Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Harada, Satoshi*; Ehara, Shigeru*; Ishii, Keizo*; Sato, Takahiro; Yamazaki, Hiromichi*; Matsuyama, Shigeo*; Kamiya, Tomihiro
JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 89, 2012/01
Endo, Kiyoshi*; Shibata, Yasushi*; Yamamoto, Tetsuya*; Nakai, Kei*; Matsumura, Akira*; Sato, Takahiro; Yokoyama, Akihito; Koka, Masashi; Okubo, Takeru; Yamazaki, Akiyoshi; et al.
JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 86, 2012/01
Sakurai, Eiko*; Yanai, Kazuhiko*; Ishii, Keizo*; Fujiki, Kota*; Hiraishi, Shingo*; Yamazaki, Hiromichi*; Matsuyama, Shigeo*; Okamura, Nobuyuki*; Yokoyama, Akihito; Kada, Wataru; et al.
JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 84, 2012/01
Iwata, Yoshihiro*; Ishii, Keizo*; Kamiya, Tomihiro; Sato, Takahiro
JAEA-Review 2011-043, JAEA Takasaki Annual Report 2010, P. 90, 2012/01
Sato, Takahiro; Ishii, Keizo*
JAEA-Review 2010-078, 45 Pages, 2011/03
JAEA-Review-2010-078.pdf:3.92MB
The joint research on "Application of micro-PIXE and imaging technology to life science" supported by the Inter-organizational Atomic Energy Research Program, had been performed for three years, from 2006FY to 2009FY. Aiming to apply in-air micro-PIXE analytical system to life science, the research was consisting of 7 collaborative themes related to beam engineering for micro-PIXE and applied technology of element mapping in biological/medical fields. The system, so-called micro-PIXE camera, to acquire spatial element mapping in living cells was originally developed by collaborative research between the JAEA and the department of engineering of Tohoku University. This review covers these research results.
Endo, Kiyoshi*; Shibata, Yasushi*; Yoshida, Fumiyo*; Nakai, Kei*; Yamamoto, Tetsuya*; Matsumura, Akira*; Ishii, Keizo*; Sakai, Takuro; Sato, Takahiro; Oikawa, Masakazu*; et al.
Proceedings of 11th World Congress on Neutron Capture Therapy (ISNCT-11) (CD-ROM), 2 Pages, 2004/10
Micro PIXE, which is installed in a single end accelerator in JAERI, was used for quantitative analysis of boron and gadolinium distribution in a cell level. The micro beam of 1 
m diameter is possible to observe the distribution. In the adjustment procedure of the sample, first is a fix of mylar film by using a glass ring and a bite ring of 2cm diameter. Next the 9L cells were scattered on the washed film, and is cultivated on 37
C in medium until they form the mono-layer. After the Gd-BOPTA was added, it incubates for the 24-72 hour on 37C. The film is washed in the THAM liquid, and is directly put on liquid nitrogen. A vacuum drying for 24 hours is conducted in order to fix a film on holder. It is important to uniformly fix the cell in distribution analysis in the cell using Micro PIXE. In recent result, it became possible that the distribution of P, S, Gd, etc. was analyzed. But we could not distinguish whether K and Gd exist in the cell or whether it exists around the cell. It was indicated that these elements was leaked by the reason of cell breaking or other on the cytoplasm.
Sato, Takahiro; Ishii, Keizo*; Kamiya, Tomihiro; Sakai, Takuro; Oikawa, Masakazu*; Arakawa, Kazuo; Matsuyama, Shigeo*; Yamazaki, Hiromichi*
Nuclear Instruments and Methods in Physics Research B, 210, p.113 - 116, 2003/09
Times Cited Count：1 Percentile：12.35(Instruments & Instrumentation)A new detection apparatus for both low energy X-rays and back-scattered protons of MeV energy and its measurement system were developed. The detection apparatus consists of a multi-element detector, pre-amplifiers with active reset system and so on. The detector elements are arranged in the shape of a pentagonal pyramid and fully cover the sample. On each one detection side, 9 detector elements are arranged. The entire detection system consists thus of 45 detector elements. The micro-beam irradiates the sample through the center of the pentagonal pyramid and X-rays emitted from the sample are detected in close to 
geometry. This novel detection setup has about 10 times the sensitivity of a conventional Micro-PIXE camera. The counting rate of back-scattered protons per detection element is small despite lack of a passive absorber. It appears possible that X-rays from the very small quantity of elements could also be detected. The advantage over a conventional Micro-PIXE camera includes simultaneous detection of back-scattered protons from N, C and O.
Harada, Satoshi*; Tamakawa, Yoshiharu*; Ishii, Keizo*; Tanaka, Akira*; Sato, Takahiro; Matsuyama, Shigeo*; Yamazaki, Hiromichi*; Kamiya, Tomihiro; Sakai, Takuro; Arakawa, Kazuo; et al.
