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JAEA Reports

Case studies of radiation dose assessment in emergency situation of nuclear facilities

Kawasaki, Masatsugu; Nakajima, Junya; Yoshida, Keisuke; Kato, Saori; Nishino, Sho; Nozaki, Teo; Nakagawa, Masahiro; Tsunoda, Junichi; Sugaya, Yuki; Hasegawa, Rie; et al.

JAEA-Data/Code 2017-004, 57 Pages, 2017/03

JAEA-Data-Code-2017-004.pdf:2.34MB

In emergency situation of nuclear facilities, we need to estimate the radiation dose due to radiation and radioactivity to grasp the influence range of the accident in the early stage. Therefore, we prepare the case studies of dose assessment for public exposure dose and personal exposure dose and contribute them to emergency procedures. This document covers about accidents of nuclear facilities in Nuclear Science Research Institute and past accident of nuclear power plant, and it can be used for inheritance of techniques of emergency dose assessment.

Journal Articles

Live-cell imaging study of mitochondrial morphology in mammalian cells exposed to X-rays

Noguchi, Miho; Kanari, Yukiko; Yokoya, Akinari; Narita, Ayumi; Fujii, Kentaro

Radiation Protection Dosimetry, 166(1-4), p.101 - 103, 2015/09

 Times Cited Count:0 Percentile:100(Environmental Sciences)

Journal Articles

Visualisation of cell cycle modifications by X-ray irradiation of single HeLa cells using fluorescent ubiquitination-based cell cycle indicators

Kaminaga, Kiichi; Noguchi, Miho; Narita, Ayumi; Sakamoto, Yuka; Kanari, Yukiko; Yokoya, Akinari

Radiation Protection Dosimetry, 166(1-4), p.91 - 94, 2015/09

 Times Cited Count:5 Percentile:44.04(Environmental Sciences)

Journal Articles

Live-cell imaging study of mitochondrial morphology in mammalian cells irradiated

Kanari, Yukiko; Noguchi, Miho; Kaminaga, Kiichi; Sakamoto, Yuka; Yokoya, Akinari

Journal of Radiation Research, 55(Suppl.1), p.i129 - i130, 2014/03

Journal Articles

Visualization of cell-cycle modification by ionizing irradiation in single HeLa cells using fluorescent ubiquitination-based cell-cycle indicator

Kaminaga, Kiichi; Sakamoto, Yuka; Kanari, Yukiko; Noguchi, Miho; Yokoya, Akinari

Journal of Radiation Research, 55(Suppl.1), p.i127 - i128, 2014/03

Journal Articles

Three-dimensional culture of HeLa-FUCCI cells for study of bystander cell cycle effect of high LET particles

Sakamoto, Yuka; Kaminaga, Kiichi; Kanari, Yukiko; Noguchi, Miho; Yokoya, Akinari

Journal of Radiation Research, 55(Suppl.1), p.i120 - i121, 2014/03

Oral presentation

Time-lapse imaging technique applied to study of X-irradiation effect on cell-cycle of Fucci-expressing cells

Yokoya, Akinari; Noguchi, Miho; Kaminaga, Kiichi; Sakamoto, Yuka; Kanari, Yukiko; Narita, Ayumi; Fujii, Kentaro

no journal, , 

no abstracts in English

Oral presentation

Ionizing radiation induced mitochondrial morphological change during the cell cycle

