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Journal Articles

Effect of ionizing radiation upon dehydrated Pv11 cultured cells originated from the sleeping chironomid

Watanabe, Kazuyo*; Akitsuki, Takashi*; Shimura, Sachiko*; Gusev, O.*; Cornette, R.*; Kikawada, Takahiro*; Sakashita, Tetsuya; Funayama, Tomoo; Kobayashi, Yasuhiko; Okuda, Takashi*

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 87, 2015/03

The Sleeping Chironomid, ${it Polypedilum vanderplanki}$ can stand complete desiccation (anhydrobiosis) and also shows radio-resistance. Recently, we have generated cultured cell (Pv11) originated from ${it P. vanderplanki}$ embryo which can also stand complete dehydration. In this study, we examine the tolerance of cultured cell Pv11 against ionbeam irradiation.

Journal Articles

Anhydrobiosis-associated nuclear DNA damage and repair in the Sleeping Chironomid; Linkage with radioresistance

Gusev, O.*; Nakahara, Yuichi*; Vanyagina, V.*; Malutina, L.*; Cornette, R.*; Sakashita, Tetsuya; Hamada, Nobuyuki*; Kikawada, Takahiro*; Kobayashi, Yasuhiko; Okuda, Takashi*

PLoS ONE (Internet), 5(11), p.e14008_1 - e14008_9, 2010/11

 Times Cited Count:42 Percentile:73.43(Multidisciplinary Sciences)

Anhydrobiotic chironomid larvae can withstand prolonged complete desiccation as well as other external stresses including ionizing radiation. To understand the cross-tolerance mechanism, we have analyzed the damage and repair in the nuclear DNA using DNA comet assays and gene expression in relation to anhydrobiosis and radiation. We found that dehydration causes alterations in chromatin structure and a severe fragmentation of nuclear DNA in the cells of the larvae despite successful anhydrobiosis. Furthermore, while the larvae have restored physiological activity within an hour following rehydration, nuclear DNA restoration typically took 72 to 96 h.

Journal Articles

Radiation tolerance linked to anhydrobiosis in ${it Polypedilum vanderplanki}$

Nakahara, Yuichi*; Watanabe, Masahiko*; Kikawada, Takahiro*; Fujita, Akihiko*; Horikawa, Daiki*; Okuda, Takashi*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Wada, Seiichi*; et al.

JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 113, 2008/03

We have shown that anhydrobiotic larvae of ${it Polypedilum vanderplanki}$ have higher tolerance against both high linear energy transfer (LET) radiation than hydrated larvae. We therefore examined effects of high-LET radiation on four kinds of larvae: (1) normal hydrated (intact) larva, (2) intermediates between the anhydrobiotic and normal hydrated state, (3) almost completely dehydrated (anhydrobiotic) larvae, and (4) immediately rehydrated larvae that are assumed to have a similar molecular profile to anhydrobiotic larvae. The intermediates and immediately rehydrated larvae survived longer after high-LET radiation than intact larvae, indicating that radiation tolerance could be enhanced even in hydrated larvae. Physiological changes toward anhydrobiosis, e.g. accumulation of protectants or increasing damage repair capacity, correlate with improved radiation tolerance in hydrated larvae.

Journal Articles

Physiological changes leading to anhydrobiosis improve radiation tolerance in ${it Polypedilum vanderplanki}$ larvae

Watanabe, Masahiko*; Nakahara, Yuichi*; Sakashita, Tetsuya; Kikawada, Takahiro*; Fujita, Akihiko*; Hamada, Nobuyuki*; Horikawa, Daiki*; Wada, Seiichi*; Kobayashi, Yasuhiko; Okuda, Takashi*

Journal of Insect Physiology, 53(6), p.573 - 579, 2007/06

 Times Cited Count:19 Percentile:67.58(Entomology)

We examined effects of high-LET radiation on 4 kinds of larvae: (1) normal hydrated (intact) larva, (2) intermediates between the anhydrobiotic and normal hydrated state, (3) almost completely dehydrated (anhydrobiotic) larvae, and (4) immediately-rehydrated larvae that are assumed to have a similar molecular profile to anhydrobiotic larvae. The intermediates and immediately-rehydrated larvae survived longer after high-LET radiation than intact larvae, indicating that radiation tolerance could be enhanced even in hydrated larvae. Physiological changes toward anhydrobiosis, e.g. accumulation of protectants or increasing damage repair capacity, correlate with improved radiation tolerance in hydrated larvae. In addition, almost complete desiccation further enhanced radiation tolerance, possibly in a different way from the hydrated larvae.

Journal Articles

Effects of heavy ions and $$gamma$$-ray on the tardigrade ${it Milnesium tardigradum}$

Horikawa, Daiki*; Sakashita, Tetsuya; Katagiri, Chihiro*; Watanabe, Masahiko*; Kikawada, Takahiro*; Nakahara, Yuichi*; Hamada, Nobuyuki*; Wada, Seiichi*; Funayama, Tomoo; Higashi, Seigo*; et al.

JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 116, 2007/02

no abstracts in English

Journal Articles

Radiation tolerance in the tardigrade ${it Milnesium tardigradum}$

Horikawa, Daiki*; Sakashita, Tetsuya; Katagiri, Chihiro*; Watanabe, Masahiko*; Kikawada, Takahiro*; Nakahara, Yuichi*; Hamada, Nobuyuki*; Wada, Seiichi*; Funayama, Tomoo; Higashi, Seigo*; et al.

International Journal of Radiation Biology, 82(12), p.843 - 848, 2006/12

 Times Cited Count:110 Percentile:99.11(Biology)

no abstracts in English

Journal Articles

Estimation of radiation tolerance to high LET heavy ions in an anhydrobiotic insect, ${it Polypedilum vanderplanki}$

Watanabe, Masahiko*; Sakashita, Tetsuya; Fujita, Akihiko*; Kikawada, Takahiro*; Nakahara, Yuichi*; Hamada, Nobuyuki*; Horikawa, Daiki*; Wada, Seiichi*; Funayama, Tomoo; Kobayashi, Yasuhiko; et al.

International Journal of Radiation Biology, 82(12), p.835 - 842, 2006/12

 Times Cited Count:15 Percentile:73.49(Biology)

The aim of this study is to characterize the tolerance to high-LET radiations of${it P. vanderplanki}$. Larval survival and subsequent metamorphoses were compared between anhydrobiotic (dry) and non-anhydrobiotic (wet) samples after exposure to 1 to 7000 Gy of three types of heavy ions with LET values ranging from 16.2 to 321 keV/$$mu$$m. At all LET values measured, dry larvae consistently showed greater radiation tolerance than hydrated larvae, due to the disaccharide trehalose in anhydrobiotic animals. Relative biological effectiveness (RBE) values based on the median inhibitory doses reached a maximum at 116 keV/$$mu$$m ($$^{12}$$C). Anhydrobiosis potentiates radiation tolerance in terms of larval survival, pupation and adult emergence of ${it P. vanderplanki}$ exposed to high-LET radiations. ${it P. vanderplanki}$ larvae might have more efficient DNA damage repair after radiation than other chironomid species.

Journal Articles

Biological effects of anhydrobiosis in an African chironomid, ${it Polypedilum vanderplanki}$ on radiation tolerance

Watanabe, Masahiko*; Sakashita, Tetsuya; Fujita, Akihiko*; Kikawada, Takahiro*; Horikawa, Daiki*; Nakahara, Yuichi*; Wada, Seiichi*; Funayama, Tomoo; Hamada, Nobuyuki*; Kobayashi, Yasuhiko; et al.

International Journal of Radiation Biology, 82(8), p.587 - 592, 2006/08

 Times Cited Count:30 Percentile:88.8(Biology)

The present study aims to evaluate effects of anhydrobiosis on radiation tolerance in an anhydrobiotic insect, Polypedilum vanderplanki. Larval survival (48 h), anhydrobiotic ability, metamorphosis and reproduction after exposure to 1 to 9000 Gy of $$gamma$$-rays at the larval stage were comparedbetween anhydrobiotic (dry) and normal (wet) phases. Wet larvae were killed in a dose-dependent manner at doses higher than 2000 Gy, and all died within 8 h after 4000 Gy exposure. In contrast, dry larvae survived even 5000 Gy, and some of them still tolerated 7000 Gy and were alive at 48 h after rehydration. Moreover, greater radiotolerance of dry larva, compared to wet ones, was demonstrated interms of metamorphoses. However, anhydrobiosis did not protect against radiation damage in terms of producing viable offspring. These results indicate that anhydrobiosis enhances radiotolerance, resulting in increases of successful metamorphoses.

Oral presentation

Energetic heavy-ion beams and $$gamma$$-rays tolerance in water bears

Horikawa, Daiki*; Watanabe, Masahiko*; Nakahara, Yuichi*; Kikawada, Takahiro*; Okuda, Takashi*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Wada, Seiichi*; Kobayashi, Yasuhiko; et al.

no journal, , 

no abstracts in English

Oral presentation

Radiation tolerance in tardigrades

Horikawa, Daiki*; Sakashita, Tetsuya; Katagiri, Chihiro*; Watanabe, Masahiko*; Nakahara, Yuichi*; Kikawada, Takahiro*; Hamada, Nobuyuki*; Wada, Seiichi*; Funayama, Tomoo; Higashi, Seigo*; et al.

no journal, , 

no abstracts in English

Oral presentation

Radiation tolerance and anhydrobiosis in African chironomid, ${it Polypedilum vanderplanki}$

Nakahara, Yuichi*; Watanabe, Masahiko*; Sakashita, Tetsuya; Hamada, Nobuyuki*; Gusev, O.*; Fujita, Akihiko*; Kikawada, Takahiro*; Horikawa, Daiki*; Kobayashi, Yasuhiko; Okuda, Takashi*

no journal, , 

The present study aims to evaluate effects of anhydrobiosis on radiation tolerance in an anhydrobiotic insect, ${it Polypedilum vanderplanki}$. Wet larvae were killed in a dose-dependent manner at doses higher than 2000 Gy, and all died within 8 h after 4000 Gy exposure. However, anhydrobiosis did not protect against radiation damage in terms of producing viable offspring. These results indicate that anhydrobiosis enhances radiotolerance in larvae, but not in the delayed effects of development.

11 (Records 1-11 displayed on this page)
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