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Journal Articles

Track-structure mode for electrons, protons and carbon ions in the PHITS code

Matsuya, Yusuke; Kai, Takeshi; Ogawa, Tatsuhiko; Hirata, Yuho; Sato, Tatsuhiko

Hoshasen Kagaku (Internet), (112), p.15 - 20, 2021/11

Particle and Heavy Ion Transport code System (PHITS) is a general-purpose Monte Carlo code enabling radiation kinetics, which is often used in diverse research fields, such as atomic energy, engineering, medicine and science. After released in 2010, the PHITS code has been developed to expand its functions and to improve its convenience. In the few years, track-structure mode has been introduced in PHITS that can simulate each atomic interaction by electrons, positions, protons and carbon ions in liquid water. Thanks to the development of track-structure mode, the latest PHITS code enables microscopic dose calculations by decomposing it to the scale of DNA. Aiming at realizing the track-structure mode with high precision, the further developments of electron and ion track-structure mode for arbitrary materials are recently ongoing. This review shows the development history and future prospect of PHITS track-structure mode, which can expect to be further applied to the research fields of atomic physics, radiation chemistry, and quantum life science.

Journal Articles

Model development for estimating effects of boron neutron capture therapy

Fukunaga, Hisanori*; Matsuya, Yusuke

Hoshasen Seibutsu Kenkyu, 56(2), p.208 - 223, 2021/06

Boron Neutron Capture Therapy (BNCT) is one of the radiation therapies, enabling selectively eradicating tumors by short-range a-particles and Li ions generated through the nuclear reaction between thermal neutron and $$^{10}$$B within tumor cells. With the development of the accelerator-based neutron source in the recent decades, it is expected that BNCT will be available in many medical facilities worldwide in the future. BNCT irradiation needs a relatively long dose-delivery time after taking up boron drug into tumor cells by intravenous injection. During the period, it is suspected that the boron drug is heterogeneously taken up into cells and its concentration changes continuously, leading to the modification of curative effects from the pharmacological and biological viewpoints. However, the model development for precisely predicting curative effects after BNCT irradiation is still ongoing. Here, we introduce the forefront of model development for estimating the curative effects during BNCT irradiation with high accuracy. This review can create the synergetic effects through an interdisciplinary research approach that can connect the fields of physics, pharmacology, biology and medicine, and would pave the way for new era of BNCT.

Journal Articles

Oxygen enhancement ratios of cancer cells after exposure to intensity modulated X-ray fields; DNA damage and cell survival

Matsuya, Yusuke; McMahon, S. J.*; Butterworth, K. T.*; Naijo, Shingo*; Nara, Isshi*; Yachi, Yoshie*; Saga, Ryo*; Ishikawa, Masayori*; Sato, Tatsuhiko; Date, Hiroyuki*; et al.

Physics in Medicine & Biology, 66(7), p.075014_1 - 075014_11, 2021/04

 Times Cited Count:0 Percentile:0.01(Engineering, Biomedical)

Hypoxic cancer cells within solid tumours show radio-resistance, leading to malignant progression in fractionated radiotherapy. When prescribing dose to tumours under heterogeneous oxygen pressure with intensity-modulated radiation fields, intercellular signalling could have an impact on radiosensitivity between in-field and out-of-field cells. However, the impact of hypoxia on radio-sensitivity under modulated radiation intensity remains uncertain. In this study, we investigate the impact of hypoxia on in-field and out-of-field radio-sensitivities using two types of cancer cells. These in vitro measurements indicate that hypoxia apparently impacts out-of-field cells, although the OER values in out-of-field cells were smaller compared to those for in-field and uniformly irradiated cells. These decreased radio-sensitivities of out-of-field cells were shown as a consistent tendency for both DSB and cell death endpoints, suggesting that radiation-induced intercellular communication is of importance in treatment planning with intensity-modulated radiotherapy.

Journal Articles

4-Methylumbelliferone administration enhances radiosensitivity of human fibrosarcoma by intercellular communication

Saga, Ryo*; Matsuya, Yusuke; Takahashi, Rei*; Hasegawa, Kazuki*; Date, Hiroyuki*; Hosokawa, Yoichiro*

Scientific Reports (Internet), 11(1), p.8258_1 - 8258_10, 2021/04

 Times Cited Count:0 Percentile:0(Multidisciplinary Sciences)

Hyaluronan synthesis inhibitor 4-methylumbelliferone (4-MU) is a candidate of radiosensitizers in X-ray therapy. The curative effects under such 4-MU administration have been investigated in vitro; however, the radiosensitizing mechanisms remain unclear. Here, we investigated the radiosensitizing effects under 4-MU treatment from cell experiments and model estimations. We generated experimental surviving fractions of human fibrosarcoma cells (HT1080) after 4-MU treatment combined with X-ray irradiation. Meanwhilst, we also modelled the pharmacological effects of 4-MU treatment and theoretically analyzed the synergetic effects between 4-MU treatment and X-ray irradiation. The results show that the enhancement of cell killing by 4-MU treatment is the greatest in the intermediate dose range of around 4 Gy, which indicates the involvement of intercellular communication. In addition, the oxidative stress level, which leads to DNA damage induction, significantly increased under 4-MU treatment, and the radiosensitization by 4-MU can be suppressed by the inhibitors for intercellular communication. These findings suggest that the synergetic effects between 4-MU treatment and irradiation are predominantly attributed to intercellular communication and provide more efficient tumour control than conventional X-ray therapy.

