Revealing an origin of temperature-dependent structural change in intrinsically disordered proteins

Inoue, Rintaro*; Oda, Takashi; Nakagawa, Hiroshi; Tominaga, Taiki*; Ikegami, Takahisa*; Konuma, Tsuyoshi*; Iwase, Hiroki*; Kawakita, Yukinobu; Sato, Mamoru*; Sugiyama, Masaaki*

Biophysical Journal, 124(3), p.540 - 548, 2025/02

https://doi.org/10.1016/j.bpj.2024.12.022

Interlaboratory comparison of electron paramagnetic resonance tooth enamel dosimetry with investigations of the dose responses of the standard samples

Toyoda, Shin*; Inoue, Kazuhiko*; Yamaguchi, Ichiro*; Hoshi, Masaharu*; Hirota, Seiko*; Oka, Toshitaka; Shimazaki, Tatsuya*; Mizuno, Hideyuki*; Tani, Atsushi*; Yasuda, Hiroshi*; et al.

Radiation Protection Dosimetry, 199(14), p.1557 - 1564, 2023/09

https://doi.org/10.1093/rpd/ncad150

Interlaboratory comparison studies are important for radiation dosimetry in order to demonstrate how the technique is universally available. The set of standard samples are examined in each participating laboratory in the present study. After a set of standard samples together with the samples with unknown doses, which were prepared in the same laboratory as the standard samples, are measured at a participating laboratory, those samples are sent to another participating laboratory for next measurement. There is some small difference observed in the sensitivity (the slope of the dose response line) of the standard samples while the differences in the obtained doses for the samples with unknown doses are rather systematic, implying that the difference is mostly due to the samples but not to measurements.

None

Nagata, Shuhei*; Ogawa, Yusuke*; Suzuki, Satoru*; Inoue, Hiroyuki*; Watanabe, Yutaka*; Yamamoto, Masahiro*; Abe, Hiroshi*; Mitsui, Seiichiro

NUMO-TR-22-02, p.21 - 22, 2023/03

https://www.numo.or.jp/technology/technical_report/NUMO-TR-22-02.pdf

no abstracts in English

Sodium-cooled Fast Reactors

Ohshima, Hiroyuki; Morishita, Masaki*; Aizawa, Kosuke; Ando, Masanori; Ashida, Takashi; Chikazawa, Yoshitaka; Doda, Norihiro; Enuma, Yasuhiro; Ezure, Toshiki; Fukano, Yoshitaka; et al.

Sodium-cooled Fast Reactors; JSME Series in Thermal and Nuclear Power Generation, Vol.3, 631 Pages, 2022/07

https://shop.elsevier.com/books/sodium-cooled-fast-reactors/morishita/978-0-12-824076-2

This book is a collection of the past experience of design, construction, and operation of two reactors, the latest knowledge and technology for SFR designs, and the future prospects of SFR development in Japan. It is intended to provide the perspective and the relevant knowledge to enable readers to become more familiar with SFR technology.

Conformational dynamics of a multidomain protein by neutron scattering and computational analysis

Nakagawa, Hiroshi; Saio, Tomohide*; Nagao, Michihiro*; Inoue, Rintaro*; Sugiyama, Masaaki*; Ajito, Satoshi; Tominaga, Taiki*; Kawakita, Yukinobu

Biophysical Journal, 120(16), p.3341 - 3354, 2021/08

https://doi.org/10.1016/j.bpj.2021.07.001

A multi-domain protein can have various conformations in solution. Interactions with other molecules result in the stabilization of one of the conformations and change in the domain dynamics. SAXS, a well-established experimental technique, can be employed to elucidate the conformation of a multi-domain protein in solution. NSE spectroscopy is a promising technique for recording the domain dynamics in nanosecond and nanometer scale. Despite the great efforts, there are still under development. Thus, we quantitatively removed the contribution of diffusion dynamics and hydrodynamic interactions from the NSE data via incoherent scattering, revealing the differences in the domain dynamics of the three functional states of a multi-domain protein, MurD. The differences among the three states can be explained by two domain modes.

Dynamics of proteins with different molecular structures under solution condition

Inoue, Rintaro*; Oda, Takashi*; Nakagawa, Hiroshi; Tominaga, Taiki*; Saio, Tomohide*; Kawakita, Yukinobu; Shimizu, Masahiro*; Okuda, Aya*; Morishima, Ken*; Sato, Nobuhiro*; et al.

Scientific Reports (Internet), 10, p.21678_1 - 21678_10, 2020/12

https://doi.org/10.1038/s41598-020-78311-4

Incoherent quasielastic neutron scattering (iQENS) is a fascinating technique for investigating the internal dynamics of protein. However, both low flux of neutron beam and absence of analytical procedure for extracting the internal dynamics from iQENS profile have been obstacles for studying it under physiological condition (in solution). Thanks to the recent development of neutron source, spectrometer and computational technique, they enable us to decouple internal dynamics, translational and rotational diffusions from the iQENS profile. The internal dynamics of two proteins: globular domain protein (GDP) and intrinsically disordered protein (IDP) in solution were studied. It was found that the average relaxation rate of IDP was larger than that of GDP. Through the detailed analyses on their internal dynamics, it was revealed that the fraction of mobile H atoms in IDP was much higher than that in GDP. Interestingly, the fraction of mobile H atoms was closely related to the fraction of H atoms on highly solvent exposed surfaces. The iQENS study presented that the internal dynamics were governed by the highly solvent exposed amino acid residues depending upon protein molecular architectures.

