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Shimada, Mikio*; Tokumiya, Takumi*; Miyake, Tomoko*; Tsukada, Kaima*; Kanzaki, Norie; Yanagihara, Hiromi*; Kobayashi, Junya*; Matsumoto, Yoshihisa*
Journal of Radiation Research (Internet), 64(2), p.345 - 351, 2023/03
Times Cited Count：0 Percentile：0.01(Biology)Noguchi, Masayasu*; Komine, Takashi*; Kamioki, Hiroshi; Matsumoto, Mikio*
Radioisotopes, 50(7), p.301 - 307, 2001/07
no abstracts in English
Matsumoto, Mikio; Endo, Yasuichi; ; Itabashi, Yukio; ; Yokouchi, Iichiro; Ando, Hiroei
JAERI-M 92-212, 62 Pages, 1993/01
no abstracts in English
Endo, Hitoshi; Matsumoto, Atsushi; Kamikubo, Hironari*; Kataoka, Mikio
no journal, ,
Proteins are fundamental for life activities. Their functions highly depend on the structures. However, recent researches suggest that the dynamics of proteins is also important to express the functions. In this study, the structure and dynamics of Staphylococcal nuclease (SNase) in water are investigated by small-angle X-ray scattering and dynamic light scattering. SNase is one of the ideal models to study the dynamics of proteins due to the large fluctuation.
Endo, Hitoshi; Tominaga, Taiki; Takata, Shinichi; Matsumoto, Atsushi; Iwase, Hiroki*; Kamikubo, Hironari*; Kataoka, Mikio
no journal, ,
The dynamic and static structure factors for Staphylococcal Nuclease (SNase), which is a nucleolytic enzyme derived from Staphylococcus aureus, were evaluated by neutron spin echo (NSE)and small-angle neutron scattering (SANS) experiments. The SANS experiment was performed with TAIKAN (BL15) time-of-flight diffractometer at J-PARC/MLF, and we could obtain the static structure factors with wide Q range (0.2 
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2). The NSE measurement was performed with IN15 spectrometer at ILL. Grenoble, which enabled us to obtain intermediate scattering functions over 200 nanoseconds. The effects of hydration and internal motions were considered.
Shimada, Mikio*; Tsukada, Kaima*; Miyake, Tomoko*; Kanzaki, Norie; Yanagihara, Hiromi*; Matsumoto, Yoshihisa*
no journal, ,
Induced pluripotent stem cells (iPSCs) are generated by transduction of reprogramming transcriptional factors. iPSCs have multipotency to differentiate all organs and expected for the application of regenerative medicine. However, it is reported that cancer risk of iPSCs, because of expression of reprogramming factors increased DNA damage. It is important to analysis DNA damage response of iPSCs to prevent chromosomal abnormality and tumor formation. In this study, we attempted to elucidate the molecular mechanism of maintenance of genome stability in iPSCs. RNA-seq analysis by the next generation sequencer showed increased expression of genome maintenance genes such as DNA repair, cell cycle checkpoint and apoptosis. Interestingly, expression level of these genes was decreased after differentiation to the neural stem cells. Furthermore, colony formation assay showed high sensitivity and apoptosis activity to the IR exposure in iPSCs. These results suggested that instead of DNA repair, increasing of apoptosis activity maintain cell population having accurate genome DNA. These molecular insight have important implication for safety medical application of iPSCs.
Shimada, Mikio*; Kanzaki, Norie; Yanagihara, Hiromi*; Miyake, Tomoko*; Matsumoto, Yoshihisa*
no journal, ,
Although mutation frequency depends on organ cell types and differentiation level, it is not fully understood that organ cell types dependent mutation frequency in human cells. In this study, we aimed to establish measurement system of radiation dependent mutation frequency for analyze radiation effect to the human body. For this purpose, we derived four different organ cells such as neural cells, skin keratinocytes, heart muscle cells and blood cells from hiPSCs. Further, using artificial intelligence technology and machine leaning method, we w analyzed differences of mutation frequency during samples.
