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Watanabe, Ritsuko*; Kai, Takeshi; Hattori, Yuya*
Radioisotopes, 66(11), p.525 - 530, 2017/11
To understand the mechanisms of radiation biological effects, modeling and simulation studies are important. In particular, simulation approach is powerful tool to evaluate modeling of mechanisms and the relationship among experimental results in different spatial scale of biological systems such as DNA molecular and cell. This article summarizes our approach to evaluate radiation action on DNA and cells by combination of knowledge in radiation physics, chemistry and biology. It contains newly theoretical approach to estimate physico-chemical process of DNA damage induction in addition to typical method of DNA damage prediction. Outline of the mathematical model for dynamics of DNA damage and cellular response is also presented.
CsWatanabe, Ritsuko; Hattori, Yuya; Kai, Takeshi
International Journal of Radiation Biology, 92(11), p.660 - 664, 2016/11
Times Cited Count：3 Percentile：26.09(Biology)To understand the effect of internal exposure of Cs, we focus on estimation of microscopic energy deposition pattern and DNA damage induced by directly emitted electrons (beta-rays, internal conversion electrons, Auger electrons) from Cs. Monte Carlo track simulation method was used to calculate the microscopic energy deposition pattern. To simulate the energy deposition by directly emitted electrons, we considered the multiple ejections of electrons after internal conversion. Induction process of DNA strand breaks and base lesions was modeled and simulated using Monte Carlo methods for cell mimetic condition. The yield and spatial distribution of simple and complex DNA damage were calculated for the cases of 
-rays and electrons from Cs. The simulation showed that significant difference of DNA damage spectrum was not caused by the difference between secondary electron spectrum by -rays and directly ejected electron spectrum. The result support that the existing evaluation that internal exposure and external exposure are almost equivalent.
Hattori, Yuya; Yokoya, Akinari; Watanabe, Ritsuko
BMC Systems Biology (Internet), 9, p.90_1 - 90_22, 2015/12
Times Cited Count：17 Percentile：64.01(Mathematical & Computational Biology)The radiation-induced bystander effect is a biological response observed in non-irradiated cells surrounding an irradiated cell, which is known to be caused by two intercellular signaling pathways. However, the behavior of the signals is largely unknown. To investigate the role of these signaling pathways, we developed a mathematical model to describe the cellular response to direct irradiation and the bystander effect, with a particular focus on cell-cycle modification. The analysis of model dynamics revealed that bystander effect on cell cycle modification was different between low-dose irradiation and high-dose irradiation. We demonstrated that signaling through both pathways induced cell cycle modification via the bystander effect. By simulating various special and temporal conditions of irradiation and cell characteristics, our model will be a powerful tool for the analysis of the bystander effect.
Hattori, Yuya; Suzuki, Michiyo; Funayama, Tomoo; Kobayashi, Yasuhiko; Yokoya, Akinari; Watanabe, Ritsuko
Radiation Protection Dosimetry, 166(1-4), p.142 - 147, 2015/09
Times Cited Count：5 Percentile：37.04(Environmental Sciences)Cell-to-cell communication is one of the important factors to understand the mechanisms of radiation-induced responses such as radiation-induced bystander effects at low doses. In the present study, we propose simulation-based analyses of the intercellular signal transmissions between the individual cells in the cellular population. We developed the transmissions of two types of signals, i.e., X is transmitted via culture medium and Y is transmitted via gap junctions based on the diffusion equation. To observe the cell cycle as the response of cell induced by the signals, X and Y, we represented the cell cycle as a virtual clock including several check-point pathways and the cyclic process (G1, S, G2, M phases). The cellular population was divided into the grids (cells), and the signals and the clock were calculated for each grid. The signals, X, Y, were transmitted to the cells and stopped the clocks at the check points. Furthermore, the radiation was modeled as the radiation signal, Z, which affected the clock and the signals, X and Y. We input the radiation signal, Z, to specific cells, and simulated the behaviors of the clock of each cell and signals, X and Y. We will discuss the usefulness of our model for investigating the mechanisms of radiation-induced responses of the cell cycle via cell-to-cell communications.
locus in normal human fibroblasts induced by C-, Ne- and Ar-ion microbeamsSuzuki, Masao*; Funayama, Tomoo; Yokota, Yuichiro; Muto, Yasuko*; Suzuki, Michiyo; Ikeda, Hiroko; Hattori, Yuya; Kobayashi, Yasuhiko
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 78, 2015/03
We have been studying the radiation-quality dependent bystander cellular effects, such as cell killing, mutation induction and chromosomal damage, using heavy-ion microbeams with different ion species. This year we focused on the ion-species dependent bystander mutagenic effect on locus in normal human fibroblasts. The confluent culture were irradiated using a 256 (16
16)-cross-stripe method using C, Ne and Ar microbeam. Gene mutation on locus was detected with 6-thioguanine resistant clones. The mutation frequency in cells irradiated with C-ion microbeams was 6 times higher than that of non-irradiated control cells and of the sample treated with specific inhibitor of gap-junction cell-to-cell communication. On the other hand, no enhanced mutation frequencies were observed in cells irradiated with either Ne- or Ar-ion microbeams. There is clear evidence that the bystander mutagenic effect via gap-junction communication depends on radiation quality.
