Refine your search:     
Report No.
 - 
Search Results: Records 1-1 displayed on this page of 1
  • 1

Presentation/Publication Type

Initialising ...

Refine

Journal/Book Title

Initialising ...

Meeting title

Initialising ...

First Author

Initialising ...

Keyword

Initialising ...

Language

Initialising ...

Publication Year

Initialising ...

Held year of conference

Initialising ...

Save select records

Journal Articles

Structural and functional characterization of the ${it Geobacillus}$ copper nitrite reductase; Involvement of the unique N-terminal region in the interprotein electron transfer with its redox partner

Fukuda, Yota*; Koteishi, Hiroyasu*; Yoneda, Ryohei*; Tamada, Taro; Takami, Hideto*; Inoue, Tsuyoshi*; Nojiri, Masaki

Biochimica et Biophysica Acta; Bioenergetics, 1837(3), p.396 - 405, 2014/03

 Times Cited Count:15 Percentile:47.42(Biochemistry & Molecular Biology)

The crystal structures of copper-containing nitrite reductase (CuNiR) from the thermophilic Gram-positive bacterium ${it Geobacillus kaustophilus}$ HTA426 and the amino (N)-terminal 68 residue-deleted mutant were determined at resolutions of 1.3${AA}$ and 1.8${AA}$, respectively. Both structures show a striking resemblance with the overall structure of the well-known CuNiRs composed of two Greek key $$beta$$-barrel domains; however, a remarkable structural difference was found in the N-terminal region. The unique region has one $$beta$$-strand and one $$alpha$$-helix extended to the northern surface of the type-1 copper site. The superposition of the ${it Geobacillus}$ CuNiR model on the electron-transfer complex structure of CuNiR with the redox partner cytochrome ${it c$_{551}$}$ in other denitrifier system led us to infer that this region contributes to the transient binding with the partner protein during the interprotein electron transfer reaction in the ${it Geobacillus}$ system. Furthermore, electron-transfer kinetics experiments using N-terminal residue-deleted mutant and the redox partner, ${it Geobacillus}$ cytochrome ${it c$_{551}$}$, were carried out. These structural and kinetics studies demonstrate that that region is directly involved in the specific partner recognition.

1 (Records 1-1 displayed on this page)
  • 1