Isolation of a novel UVB-tolerant rice mutant obtained by exposure to carbon-ion beams

Takano, Nao*; Takahashi, Yuko*; Yamamoto, Mitsuru*; Teranishi, Mika*; Yamaguchi, Hiroko*; Sakamoto, Ayako; Hase, Yoshihiro; Fujisawa, Hiroko*; Wu, J.*; Matsumoto, Takashi*; et al.

Journal of Radiation Research, 54(4), p.637 - 648, 2013/07

https://doi.org/10.1093/jrr/rrt007

Analysis of mutated region on DNA in ion beam-induced UVB tolerant or sensitive rice mutant

Takano, Nao*; Takahashi, Yuko*; Yamamoto, Mitsuru*; Teranishi, Mika*; Hase, Yoshihiro; Sakamoto, Ayako; Tanaka, Atsushi; Hidema, Jun*

JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 104, 2012/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2011-043.pdf

Characteristics of carbon ion beam induced UVB tolerant and sensitive mutants of rice

Takano, Nao*; Takahashi, Yuko*; Yamamoto, Mitsuru*; Teranishi, Mika*; Hase, Yoshihiro; Sakamoto, Ayako; Tanaka, Atsushi; Hidema, Jun*

no journal, , 

To develop gene resources related to UVB tolerance of rice, UVB resistant rice cultivar Sasanishiki was irradiated with carbon ion beams and successfully isolated two mutant lines: UVB resistant UVTSa-319 and UVB sensitive UVSSa-1. These mutants have similar levels of the amount of UV-absorbing compounds and the repair activities of cyclobutane pyrimidine dimers and (6-4) photoproducts which have been thought as factors related to UVB tolerance in Sasanishiki. We performed Array CGH to estimate mutated region and found that an approximate 40 kbp region on chromosome 7 containing 2 unknown genes was deleted in UVTSa-319. Also, the mutant UVSSa-1 had a deletion on chromosome 7 covering 2 other genes. Next, to deduce the relationships between deleted genes and UVB tolerant mechanism in these mutants, microarray analyses were performed using RNAs of Sasanishiki and these mutants. We discuss the functions of deleted genes and relationships of UVB tolerance or sensitivity.

Analysis of DNA damages induced by ion beam, ray and UVB radiation in Arabidopsis

Teranishi, Mika*; Yamaguchi, Hiroko*; Sakamoto, Ayako; Hidema, Jun*

no journal, , 

Analysis of the ion-beam induced UVB resistant rice

Takano, Nao*; Takahashi, Yuko*; Yamamoto, Mitsuru*; Teranishi, Mika*; Hase, Yoshihiro; Sakamoto, Ayako; Tanaka, Atsushi; Hidema, Jun*

no journal, , 

no abstracts in English

Analysis of DNA strand breaks induced by carbon ion beams in Arabidopsis

Teranishi, Mika*; Yamaguchi, Hiroko*; Sakamoto, Ayako; Hidema, Jun*

no journal, , 

no abstracts in English

Effect of oxidative DNA damage on radiation-induced mutagenesis in

Yoshihara, Ryohei; Hase, Yoshihiro; Nozawa, Shigeki; Narumi, Issei; Takimoto, Koichi*; Hidema, Jun*; Sakamoto, Ayako

no journal, , 

We analyzed -ray-induced mutation spectrum in Arabidopsis seedling to investigate the effect of oxidative DNA damage on mutagenesis. Moreover, to evaluate the effect of nucleotide pool sanitization system on -ray-induced mutations, we analyzed a mutant deficient in the Arabidopsis gene that is a homologue of . The -ray sensitivity of the mutant was slightly higher, but not statistically significant, than that of wild type plant. However, the mutation spectra induced by -rays were different between the wild type and plants. From these results, it was suggested that the deficiency obviously affects on the mutagenesis but not the plant growth following -irradiation.

Characteristics of carbon ion beam induced UVB hyper-resistant mutant of rice

Takano, Nao*; Takahashi, Yuko*; Yamamoto, Mitsuru*; Teranishi, Mika*; Yokozawa, Daisuke*; Hase, Yoshihiro; Sakamoto, Ayako; Tanaka, Atsushi; Hidema, Jun*

no journal, , 

no abstracts in English