Noguchi, Miho; Kanari, Yukiko; Kaminaga, Kiichi; Sakamoto, Yuka; Narita, Ayumi; Fujii, Kentaro; Yokoya, Akinari

no journal, , 

Most studies of radiation induced biological effect starts from nuclear DNA damage. However, in the case of low dose irradiation, recent reports suggest that extranuclear targets, such as in cytoplasm, may have a role in mediating some important effects of radiation. Cellular effect of cytoplasmic irradiation remains to be elucidated. Mitochondria spread throughout the entire cytoplasm and contain their own genome, and mediate essential cell functions, such as generation of ATP and regulation of cell death. Mitochondria are main source of oxidative stress to generate ROS as by-product of respiration for ATP production. In addition, dysfunctions of mitochondria are involved in a wide variety of diseases. Mitochondrial functions are indispensable for survival. However, radiation effect of mitochondrial functions is not clear. In order to clarify what effects ionizing radiation leads to mitochondrial functions, we first examined the effects of ionizing radiation on mitochondrial morphology in mammalian cells. Mitochondria are dynamic organelles that continuously fuse and divide in response to environment and cellular differentiation. It is known that mitochondrial morphology dynamically change with cell cycle progression. In this study, after irradiation, we labeled mitochondria by Mitotracker Red and analyzed kinetics of mitochondrial morphology for 4 days by live-cell imaging techniques. Cell cycle stages were identified by nuclei staining. We reports the relation between radiation induced cell cycle arrest and mitochondrial dynamics.

Oral presentation

Live-cell imaging study of mitochondrial morphology in mammalian cells exposed to X-rays

Noguchi, Miho; Kanari, Yukiko; Kaminaga, Kiichi; Sakamoto, Yuka; Narita, Ayumi; Fujii, Kentaro; Yokoya, Akinari

no journal, , 

Oral presentation

Low dose effect on spheroid of HeLa-Fucci cells exposed to cesium-containing Fukushima soil

Sakamoto, Yuka; Kanari, Yukiko; Kaminaga, Kiichi; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Effect of X-ray irradiation for Fucci-cells observed by timelapse imaging

Kaminaga, Kiichi; Narita, Ayumi; Noguchi, Miho; Kanari, Yukiko; Sakamoto, Yuka; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Relation between morphological changes of mitochondria and radiosensitivity

Noguchi, Miho; Kanari, Yukiko; Fujii, Kentaro; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Dynamic effect on mitochondria membrane potential induced by X-ray irradiation

Kanari, Yukiko; Noguchi, Miho; Fujii, Kentaro; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Live-cell imaging study of mitochondrial morphology in mammalian cells irradiated

Kanari, Yukiko; Noguchi, Miho; Fujii, Kentaro; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Relation between mitochondrial dynamic state change and membrane potential by X-ray irradiation

Kanari, Yukiko; Noguchi, Miho; Kaminaga, Kiichi; Sakamoto, Yuka; Yokoya, Akinari; Suzuki, Keiji*

no journal, , 

no abstracts in English

Oral presentation

Effect of X-ray microbeam for Fucci cells observed by time-lapse imaging

Kaminaga, Kiichi; Narita, Ayumi; Noguchi, Miho; Kanari, Yukiko; Sakamoto, Yuka; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Three-dimensional Culture of HeLa-Fucci Cells for Study of Bystander Effect through gap-junction

Sakamoto, Yuka; Kanari, Yukiko; Kaminaga, Kiichi; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Live cell imaging of mitosis of cells exposed to X-ray microbeam

Kaminaga, Kiichi; Kanari, Yukiko; Sakamoto, Yuka; Narita, Ayumi; Usami, Noriko*; Kobayashi, Katsumi*; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English

Oral presentation

Live imaging of cells exposed to X-ray microbeam

Yokoya, Akinari; Narita, Ayumi; Kaminaga, Kiichi; Kanari, Yukiko; Sakamoto, Yuka; Noguchi, Miho; Usami, Noriko*; Kobayashi, Katsumi*; Fujii, Kentaro; Suzuki, Keiji*

no journal, , 

no abstracts in English

Oral presentation

Live cell imaging of cell division of X-ray microbeam irradiated and non-irradiated cells

Kaminaga, Kiichi; Kanari, Yukiko; Sakamoto, Yuka; Narita, Ayumi; Usami, Noriko*; Kobayashi, Katsumi*; Noguchi, Miho; Yokoya, Akinari

no journal, , 

no abstracts in English

27 (Records 1-20 displayed on this page)