Characterization of Xenopus laevis thrombocytic cells stimulated by thrombopoietin in liquid culture

田原 彩香*; 谷崎 祐太*; 奥井 武仁*; 目黒 瑞枝; 木下 紗也香*; 前川 峻*; 山内 志毅*; 下地 美也子*; 石田 貴子*; 永井 豊*; et al.

no journal, , 

Most vertebrates have nucleated thrombocytes instead of platelets or megakaryocytes. African clawed frog Xenopus laevis, produces such thrombocytes in liver and spleen. We made a recombinant X.laevis thrombopoietin-like polypeptide (xlTPO) and found it had colony-forming activity in semi-solid assays of liver and spleen. Further studies focused on characterizing the thrombocytes and thrombocytic progenitors were performed to confirm whether xlTPO is a functional ortholog of mammalian TPO.