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Journal Articles

Structure of a highly acidic $$beta$$-lactamase from the moderate halophile ${it Chromohalobacter}$ sp.560 and the discovery of a Cs$$^{+}$$-selective binding site

Arai, Shigeki; Yonezawa, Yasushi*; Okazaki, Nobuo*; Matsumoto, Fumiko*; Shibazaki, Chie; Shimizu, Rumi; Yamada, Mitsugu*; Adachi, Motoyasu; Tamada, Taro; Kawamoto, Masahide*; et al.

Acta Crystallographica Section D, 71(3), p.541 - 554, 2015/03

https://doi.org/10.1107/S1399004714027734
 Times Cited Count：7 Percentile：50.44(Biochemical Research Methods)

The crystal structure of halophilic $$beta$$-lactamase from ${it Chromohalobacter}$ sp.560 (HaBLA) was determined using X-ray crystallography. Moreover, the locations of bound Sr$$^{2+}$$ and Cs$$^{+}$$ ions were identified by anomalous X-ray diffraction. The location of one Cs$$^{+}$$ specific binding site was identified on HaBLA even in the presence of 9-fold molar excess of Na$$^{+}$$ (90 mM Na$$^{+}$$ /10 mM Cs$$^{+}$$). This Cs$$^{+}$$ binding site is formed by two main-chain O atoms and an aromatic ring of a side chain of Trp. An aromatic ring of Trp interacts with Cs$$^{+}$$ by the cation-$$pi$$ interaction. The observation of a selective and high-affinity Cs$$^{+}$$ binding site provides important information that is useful for designing artificial Cs$$^{+}$$ binding sites useful in bioremediation of radioactive isotopes.

Oral presentation

Discovery of Cs selective binding site on a halophilic beta-lactamase by the anomalous X-ray diffraction analysis

Arai, Shigeki; Adachi, Motoyasu; Kawamoto, Masahide*; Tokunaga, Hiroko*; Ishibashi, Matsujiro*; Tokunaga, Masao*; Kuroki, Ryota

no journal, , 

We attempted to discover the Cs$$^{+}$$ and Sr$$^{2+}$$ binding sites on the halophilic $$beta$$-Lactamase (HaBLA) derived from ${it Chromohalobacter}$ sp.560 by the anomalous X-ray diffraction analysis. One Cs$$^{+}$$ ions in the HaBLA crystal were identified by BL7 at SAGA-LS. Three Sr$$^{2+}$$ ions in the HaBLA crystal were identified by BL38B1 at SPring-8 and NW12A at Photon Factory. Discovered Cs$$^{+}$$ binding site can bind Cs$$^{+}$$ selectively from a solution containing Na$$^{+}$$/Cs$$^{+}$$ = 90 mM/10 mM. This Cs$$^{+}$$ binding site is formed by two main-chain O atoms and an aromatic ring of a side chain of Trp. An aromatic ring of Trp interacts with Cs$$^{+}$$ by the cation-$$pi$$ interaction. The observation of metal binding sites with relatively higher Cs$$^{+}$$ selectivity provides important information that is useful for designing artificial Cs$$^{+}$$ binding sites.

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