Real-time observation of irradiated HeLa-cell modified by fluorescent ubiquitination-based cell-cycle indicator using synchrotron X-ray microbeam

Narita, Ayumi; Kaminaga, Kiichi; Yokoya, Akinari; Noguchi, Miho; Kobayashi, Katsumi*; Usami, Noriko*; Fujii, Kentaro

Radiation Protection Dosimetry, 166(1-4), p.192 - 196, 2015/09

https://doi.org/10.1093/rpd/ncv156

For the knowledge about irradiation effects of mammalian cells depending on the cell cycle, most of them had been analyzed by statistical approches. Our purpose is to establish the method for real-time observation of irradiated cells under a microscope. Fluorescent ubiquitination-based cell cycle indicator (FUCCI) human cancer (HeLa) cells (red indicates G1; green, S/G2) were exposed to a synchrotron X-ray microbeam. Cells in either G1 or S/G2 were selectively irradiated according to cell color observed in the same microscopic field in a culture dish. Time-lapse micrographs of the irradiated cells were acquired for 24 h after irradiation. The cell cycle was strongly arrested by irradiation at S/G2 and never progressed to G1. In contrast, cells irradiated at G1 progress to S/G2 with a similar time course as non-irradiated control cells. These results show single FUCCI cell exposure and live cell imaging are powerful methods for studying radiation effects on the cell cycle.

Three-dimensional culture of HeLa-FUCCI cells for study of bystander cell cycle effect of high LET particles

Sakamoto, Yuka; Kaminaga, Kiichi; Kanari, Yukiko; Noguchi, Miho; Yokoya, Akinari

Journal of Radiation Research, 55(Suppl.1), p.i120 - i121, 2014/03

https://doi.org/10.1093/jrr/rrt166

Visualization of cell-cycle modification by ionizing irradiation in single HeLa cells using fluorescent ubiquitination-based cell-cycle indicator

Kaminaga, Kiichi; Sakamoto, Yuka; Kanari, Yukiko; Noguchi, Miho; Yokoya, Akinari

Journal of Radiation Research, 55(Suppl.1), p.i127 - i128, 2014/03

https://doi.org/10.1093/jrr/rrt159

Effect on mitochondrial activity by partial exposure to living cells using X-ray microbeam

Kanari, Yukiko; Kaminaga, Kiichi; Sakamoto, Yuka; Narita, Ayumi; Noguchi, Miho; Usami, Noriko*; Kobayashi, Katsumi*; Suzuki, Keiji*; Yokoya, Akinari; Fujii, Kentaro

no journal, , 

no abstracts in English

Elucidation of bystander effect to cell cycle

Kaminaga, Kiichi; Narita, Ayumi; Noguchi, Miho; Kanari, Yukiko; Sakamoto, Yuka; Usami, Noriko*; Yokoya, Akinari

no journal, , 

no abstracts in English

Live cell imaging of cells exposed to ionizing radiation and computer simulation of the cell cycle modulations

Yokoya, Akinari; Kaminaga, Kiichi; Hattori, Yuya; Watanabe, Ritsuko; Noguchi, Miho; Fujii, Kentaro

no journal, , 

no abstracts in English

Time-lapse imaging of cell cycle progression after X-ray microbeam exposure to mammalian cells

Kaminaga, Kiichi; Usami, Noriko*; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English

Effect of X-ray irradiation for Fucci-cells observed by timelapse imaging

Kaminaga, Kiichi; Narita, Ayumi; Noguchi, Miho; Kanari, Yukiko; Sakamoto, Yuka; Yokoya, Akinari

no journal, , 

no abstracts in English

Cell cycle modification of Fucci-HeLa cells induced by X-irradiation

Kaminaga, Kiichi; Narita, Ayumi; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English

Real-time observation for irradiation effect depending on cell-cycle using X-ray microbeam

Narita, Ayumi; Kaminaga, Kiichi; Yokoya, Akinari; Noguchi, Miho; Kobayashi, Katsumi*; Usami, Noriko*; Fujii, Kentaro

no journal, , 

no abstracts in English