Real-time observation of irradiated HeLa-cell modified by fluorescent ubiquitination-based cell-cycle indicator using synchrotron X-ray microbeam

Narita, Ayumi; Kaminaga, Kiichi; Yokoya, Akinari; Noguchi, Miho; Kobayashi, Katsumi*; Usami, Noriko*; Fujii, Kentaro

Radiation Protection Dosimetry, 166(1-4), p.192 - 196, 2015/09

https://doi.org/10.1093/rpd/ncv156

For the knowledge about irradiation effects of mammalian cells depending on the cell cycle, most of them had been analyzed by statistical approches. Our purpose is to establish the method for real-time observation of irradiated cells under a microscope. Fluorescent ubiquitination-based cell cycle indicator (FUCCI) human cancer (HeLa) cells (red indicates G1; green, S/G2) were exposed to a synchrotron X-ray microbeam. Cells in either G1 or S/G2 were selectively irradiated according to cell color observed in the same microscopic field in a culture dish. Time-lapse micrographs of the irradiated cells were acquired for 24 h after irradiation. The cell cycle was strongly arrested by irradiation at S/G2 and never progressed to G1. In contrast, cells irradiated at G1 progress to S/G2 with a similar time course as non-irradiated control cells. These results show single FUCCI cell exposure and live cell imaging are powerful methods for studying radiation effects on the cell cycle.

Single-cell tracking and dynamical analysis of cellular population based on systems biology

Yokoya, Akinari; Kaminaga, Kiichi

Hoshasen Seibutsu Kenkyu, 49(4), p.418 - 431, 2014/12

no abstracts in English

Live-cell imaging study of mitochondrial morphology in mammalian cells irradiated

Kanari, Yukiko; Noguchi, Miho; Kaminaga, Kiichi; Sakamoto, Yuka; Yokoya, Akinari

Journal of Radiation Research, 55(Suppl.1), p.i129 - i130, 2014/03

https://doi.org/10.1093/jrr/rrt167

Live cell imaging of cell division of X-ray microbeam irradiated and non-irradiated cells

Kaminaga, Kiichi; Kanari, Yukiko; Sakamoto, Yuka; Narita, Ayumi; Usami, Noriko*; Kobayashi, Katsumi*; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English

Visualization of cell cycle arrest by X-irradiation in single Hela cells using fluorescent ubiquitination-based cell cycle indicator

Kaminaga, Kiichi; Noguchi, Miho; Narita, Ayumi; Sakamoto, Yuka; Kanari, Yukiko; Yokoya, Akinari

no journal, , 

Live cell imaging study on biological effects induced by X-ray microbeam irradiation

Kaminaga, Kiichi; Kanari, Yukiko*; Sakamoto, Yuka*; Noguchi, Miho; Narita, Ayumi*; Fujii, Kentaro; Usami, Noriko*; Kobayashi, Katsumi*; Suzuki, Keiji*; Yokoya, Akinari

no journal, , 

Ionizing radiation induced mitochondrial morphological change during the cell cycle

Noguchi, Miho; Kanari, Yukiko; Kaminaga, Kiichi; Sakamoto, Yuka; Narita, Ayumi; Fujii, Kentaro; Yokoya, Akinari

no journal, , 

Most studies of radiation induced biological effect starts from nuclear DNA damage. However, in the case of low dose irradiation, recent reports suggest that extranuclear targets, such as in cytoplasm, may have a role in mediating some important effects of radiation. Cellular effect of cytoplasmic irradiation remains to be elucidated. Mitochondria spread throughout the entire cytoplasm and contain their own genome, and mediate essential cell functions, such as generation of ATP and regulation of cell death. Mitochondria are main source of oxidative stress to generate ROS as by-product of respiration for ATP production. In addition, dysfunctions of mitochondria are involved in a wide variety of diseases. Mitochondrial functions are indispensable for survival. However, radiation effect of mitochondrial functions is not clear. In order to clarify what effects ionizing radiation leads to mitochondrial functions, we first examined the effects of ionizing radiation on mitochondrial morphology in mammalian cells. Mitochondria are dynamic organelles that continuously fuse and divide in response to environment and cellular differentiation. It is known that mitochondrial morphology dynamically change with cell cycle progression. In this study, after irradiation, we labeled mitochondria by Mitotracker Red and analyzed kinetics of mitochondrial morphology for 4 days by live-cell imaging techniques. Cell cycle stages were identified by nuclei staining. We reports the relation between radiation induced cell cycle arrest and mitochondrial dynamics.

Visualization of cell cycle modification by lionizing radiation in single HeLa calls using fluorescent ubiquitination-based cell cycle indicator

Kaminaga, Kiichi; Narita, Ayumi; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English

Live imaging of cells exposed to X-ray microbeam

Yokoya, Akinari; Narita, Ayumi; Kaminaga, Kiichi; Kanari, Yukiko; Sakamoto, Yuka; Noguchi, Miho; Usami, Noriko*; Kobayashi, Katsumi*; Fujii, Kentaro; Suzuki, Keiji*

no journal, , 

no abstracts in English

Low dose effect on spheroid of HeLa-Fucci cells exposed to cesium-containing Fukushima soil

Sakamoto, Yuka; Kanari, Yukiko; Kaminaga, Kiichi; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English