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Uchijima, Tomoki*; Kato, Shingo*; Tanimoto, Kazuya*; Shiraishi, Fumito*; Hamamura, Natsuko*; Tokunaga, Kohei; Makita, Hiroko*; Kondo, Momoko*; Okuma, Moriya*; Mitsunobu, Satoshi*
FEMS Microbiology Ecology, 101(6), p.fiaf051_1 - fiaf051_15, 2025/05
Chemolithotrophic neutrophilic iron (Fe)-oxidizing bacteria, which mainly belong to the family Gallionellaceae, universally prevail in terrestrial environments changing Fe cycling. However, they are typically recognized as difficult-to-culture microbes. Despite efforts, there are few Fe(II)-oxidizing lithotroph isolates; hence, their physiological and ecological knowledge remains limited. This limitation is largely owing to difficulties in their cultivation, and we hypothesize that the difficulty exists because substrate and mineral concentrations in the cultivation medium are not tuned to a specific environmental condition under which these organisms live. To address this hypothesis, this study proposes a novel custom-made medium approach for chemolithotrophic Fe(II)-oxidizing bacteria; a method that manipulates medium components through diligent analysis of field environment. A new custom-made medium simulating energy substrates and nutrients under the field condition was prepared by modifying both chemical composition and physical setup in the glass-tube medium. In particular, the modification of the physical setup in the tube had a significant effect on adjusting dissolved Fe(II) and O
concentrations to the field environment. Using the medium, Gallionellaceae members were successfully enriched and a new Gallionellaceae species was isolated from a natural hot spring site. Compared with conventional medium, the custom-made medium has significantly higher ability in enriching Gallionellaceae members.
Oka, Yoshihide*; Tsumori, Katsuyoshi*; Ikeda, Katsunori*; Kaneko, Osamu*; Nagaoka, Kenichi*; Osakabe, Masaki*; Takeiri, Yasuhiko*; Asano, Eiji*; Komada, Seiji*; Kondo, Tomoki*; et al.
Review of Scientific Instruments, 79(2), p.02C105_1 - 02C105_4, 2008/02
Times Cited Count：0 Percentile：0.00(Instruments & Instrumentation)In the present studies, we studied the cesium lines in the source plasma during beam shots on the LND MN-NBI system. It was found for the first time in the LHD-source 2, that both the amount of Cs I (neutral Cs) and Cs II (Cs
) in the source plasma light rose sharply when beam acceleration began, and continued rising during a 10 s pulse. We think that this was because the cesium was evaporated/sputtered from the source backplate by the back-streaming positive ions.
Kaku, Eiji*; Okamoto, Koji*; Kondo, Masahiro*; Ozdemir, E.*; Shiba, Tomoki*; Sato, Ikken
no journal, ,
In this study aiming at contribution for safe decommissioning of Fukushima-Daiichi NPP, CFD (Computational Fluid Dynamics) method was applied and temperature distribution of Unit 3 was reproduced. This temperature distribution was then compared with the measured data obtained by TEPCO so that debris distribution can be estimated. Combined application of optimized tools and CFD method to resolve inverse problem determining best suited thermal balance within the containment vessel is a characteristic of this study.
