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Journal Articles

Enhancement of plant growth stimulation activity of irradiated alginate by fractionation

Luan, L. Q.*; Nagasawa, Naotsugu; Ha, V. T. T.*; Hien, N. Q.*; Nakanishi, Tomoko*

Radiation Physics and Chemistry, 78(9), p.796 - 799, 2009/09

 Times Cited Count:15 Percentile:68.94(Chemistry, Physical)

Alginate with the weight-average molecular weight ($$M$$$$_{rm w}$$) approximately 900 kDa and ratio of M (mannuronate)/G (guluronate) about 1.3 was irradiated by $$gamma$$ Co-60 in aqueous solution at doses up to 200 kGy. The irradiation dose was shown to be a function for reducing $$M$$$$_{rm w}$$ and molecular weight distribution of irradiated alginates. The distribution of oligomer fractions in irradiated products was also investigated by separation using ultrafiltration membranes. The irradiated alginate with $$M$$$$_{rm w}$$ approximately 14.2 kDa was found to have a positive influence for growing of barley and soybean. The irradiated oligoalginate fraction with $$M$$$$_{rm w}$$ ranging from 1 to 3 kDa displayed the strongest effect on the growth and development of the mentioned plants at low concentration (20 ppm). It is suggested that oligoalginate with $$M$$$$_{rm w}$$ in the range 1-3 kDa is a trigger for the growth and development of plants.

Journal Articles

Enhancement of plant growth activity of irradiated alginate

Nagasawa, Naotsugu; Luan, L. Q.*; Yagi, Toshiaki; Yoshii, Fumio; Kume, Tamikazu; Nakanishi, Tomoko*; Tamada, Masao

JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 55, 2007/02

The sodium alginate was irradiated by $$gamma$$-ray, and plant growth activity test and structural change of the product were studied. It was found that the concentration of 100 mg/L of alginate irradiated at 75 kGy in 4 % aqueous solution was the highest for plant growth. Separation of the degraded product was performed using ultrafiltration membranes and found that fraction with molecular weight in the range of 1-3 kDa (6-15 units) not only showed a remarkable effect on the plant growth, but also increased the activity of plant enzymes; ${it alcohol dehydrogenase, phenylalanine ammonia-lyase}$. This fraction was stronger than one of the enzymatic degradation product. This differences proposed formation of carbonyl and carboxyl groups along with the main-chain scission.

Journal Articles

Enhancement of plant growth activity of irradiated chitosan by molecular weight fractionation

Luan, L. Q.*; Nagasawa, Naotsugu; Tamada, Masao; Nakanishi, Tomoko*

Radioisotopes, 55(1), p.21 - 27, 2006/01

The chitosan products irradiated at 25 - 200 kGy in 10 % solution showed a positive effect on the growth of barley, while the unirradiated chitosan inhibited the growth of this plant. The 100 kGy irradiated chitosan product with average molecular weight (Mw) approx. 16 kDa was found as optimal to plant growth activity. Separation of the degraded samples was performed using ultrafiltaration membranes and was found that the fraction with Mw in range of 1-3 kDa. not only showed a remarkable effect on the growth of barley and soy bean, but also significantly increased the activity of phytoalexin enzymes, namely phenylalanine ammonia lyase (87 %) and chitinase (186 %). This fraction also increased 15.8 % seed yeild of soybean after three month cultivation.

Journal Articles

Biological effect of irradiated chitosan on plants ${it in vitro}$

Luan, L. Q.*; Ha, V. T. T.*; Nagasawa, Naotsugu; Kume, Tamikazu; Yoshii, Fumio; Nakanishi, Tomoko*

Biotechnology and Applied Biochemistry, 41(1), p.49 - 57, 2005/02

no abstracts in English

Journal Articles

Biological effect of radiation-degraded alginate on flower plants in tissue culture

Luan, L. Q.; Hien, N. Q.*; Nagasawa, Naotsugu; Kume, Tamikazu; Yoshii, Fumio; Nakanishi, Tomoko*

Biotechnology and Applied Biochemistry, 38(3), p.283 - 288, 2003/12

 Times Cited Count:36 Percentile:61.75(Biochemistry & Molecular Biology)

no abstracts in English

Journal Articles

Radiation degradation of alginate and some results of biological effect of degraded alginate on plants

Hien, N. Q.*; Hai, L.*; Luan, L. Q.*; Hanh, T. T. V.*; Nagasawa, Naotsugu; Yoshii, Fumio; Makuuchi, Keizo; Kume, Tamikazu

JAERI-Conf 2000-003, p.94 - 100, 2000/03

no abstracts in English

Oral presentation

Enhancement of plant growth activity by irradiated low molecular weight alginate

Nagasawa, Naotsugu; Luan, L. Q.*; Yagi, Toshiaki; Yoshii, Fumio; Kume, Tamikazu; Nakanishi, Tomoko*; Tamada, Masao

no journal, , 

no abstracts in English

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