Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Katsuyama, Chie*; Nashimoto, Hiroaki*; Nagaosa, Kazuyo*; Ishibashi, Tomotaka*; Furuta, Kazuki*; Kinoshita, Takeshi*; Yoshikawa, Hideki; Aoki, Kazuhiro; Asano, Takahiro*; Sasaki, Yoshito; et al.
FEMS Microbiology Ecology, 86(3), p.532 - 543, 2013/12
Times Cited Count：14 Percentile：36.72(Microbiology)Anaerobic microbial activity has a major influence on the subsurface environment, and should be considered in subsurface activities including the construction of radioactive waste repositories. We investigated denitrification and methanogenesis in anoxic groundwater from 140 m depth in two boreholes, where the redox potential fluctuated. The average maximum potential denitrification rates, measured under anaerobic conditions in the two boreholes using an 
N tracer. Methanogenesis candidates were detected by 16S rRNA gene analysis. Although the stable isotope signatures suggested that some of the dissolved methane was of biogenic origin, no potential for methane production was evident during the incubations. The groundwater at 140 m depth did not contain oxygen, had an Eh ranging from -144 to 6.8 mV, and was found to be a potential field for denitrification.
-sheet-rich prion protein in scrapie-infected neuroblastoma cells with human IgG1 antibody specific for -form prion proteinKubota, Toshiya*; Hamazoe, Yuta*; Hashiguchi, Shuhei*; Ishibashi, Daisuke*; Akasaka, Kazuyuki*; Nishida, Noriyuki*; Katamine, Shigeru*; Sakaguchi, Suehiro*; Kuroki, Ryota; Nakashima, Toshihiro*; et al.
Journal of Biological Chemistry, 287(17), p.14023 - 14039, 2012/04
Times Cited Count：5 Percentile：11.64(Biochemistry & Molecular Biology)We prepared -sheet-rich recombinant full-length prion protein (-form PrP) (Jackson, G. S., Hosszu, L. L., Power, A., Hill, A. F., Kenney, J., Saibil, H., Craven, C. J., Waltho, J. P., Clarke, A. R., and Collinge, J. (1999) Science 283, 1935-1937). Using this -form PrP and a human single chain Fv-displaying phage library, we have established a human IgG1 antibody specific to -form but not 
-form PrP, PRB7 IgG. When prion-infected ScN2a cells were cultured with PRB7 IgG, they generated and accumulated PRB7-binding granules in the cytoplasm with time, consequently becoming apoptotic cells bearing very large PRB7-bound aggregates. The SAF32 antibody recognizing the N-terminal octarepeat region of full-length PrP stained distinct granules in these cells as determined by confocal laser microscopy observation. When the accumulation of proteinase K-resistant PrP was examined in prion-infected ScN2a cells cultured in the presence of PRB7 IgG or SAF32, it was strongly inhibited by SAF32 but not at all by PRB7 IgG. Thus, we demonstrated direct evidence of the generation and accumulation of -sheet-rich PrP in ScN2a cells de novo. These results suggest first that PRB7-bound PrP is not responsible for the accumulation of -form PrP aggregates, which are rather an end product resulting in the triggering of apoptotic cell death, and second that SAF32-bound PrP lacking the PRB7-recognizing -form may represent so-called PrPSc with prion propagation activity. PRB7 is the first human antibody specific to -form PrP and has become a powerful tool for the characterization of the biochemical nature of prion and its pathology.
Sugiyama, Koichi*; Go, Shintaro*; Tomimatsu, Taro*; Kai, Tamito*; Nagae, Daisuke*; Ishibashi, Yuichi*; Matsunaga, Sotaro*; Nagata, Yuto*; Nishibata, Hiroki*; Washiyama, Kohei*; et al.
no journal, ,
We have successfully performed in-beam gamma-ray spectroscopy using the isomer-scope technique to study excited-state structure of neutron-rich heavy-actinide nuclei. The neutron-rich heavy-actinide nuclei were produced in the multinucleon-transfer reactions with a 
Cm target and 
O projectiles accelerated with the JAEA tandem accelerator. Projectile-like scattered particles were detected with Si E-
E telescopes placed at the backward angle, and target-like scattered particles of isomers were caught by an annular aluminum plate placed at about 60-mm downstream from the target. Four Ge detectors and 4 LaBr detectors were placed at the periphery of the aluminum plate, and detected gamma rays from the isomers. Gamma rays emitted from the actinide isomers were successfully observed with a good sensitivity owing to the tungsten shield placed between the target and the detectors.