Suzuki, Michiyo; Hattori, Yuya; Sakashita, Tetsuya; Funayama, Tomoo; Yokota, Yuichiro; Ikeda, Hiroko; Kobayashi, Yasuhiko
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 88, 2015/03
mutants to microbeam irradiationSakashita, Tetsuya; Suzuki, Michiyo; Hattori, Yuya; Ikeda, Hiroko; Muto, Yasuko*; Yokota, Yuichiro; Funayama, Tomoo; Hamada, Nobuyuki*; Shirai, Kana*; Kobayashi, Yasuhiko
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 74, 2015/03
An increasing body of data indicates that ionizing radiation affects the nervous system and alters its function. Recently, we reported that chemotaxis of during the salt chemotaxis learning (SCL), that is conditioned taste aversion to NaCl, was modulated by carbon ion irradiation, i.e. accelerated decrease in chemotaxis to NaCl during the SCL. However, we had no direct evidence for the interaction of ionizing radiation with the central neuronal tissue (nerve ring) in . Microbeam irradiation is useful to analyze direct radiation effects at a cellular or tissue level. Thus, we applied the microbeam irradiation of the nerve ring and examined the effect on the SCL.
Suzuki, Masao*; Autsavapromporn, N.*; Usami, Noriko*; Funayama, Tomoo; Plante, I.*; Yokota, Yuichiro; Muto, Yasuko*; Suzuki, Michiyo; Ikeda, Hiroko; Hattori, Yuya; et al.
Journal of Radiation Research, 55(Suppl.1), P. i54, 2014/03
based on FitzHugh-Nagumo equationsHattori, Yuya; Suzuki, Michiyo; Soh, Zu*; Kobayashi, Yasuhiko; Tsuji, Toshio*
Artificial Life and Robotics, 17(2), p.173 - 179, 2012/12
Hattori, Yuya; Suzuki, Michiyo; Soh, Zu*; Kobayashi, Yasuhiko; Tsuji, Toshio*
Neural Computation, 24(3), p.635 - 675, 2012/03
Times Cited Count：6 Percentile：33.04(Computer Science, Artificial Intelligence)Hattori, Yuya; Suzuki, Michiyo; So, Zu*; Kobayashi, Yasuhiko; Tsuji, Toshio*
Proceedings of 17th International Symposium on Artificial Life and Robotics (AROB 2012) (CD-ROM), p.690 - 695, 2012/01
Hattori, Yuya; Suzuki, Michiyo; Soh, Zu*; Kobayashi, Yasuhiko; Tsuji, Toshio*
Lecture Notes in Computer Science 6352, p.401 - 410, 2010/09
Suzuki, Michiyo; Hattori, Yuya; Sakashita, Tetsuya; Funayama, Tomoo; Yokota, Yuichiro; Ikeda, Hiroko; Kobayashi, Yasuhiko
no journal, ,
no abstracts in English
Suzuki, Michiyo; Hattori, Yuya; Sakashita, Tetsuya; Funayama, Tomoo; Yokota, Yuichiro; Muto, Yasuko; Ikeda, Hiroko; Kobayashi, Yasuhiko
no journal, ,
no abstracts in English
using heavy-ion microbeamSakashita, Tetsuya; Suzuki, Michiyo; Muto, Yasuko*; Hattori, Yuya; Ikeda, Hiroko; Yokota, Yuichiro; Funayama, Tomoo; Hamada, Nobuyuki*; Fukamoto, Kana*; Kobayashi, Yasuhiko
no journal, ,
An increasing body of data indicates that ionizing radiation causes learning impairments. To understand the effects of ionizing radiation on the nervous system, we have studied the salt chemotaxis learning in 
. We recently found the modulatory effect of -rays on the salt chemotaxis learning that was manifested as a decrease in chemotaxis. However, we have no direct evidence for the interaction of ionizing radiation with the central neuronal tissue (nerve ring) of the nervous system in . Localized ionizing irradiation is useful to analyze radiation effects at a cellular or tissue level. Thus, to investigate the effects on the nerve ring, we used the heavy-ion microbeam system installed at the Takasaki Ion accelerators for Advanced Radiation Application of JAEA. In this presentation, we will discuss the preliminary results and a future vision of this study.
Hattori, Yuya; Suzuki, Michiyo; Kobayashi, Yasuhiko; Tsuji, Toshio*
no journal, ,
Kobayashi, Yasuhiko; Funayama, Tomoo; Taguchi, Mitsumasa; Tanaka, Atsushi; Wada, Seiichi*; Watanabe, Hiroshi*; Furusawa, Yoshiya*; Kiguchi, Kenji*; Fukamoto, Kana*; Sakashita, Tetsuya; et al.
no journal, ,
The application of localized radiation using heavy-ion microbeams eliminates the effect of non-uniform ion hits on cell population, since individual cells can be irradiated one by one with a defined number of energetic heavy ions. Another advantage associated with the use of heavy-ion microbeam irradiation concerns the precise detection of ion-hit position on micron-scale targets to obtain the information on the position of ion traversal and on cellular responses induced by ion hit simultaneously. Therefore microbeam is an operative means to elucidate initial cellular responses together with the relationship with ion track structure. The use of heavy-ion microbeams has not been restricted to the area of radiation biology. Targeted irradiation using heavy-ion microbeams has been applied to various biological studies, such as plant physiology or developmental biology, as a radio-microsurgical tool to inactivate specific tissue or cell populations in multicellular organisms and to investigate their function. The outlines of these studies, which were carried out using our collimated heavy-ion microbeam at JAEA-Takasaki, will be introduced.
-ray irradiation on locomotory behavior and mechanosensation in Suzuki, Michiyo; Sakashita, Tetsuya; Kikuchi, Masahiro; Funayama, Tomoo; Yokota, Yuichiro; Hattori, Yuya; Kobayashi, Yasuhiko
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; A Computational approach for understanding radiation effectsHattori, Yuya; Suzuki, Michiyo; Tsuji, Toshio*; Kobayashi, Yasuhiko
no journal, ,
no abstracts in English
Soh, Zu*; Tsuji, Toshio*; Suzuki, Michiyo; Hattori, Yuya; Takiguchi, Noboru*; Otake, Hisao*
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