequisite for the occurrence of a neutron halo.
Iida, Kazuki*; Kofu, Maiko; Suzuki, Katsuhiro*; Murai, Naoki; Kawamura, Seiko; Kajimoto, Ryoichi; Inamura, Yasuhiro; Ishikado, Motoyuki*; Hasegawa, Shunsuke*; Masuda, Takatsugu*; et al.
Journal of the Physical Society of Japan, 89(5), p.053702_1 - 053702_5, 2020/05
Times Cited Count:19 Percentile:81.08(Physics, Multidisciplinary)Saga, Ryo*; Matsuya, Yusuke; Takahashi, Rei*; Hasegawa, Kazuki*; Date, Hiroyuki*; Hosokawa, Yoichiro*
Journal of Radiation Research, 60(3), p.298 - 307, 2019/05
Times Cited Count:23 Percentile:81.57(Biology)In radiotherapy, it is recognized that cancer stem cells (CSCs) in tumor tissue shows radio-resistance. However, the relationship between content percentage of the CSCs and dose-response curve on cell survival remain unclear. In this study, we developed a stochastic model considering progeny cells and stem cells, and investigated the impact of stem cells on radio-sensitivity. From the flow-cytometric analysis (cell experiments), the content percentage of stem cells was 3.2% or less which agreed well with the model estimation from the cell survival curve. Based on the verification, it is suggested that cell survival in high-dose range is largely affected by the CSCs. In addition, regarding the sub-population of stem cells, the present model well reproduces the dose response on lethal lesions to DNA comparing with the conventional LQ model. This outcome indicates that the stem cells must be considered for describing the dose-response curve in wide dose range.
Ekawa, Hiroyuki; Ashikaga, Sakiko; Hasegawa, Shoichi; Hashimoto, Tadashi; Hayakawa, Shuhei; Hosomi, Kenji; Ichikawa, Yudai; Imai, Kenichi; Kimbara, Shinji*; Nanamura, Takuya; et al.
Progress of Theoretical and Experimental Physics (Internet), 2019(2), p.021D02_1 - 021D02_11, 2019/02
Times Cited Count:25 Percentile:83.76(Physics, Multidisciplinary)Yamana, Hajimu*; Moriyama, Hirotake*; Asano, Hideki*; Shiokawa, Yoshinobu*; Yamamura, Tomoo*; Hasegawa, Kazuki*; Kimura, Akihiro*; Umekita, Satoshi*
JAERI-Tech 2002-018, 33 Pages, 2002/03
no abstracts in English
Kato, Toshihiro; Iwatsuki, Teruki; Munemoto, Takashi; Kubota, Mitsuru; Hayashida, Kazuki; Nakata, Kotaro*; Hasegawa, Takuma*
no journal, ,
no abstracts in English
Hasegawa, Takayuki*; Uemura, Masaharu*; Takueuchi, Kazuki*; Kodaka, Takuya*; Fukada, Noboru*; Umesaki, Norimasa*; Fukushima, Sei*; Teraoka, Yuden; Haruyama, Yuichi*; Niibe, Masahito*; et al.
no journal, ,
no abstracts in English
Hasegawa, Takuma*; Nakata, Kotaro*; Tomioka, Yuichi*; Ota, Tomoko*; Hama, Katsuhiro; Iwatsuki, Teruki; Kato, Toshihiro*; Hayashida, Kazuki
no journal, ,
Groundwater dating using C, He, noble gas method was conducted in Mizunami Underground Research laboratory. The availability of degassing method for , flux estimation method for He were examined. It was identified that the groundwater have been derived in glacial age. The integration of distinct dating method is available to estimate precise age of groundwater.
Hasegawa, Takuma*; Nakata, Kotaro*; Tomioka, Yuichi*; Ota, Tomoko*; Hama, Katsuhiro; Iwatsuki, Teruki; Kato, Toshihiro*; Hayashida, Kazuki
no journal, ,
no abstracts in English
Hasegawa, Takuma*; Nakata, Kotaro*; Tomioka, Yuichi*; Ota, Tomoko*; Hama, Katsuhiro; Iwatsuki, Teruki; Kato, Toshihiro*; Hayashida, Kazuki
no journal, ,
Groundwater dating was conducted to Mizunami Underground Research Laboratory (MIU). C age and He age were estimated about 20,000 years around MIU. The estimation of noble gas temperature is also conducted. The estimated recharge temperature is around 6C. This temperature is about 9C cooler than present annual average temperature, which agree with estimation of modern analog method conducted near MIU.
Takahashi, Rei*; Saga, Ryo*; Matsuya, Yusuke; Hasegawa, Kazuki*; Fukui, Roman*; Hosokawa, Yoichiro*
no journal, ,
Inflammatory responses after irradiation induce antioxidant activity, leading to radioresistance in cancer cells. In our previous study, it was found that a hyaluronan synthesis inhibitor (4-MU) can suppress inflammatory responses, however the underlying mechanisms are still unknown. In this study, we aimed to clarify the radiosensitization mechanism induced by 4-MU in the application of cell-killing models. In terms of in vitro experiments, cultured human fibrosarcoma cells were exposed to X-rays in presence of 100 M 4-MU and scavenger of intercellular signalling (1% DMSO, 40 M c-PTIO), and we measured the relation between dose and cell viability by means of colony assay. The experimental results were also interpreted by using integrated cell-killing model which has been developed so as to consider non-targeted effects. From the experiments, it was found that a combination of 4-MU treatment and irradiation significantly reduced the cell survival. This reduced viability was suppressed in the presence of DMSO or c-TPIO. Meanwhile, the model analysis showed such an increase of cell-killing is predominantly attributed by non-targeted effects. These results suggest that the radiosensetization promoted by 4-MU treatment is predominantly attributed to the accumulation in non-targeted effects.
Yoshikawa, Masashi*; Minowa, Kazuki*; Matsuura, Haruaki*; Hasegawa, Kenta; Arai, Yoichi; Watanabe, So; Konishi, Yasuhiro*
no journal, ,
no abstracts in English