Corrosion monitoring in humidity-controlled environment simulating gamma ray irradiation

Omori, Atsushi*; Akiyama, Eiji*; Abe, Hiroshi*; Hata, Kuniki; Sato, Tomonori; Kaji, Yoshiyuki; Inoue, Hiroyuki*; Taguchi, Mitsumasa*; Seito, Hajime*; Tada, Eiji*; et al.

Zairyo To Kankyo, 69(4), p.107 - 111, 2020/04

https://doi.org/10.3323/jcorr.69.107

To evaluate the effect of oxidants, which are formed by radiolysis of water under gamma ray irradiation, on the corrosion of a carbon steel in humid environment, ozone was introduced as a model oxidant in to humidity-controlled air at 50C in a thermo-hygrostat chamber. Corrosion monitoring was performed by using an Atmospheric Corrosion Monitor-type (ACM) sensor consisting of a carbon steel anode and an Ag cathode. The output current of the ACM sensor was increased with the increase in relative humidity and it was obviously increased with the increase in the introduced ozone concentration at each relative humidity. The results indicate that ozone accelerates the corrosion of the carbon steel. The effect of ozone on the corrosion acceleration is attributed to the fast reduction reaction and fast dissolution reaction in to water compared to that of oxygen.

Ultra-high temperature creep rupture and transient burst strength of ODS steel claddings

Yano, Yasuhide; Sekio, Yoshihiro; Tanno, Takashi; Kato, Shoichi; Inoue, Toshihiko; Oka, Hiroshi; Otsuka, Satoshi; Furukawa, Tomohiro; Uwaba, Tomoyuki; Kaito, Takeji; et al.

Journal of Nuclear Materials, 516, p.347 - 353, 2019/04

https://doi.org/10.1016/j.jnucmat.2019.01.052

9Cr-ODS steel claddings consisting of tempered martensitic matrix, showed prominent creep rupture strength at 1000 C, which surpassed that of heat-resistant austenitic steels although creep rupture strength of tempered martensitic steels is generally lower than that of austenitic steels at high temperatures. The measured creep rupture strength of 9Cr-ODS steel claddings at 1000 C was higher than that from extrapolated creep rupture trend curves formulated using data at temperatures from 650 to 850 C. This superior strength seemed to be owing to transformation of the matrix from the -phase to the -phase. The transient burst strengths for 9Cr-ODS steel were much higher than those for 11Cr-ferritic/martensitic steel (PNC-FMS). Cumulative damage fraction analyses suggested that the life fraction rule can be used for the rupture life prediction of 9Cr-ODS steel and PNC-FMS claddings in the transient and accidental events with a certain accuracy.

Neutron biology for next generation; Report from J-PARC workshop "Neutron Biology for Next Generation"; March 22nd-23rd, 2017, Ibaraki Quantum Beam Research Center

Sugiyama, Masaaki*; Nakagawa, Hiroshi; Inoue, Rintaro*; Kawakita, Yukinobu

JAEA-Review 2017-024, 40 Pages, 2017/12

JAEA-Review-2017-024.pdf:8.69MB

Now-a-days, promotion of life science by utilizing neutron (neutrons biology) is highly demanded in our country, following installation and improvement of high quality and intensity neutron sources at J-PARC and JRR-3. Aiming at accelerating development of neutrons biology in our country, an international workshop "Neutron biology for next generation" was held as a J-PARC Workshop at Ibaraki Quantum Beam Research Center from 22 March to 23 March in 2017. In the workshop, latest instruments, new-fashioned methodologies, recent scientific results and future perspectives were extensively discussed by domestic neutron instrumental scientists and domestic/foreign neutron biologists. This is a report of the workshop summarized by organizers.

Ultra-high temperature tensile properties of ODS steel claddings under severe accident conditions

Yano, Yasuhide; Tanno, Takashi; Oka, Hiroshi; Otsuka, Satoshi; Inoue, Toshihiko; Kato, Shoichi; Furukawa, Tomohiro; Uwaba, Tomoyuki; Kaito, Takeji; Ukai, Shigeharu*; et al.

Journal of Nuclear Materials, 487, p.229 - 237, 2017/04

https://doi.org/10.1016/j.jnucmat.2017.02.021

Ultra-high temperature ring tensile tests were carried out to investigate the tensile behavior of oxide dispersion strengthened (ODS) steel claddings and wrapper materials under severe accident conditions; temperatures ranged from room temperature to 1400C which is near the melting point of core materials. The experimental results showed that tensile strength of 9Cr-ODS steel claddings was highest in the core materials at the ultra-high temperatures between 900 and 1200C, but that there was significant degradation in tensile strength of 9Cr-ODS steel claddings above 1200C. This degradation was attributed to grain boundary sliding deformation with / transformation, which was associated with reduced ductility. On the other hand, tensile strength of recrystallized 12Cr-ODS and FeCrAl-ODS steel claddings retained its high value above 1200 C unlike the other tested materials. Present study includes the result of "R&D of ODS ferritic steel fuel cladding for maintaining fuel integrity at the high temperature accident condition" entrusted to Hokkaido University by the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT).