Identification of erythroid progenitors induced by erythropoietic activity in

Kosaka, Nami*; Sugai, Tatsuhisa*; Nagasawa, Kazumichi*; Tanizaki, Yuta*; Meguro, Mizue; Aizawa, Yoichi*; Maekawa, Shun*; Adachi, Motoyasu; Kuroki, Ryota; Kato, Takashi

Journal of Experimental Biology, 214(6), p.921 - 927, 2011/03

https://doi.org/10.1242/jeb.050286

Oxygen is essential for the survival of animals. Red blood cells are responsible for transporting oxygen to tissues. We established a semi-solid colony forming assay, and showed that recombinant xlEPO induces erythroid colony formation in vitro and detected an increased level of erythropoietin activity in blood serum during acute anemic stress. In addition, the study demonstrated the possible presence of multiple, non-xlEPO, factors in anemic serum supportive of erythroid colony formation. These results indicate that erythropoiesis mediated by erythropoietin is present in amphibian species and, furthermore, that the regulatory mechanisms controlling peripheral erythrocyte number may vary among vertebrates.

Implication of molecular diversity and functional conservation of erythropoietin based on a comparison of tertiary structures of humans and frogs

Meguro, Mizue; Adachi, Motoyasu; Nagasawa, Kazumichi*; Beppu, Miho*; Okazaki, Nobuo; Kosaka, Nami*; Tamada, Taro; Kuroki, Ryota; Kato, Takashi

no journal, ,