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Journal Articles

A Mutation in the ${it uvi4}$ gene promotes progression of endo-reduplication and confers increased tolerance towards ultraviolet B light

Hase, Yoshihiro; Trung, K. H.*; Matsunaga, Tsukasa*; Tanaka, Atsushi

Plant Journal, 46(2), p.317 - 326, 2006/04

 Times Cited Count:86 Percentile:88.84(Plant Sciences)

We have isolated and characterized a new ultraviolet-B (UV-B)-resistant mutant, ${it uvi4}$, of Arabidopsis. The fresh weight of ${it uvi4}$ plants grown under supplemental UV-B light was more than twice that of the wild type. No significant difference was found in the ability to repair the UV-B-induced cyclobutane pyrimidine dimers (CPDs), or in the amount of UV-B absorptive compounds, both of which are well known factors that contribute to UV sensitivity. Positional cloning revealed that the ${it UVI4}$ gene encodes a novel basic protein of unknown function. We found that the hypocotyl cells in ${it uvi4}$ undergo one extra round of endoreduplication. The ${it uvi4}$ mutation also promoted the progression of endoreduplication during leaf development. The ${it UVI4}$ gene is expressed mainly in actively dividing cells. In the leaves of P${it UVI4}$::GUS plants, the GUS signal disappeared in basipetal fashion as the leaf developed. The total leaf blade area was not different between ${it uvi4}$ and the wild type through leaf development, while the average cell area in the adaxial epidermis was considerably larger in ${it uvi4}$, suggesting that the ${it uvi4}$ leaves have fewer but larger epidermal cells. These results suggest that the ${it UVI4}$ is necessary for the maintenance of the mitotic state and the loss of ${it UVI4}$ function stimulated endoreduplication. Tetraploid Arabidopsis was hyperresistant to UV-B compared to diploid Arabidopsis, suggesting that the enhanced polyploidization is responsible for the increased UV-B tolerance of the ${it uvi4}$.

Journal Articles

Characterization of pathways dependent on the ${it uvsE}$, ${it uvrA1}$ or ${it uvrA2}$ gene product for UV resistance in ${it Deinococcus radiodurans}$

Tanaka, Masashi*; Narumi, Issei; Funayama, Tomoo; Kikuchi, Masahiro; Watanabe, Hiroshi*; Matsunaga, Tsukasa*; Nikaido, Osamu*; Yamamoto, Kazuo*

Journal of Bacteriology, 187(11), p.3693 - 3697, 2005/06

 Times Cited Count:34 Percentile:52.97(Microbiology)

no abstracts in English

Journal Articles

Disruption of the ${it AtREV3}$ gene causes hypersensitivity to Ultraviolet B light and $$gamma$$-rays in Arabidopsis; Implication of the presence of a translesion synthesis mechanism in plants

Sakamoto, Ayako; Lan, V. T. T.; Hase, Yoshihiro; Shikazono, Naoya; Matsunaga, Tsukasa*; Tanaka, Atsushi

Plant Cell, 15(9), p.2042 - 2057, 2003/09

 Times Cited Count:80 Percentile:86.11(Biochemistry & Molecular Biology)

In order to investigate UV-response mechanisms in higher plants, we isolated a UV-sensitive mutant, ${it rev3-1}$, in ${it Arabidopsis}$. The root growth of ${it rev3-1}$ was inhibited after UV-B irradiation under both photoreactivating and non-photoreactivating conditions. We found that chromosome 1 of ${it rev3-1}$ was broken at least at three points, causing chromosome inversion and translocation. A gene disrupted by this rearrangement encoded the catalytic subunit of DNA polymerase $$zeta$$ (${it AtREV3}$), which is thought to be involved in translesion synthesis. The ${it rev3-1}$ seedlings were also sensitive to $$gamma$$-rays and MMC, which are known to inhibit DNA replication. The incorporation of BrdU after UV-B irradiation was less in ${it suv1}$ than in the wild type plant. These results suggest that UV-damaged DNA interrupted DNA replication in the ${it rev3-1}$ mutant, leading to the inhibition of cell division and root elongation.

Journal Articles

Amplified UvrA protein can ameliorate the ultraviolet sensitivity of an ${it Escherichia coli recA}$ mutant

Kiyosawa, Kazuhiro*; Tanaka, Masashi*; Matsunaga, Tsukasa*; Nikaido, Osamu*; Yamamoto, Kazuo

Mutation Research; DNA Repair, 487(3-4), p.149 - 156, 2001/12

no abstracts in English

Oral presentation

A Mutation in ${it UVI4}$ gene, which promotes the progression of endoreduplication, confers an increased tolerance against UV-B light

Hase, Yoshihiro; Trung, K. H.*; Matsunaga, Tsukasa*; Tanaka, Atsushi

no journal, , 

We have isolated and characterized an ultraviolet-B resistant mutant, uvi4, of Arabidopsis. The fresh weight of uvi4 plants grown under supplemental UV-B light was more than twice of that of the wild type. No significant difference was found in the ability to repair the UV-B-induced cyclobutane pyrimidine dimmers or in the amount of UV-B absorptive compounds, both of which are well known factors that contribute to UV sensitivity. Positional cloning revealed that the UVI4 gene encodes a novel basic protein of unknown function. The UVI4 gene is expressed mainly in actively dividing cells. We found that the hypocotyls cells in uvi4 undergo one extra round of endoreduplication. The uvi4 mutation also promoted the progression of endoreduplication during leaf development. Tetraploid Arabidopsis was hyperresistant to UV-B than diploid Arabidopsis. These results suggest that the enhanced polyploidization is responsible for the increased UV-B tolerance of the uvi4.

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