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Journal Articles

Visualisation of cell cycle modifications by X-ray irradiation of single HeLa cells using fluorescent ubiquitination-based cell cycle indicators

Kaminaga, Kiichi; Noguchi, Miho; Narita, Ayumi; Sakamoto, Yuka; Kanari, Yukiko; Yokoya, Akinari

Radiation Protection Dosimetry, 166(1-4), p.91 - 94, 2015/09

 Times Cited Count:5 Percentile:45.16(Environmental Sciences)

Journal Articles

Real-time observation of irradiated HeLa-cell modified by fluorescent ubiquitination-based cell-cycle indicator using synchrotron X-ray microbeam

Narita, Ayumi; Kaminaga, Kiichi; Yokoya, Akinari; Noguchi, Miho; Kobayashi, Katsumi*; Usami, Noriko*; Fujii, Kentaro

Radiation Protection Dosimetry, 166(1-4), p.192 - 196, 2015/09

 Times Cited Count:2 Percentile:73.66(Environmental Sciences)

For the knowledge about irradiation effects of mammalian cells depending on the cell cycle, most of them had been analyzed by statistical approches. Our purpose is to establish the method for real-time observation of irradiated cells under a microscope. Fluorescent ubiquitination-based cell cycle indicator (FUCCI) human cancer (HeLa) cells (red indicates G1; green, S/G2) were exposed to a synchrotron X-ray microbeam. Cells in either G1 or S/G2 were selectively irradiated according to cell color observed in the same microscopic field in a culture dish. Time-lapse micrographs of the irradiated cells were acquired for 24 h after irradiation. The cell cycle was strongly arrested by irradiation at S/G2 and never progressed to G1. In contrast, cells irradiated at G1 progress to S/G2 with a similar time course as non-irradiated control cells. These results show single FUCCI cell exposure and live cell imaging are powerful methods for studying radiation effects on the cell cycle.

Journal Articles

Single-cell tracking and dynamical analysis of cellular population based on systems biology

Yokoya, Akinari; Kaminaga, Kiichi

Hoshasen Seibutsu Kenkyu, 49(4), p.418 - 431, 2014/12

no abstracts in English

Journal Articles

Live-cell imaging study of mitochondrial morphology in mammalian cells irradiated

Kanari, Yukiko; Noguchi, Miho; Kaminaga, Kiichi; Sakamoto, Yuka; Yokoya, Akinari

Journal of Radiation Research, 55(Suppl.1), p.i129 - i130, 2014/03

Journal Articles

Visualization of cell-cycle modification by ionizing irradiation in single HeLa cells using fluorescent ubiquitination-based cell-cycle indicator

Kaminaga, Kiichi; Sakamoto, Yuka; Kanari, Yukiko; Noguchi, Miho; Yokoya, Akinari

Journal of Radiation Research, 55(Suppl.1), p.i127 - i128, 2014/03

Journal Articles

Three-dimensional culture of HeLa-FUCCI cells for study of bystander cell cycle effect of high LET particles

Sakamoto, Yuka; Kaminaga, Kiichi; Kanari, Yukiko; Noguchi, Miho; Yokoya, Akinari

Journal of Radiation Research, 55(Suppl.1), p.i120 - i121, 2014/03

Oral presentation

Time-lapse imaging technique applied to study of X-irradiation effect on cell-cycle of Fucci-expressing cells

Yokoya, Akinari; Noguchi, Miho; Kaminaga, Kiichi; Sakamoto, Yuka; Kanari, Yukiko; Narita, Ayumi; Fujii, Kentaro

no journal, , 

no abstracts in English

Oral presentation

Ionizing radiation induced mitochondrial morphological change during the cell cycle