Nuclear Instruments and Methods in Physics Research B, 189(1-4), p.437 - 442, 2002/04
Times Cited Count：1 Percentile：12.41(Instruments & Instrumentation)To study the interactions between the induction of radiation-induced apoptosis and trace elements kinetics, human leukemia cells were irradiated in vitro by 
Co 
rays, after which the cells were evaluated for the detection of apoptosis and trace element imaging was carried out. The frequency of apoptosis was obtained by microscopic assay using TUNEL staining. The trace element distribution in the cell was determined by micro-PIXE. In the early phase of apoptosis, the maximum level of Fe accumulation was observed in the cell stroma. In the mid to end phase, Fe accumulation was diminished, and instead, Ca accumulation increased and Zn decreased in the nucleus. There appear to be two steps for the development of apoptosis: (1) the signaling from cell stroma to nucleus by Fe or an Fe-containing enzyme; and (2) the degeneration of the nucleus by Ca-dependent enzyme, and release of Zn from digested nucleus. Those strong accumulations may be new markers for apoptosis.
Sakai, Takuro; Kamiya, Tomihiro; Oikawa, Masakazu*; Sato, Takahiro; Tanaka, Akira*; Ishii, Keizo*
Nuclear Instruments and Methods in Physics Research B, 190(1-4), p.271 - 275, 2001/09
Times Cited Count：68 Percentile：95.85(Instruments & Instrumentation)no abstracts in English
Kamiya, Tomihiro; Sakai, Takuro; Oikawa, Masakazu*; Sato, Takahiro*; Ishii, Keizo*; Sugimoto, Asuka*; Matsuyama, Shigeo*
International Journal of PIXE, 9(3&4), p.217 - 225, 1999/11
no abstracts in English
Sakai, Takuro; ; Hirao, Toshio; Kamiya, Tomihiro; ; ; Matsuyama, Shigeo*; ; Ishii, Keizo*
Nuclear Instruments and Methods in Physics Research B, 136-138, p.390 - 394, 1998/00
Times Cited Count：18 Percentile：78.58(Instruments & Instrumentation)no abstracts in English
; Hirao, Toshio; Nashiyama, Isamu; Sakai, Takuro; Kamiya, Tomihiro; ; Ishii, Keizo*
Radiation Physics and Chemistry, 53(5), p.461 - 467, 1998/00
Times Cited Count：1 Percentile：14.80(Chemistry, Physical)no abstracts in English
Sakurai, Eiko*; Ishii, Keizo*; Yamanaka, Kentaro*; Okura, Satoru*; Fujiki, Kota*; Yamazaki, Hiromichi*; Matsuyama, Shigeo*; Kamiya, Tomihiro; Sato, Takahiro; Arakawa, Kazuo*; et al.
no journal, ,
Cognitive degradation and behavior disorder occur under the mental disease caused by stimulant drugs. In this study, it was continuously administered to mice. After that, neuropoiesis of dentate gyrus and trace elements in its brain were analyzed by in-air micro-PIXE. As a result, the distribution of trace elements was different from that of normal mice.
Sakurai, Eiko*; Yanai, Kazuhiko*; Okamura, Nobuyuki*; Ishii, Keizo*; Fujiki, Kota*; Hiraishi, Shingo*; Yamazaki, Hiromichi*; Matsuyama, Shigeo*; Yokoyama, Akihito; Kada, Wataru; et al.
no journal, ,
no abstracts in English
Sato, Takahiro; Koka, Masashi; Kada, Wataru; Yokoyama, Akihito; Okubo, Takeru; Yamazaki, Akiyoshi; Ishii, Yasuyuki; Kamiya, Tomihiro; Matsuyama, Shigeo*; Ishii, Keizo*
no journal, ,
In order to make elemental analysis in a minute cell, we developed in-air micro-PIXE analysis system in which light-ion microbeam of diameter 0.25m is used, and the imaging of the trace-element distribution was successfully carried out. This system is widely applied in the fields of medicine, biology, pharmacy, and dentistry.
Iwata, Yoshihiro*; Ishii, Keizo*; Yokota, Wataru; Kamiya, Tomihiro; Sato, Takahiro; Oikawa, Masakazu*
no journal, ,
no abstracts in English
Sakurai, Eiko*; Kuramasu, Atsuro*; Sato, Takahiro; Kamiya, Tomihiro; Ishii, Keizo*; Yanai, Kazuhiko*
no journal, ,
The elemental distribution in a brain section of a histamine-receptor-deficit mouse was measured by in-air micro-PIXE. The relation of monoamine in a brain and activity of the mouse suggested that the histamine receptor is a important part in a nervous system of monoamine.
Sakurai, Eiko*; Yanai, Kazuhiko*; Ishii, Keizo*; Sato, Takahiro; Kamiya, Tomihiro
no journal, ,
Methamphetamine was repeatedly administered to mice 1 mg/kg per day for seven days. After that, the brain sections containing a sea horse were analyzed by submilli-PIXE. As a result, iron accumulation was seen at a methamphetamine administration group.
Iwata, Yoshihiro*; Ishii, Keizo*; Sato, Takahiro; Kamiya, Tomihiro
no journal, ,
no abstracts in English
Endo, Kiyoshi*; Shibata, Yasushi*; Yamamoto, Tetsuya*; Matsumura, Akira*; Sato, Takahiro; Yokoyama, Akihito; Koka, Masashi; Okubo, Takeru; Yamazaki, Akiyoshi; Ishii, Yasuyuki; et al.
no journal, ,
no abstracts in English