Noguchi, Miho; Kanari, Yukiko; Kaminaga, Kiichi; Sakamoto, Yuka; Narita, Ayumi; Fujii, Kentaro; Yokoya, Akinari

no journal, , 

Most studies of radiation induced biological effect starts from nuclear DNA damage. However, in the case of low dose irradiation, recent reports suggest that extranuclear targets, such as in cytoplasm, may have a role in mediating some important effects of radiation. Cellular effect of cytoplasmic irradiation remains to be elucidated. Mitochondria spread throughout the entire cytoplasm and contain their own genome, and mediate essential cell functions, such as generation of ATP and regulation of cell death. Mitochondria are main source of oxidative stress to generate ROS as by-product of respiration for ATP production. In addition, dysfunctions of mitochondria are involved in a wide variety of diseases. Mitochondrial functions are indispensable for survival. However, radiation effect of mitochondrial functions is not clear. In order to clarify what effects ionizing radiation leads to mitochondrial functions, we first examined the effects of ionizing radiation on mitochondrial morphology in mammalian cells. Mitochondria are dynamic organelles that continuously fuse and divide in response to environment and cellular differentiation. It is known that mitochondrial morphology dynamically change with cell cycle progression. In this study, after irradiation, we labeled mitochondria by Mitotracker Red and analyzed kinetics of mitochondrial morphology for 4 days by live-cell imaging techniques. Cell cycle stages were identified by nuclei staining. We reports the relation between radiation induced cell cycle arrest and mitochondrial dynamics.

Oral presentation

Live-cell imaging study of mitochondrial morphology in mammalian cells exposed to X-rays

Noguchi, Miho; Kanari, Yukiko; Kaminaga, Kiichi; Sakamoto, Yuka; Narita, Ayumi; Fujii, Kentaro; Yokoya, Akinari

no journal, , 

Oral presentation

New approach for study cell cycle modification of HeLa cells by irradiation usimg synchrotron X-ray microbeam

Narita, Ayumi; Kaminaga, Kiichi; Noguchi, Miho; Yokoya, Akinari; Kobayashi, Katsumi*; Usami, Noriko*; Fujii, Kentaro

no journal, , 

no abstracts in English

Oral presentation

Effect of X-irradiation to Fucci cells on cell cycle observed by time-lapse imaging technique

Kaminaga, Kiichi; Narita, Ayumi; Noguchi, Miho; Kobayashi, Katsumi*; Usami, Noriko*; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Low dose effect on spheroid of HeLa-Fucci cells exposed to cesium-containing Fukushima soil

Sakamoto, Yuka; Kanari, Yukiko; Kaminaga, Kiichi; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Effect of X-ray irradiation for Fucci-cells observed by timelapse imaging

Kaminaga, Kiichi; Narita, Ayumi; Noguchi, Miho; Kanari, Yukiko; Sakamoto, Yuka; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Cell cycle modification of Fucci-HeLa cells induced by X-irradiation

Kaminaga, Kiichi; Narita, Ayumi; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Visualization of cell cycle modification by lionizing radiation in single HeLa calls using fluorescent ubiquitination-based cell cycle indicator

Kaminaga, Kiichi; Narita, Ayumi; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Observation of cell cycle effect using X-ray microbeam

Narita, Ayumi; Kaminaga, Kiichi; Noguchi, Miho; Yokoya, Akinari; Kobayashi, Katsumi*; Usami, Noriko*; Fujii, Kentaro

no journal, , 

no abstracts in English

Oral presentation

Relation between mitochondrial dynamic state change and membrane potential by X-ray irradiation

Kanari, Yukiko; Noguchi, Miho; Kaminaga, Kiichi; Sakamoto, Yuka; Yokoya, Akinari; Suzuki, Keiji*

no journal, , 

no abstracts in English

Oral presentation

Effect of X-ray microbeam for Fucci cells observed by time-lapse imaging

Kaminaga, Kiichi; Narita, Ayumi; Noguchi, Miho; Kanari, Yukiko; Sakamoto, Yuka; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Three-dimensional Culture of HeLa-Fucci Cells for Study of Bystander Effect through gap-junction

Sakamoto, Yuka; Kanari, Yukiko; Kaminaga, Kiichi; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Real-time observation for irradiation effect depending on cell-cycle using X-ray microbeam

Narita, Ayumi; Kaminaga, Kiichi; Yokoya, Akinari; Noguchi, Miho; Kobayashi, Katsumi*; Usami, Noriko*; Fujii, Kentaro

no journal, , 

no abstracts in English

34 (Records 1-20 displayed on this page)