Journal Articles

Verification of KURBUC-based ion track structure mode for proton and carbon ions in the PHITS code

Matsuya, Yusuke; Kai, Takeshi; Sato, Tatsuhiko; Liamsuwan, T.*; Sasaki, Kohei*; Nikjoo, H.*

Physics in Medicine & Biology, 66(6), p.06NT02_1 - 06NT02_11, 2021/03

 Times Cited Count:2 Percentile:74.69(Engineering, Biomedical)

A general-purpose Monte Carlo radiation transport simulation code, Particle and Heavy Ion Transport code System (PHITS), has the ability to handle diverse particle types over a wide range of energy. In PHITS version 3.20, ion track structure mode has been developed based on the algorithms in the KURBUC code, which enables to simulate the atomic interactions by primary ion and secondary particles (named as PHITS-KURBUC mode). In this study, we compared the range, radial dose distributions, and microdosimetric distributions calculated using the PHITS-KURBUC mode to the corresponding data obtained from the original KURBUC and from other studies. These comparative studies confirm the successful inclusion of the KURBUC code in the PHITS code. As results of the synergistic effect between the macroscopic and microscopic radiation transport codes, this implementation enabled the detailed calculation of the microdosimetric and nanodosimetric quantities under complex radiation fields, such as proton beam therapy with the spread-out Bragg peak. This PHITS-KURBUC mode is expected to pave the way for next-generation radiation researches, such as radiation physics, radiological protection, medical physics, and radiation biology.

Journal Articles

Development of a new microdosimetric biological weighting function for the RBE$$_{10}$$ assessment in case of the V79 cell line exposed to ions from $$^{1}$$H to $$^{238}$$U

Parisi, A.*; Sato, Tatsuhiko; Matsuya, Yusuke; Kase, Yuki*; Magrin, G.*; Verona, C.*; Tran, L.*; Rosenfeld, A.*; Bianchi, A.*; Olko, P.*; et al.

Physics in Medicine & Biology, 65(23), p.235010_1 - 235010_20, 2020/12

 Times Cited Count:6 Percentile:85.91(Engineering, Biomedical)

A new biological weighting function (IBWF) is proposed to phenomenologically relate microdosimetric lineal energy probability density distributions with the relative biological effectiveness (RBE) for the in vitro clonogenic cell survival (survival fraction = 10%) of the most commonly used mammalian cell line, i.e. the Chinese hamster lung fibroblasts (V79). The RBE values assessed by the IBWF were found to be consistent and in good agreement with the ones calculated in combination with computer-simulated microdosimetric spectra, with an average relative deviation of 0.8% and 5.7% for H and C ions respectively.

Journal Articles

Relation between biomolecular dissociation and energy of secondary electrons generated in liquid water by fast heavy ions

Tsuchida, Hidetsugu*; Kai, Takeshi; Kitajima, Kensei*; Matsuya, Yusuke; Majima, Takuya*; Saito, Manabu*

European Physical Journal D, 74(10), p.212_1 - 212_7, 2020/10

 Times Cited Count:0 Percentile:0.01(Optics)

Fundamental study of interaction between biomolecules and heavy ions in water is very important to predict an initial stage of radiation biological effects. A heavy ion irradiation experiment into droplet target assumed as a biological system in a vacuum was performed to measure production yields of cations and anions for glycine, which was ejected from the droplet target to the vacuum. However, the production mechanisms have been unknown. The PHITS code adapting ion track structure mode was used to analyze the production mechanisms from the dose evaluation at the surface between the vacuum and the water. It is found that induction yields of ionization and excitation, and dissociative electron attachment involved in the secondary electrons were correlated with the production yields of cations and anions of the glycine. The results provide us newly scientific insights to predict an initial stage of radiation biological effects.

Journal Articles

Implications of radiation microdosimetry for accelerator-based boron neutron capture therapy; A Radiobiological perspective

Fukunaga, Hisanori*; Matsuya, Yusuke; Tokuue, Koichi*; Omura, Motoko*

British Journal of Radiology, 93(1111), p.20200311_1 - 20200311_4, 2020/07

 Times Cited Count:0 Percentile:0.02(Radiology, Nuclear Medicine & Medical Imaging)

Boron neutron capture therapy (BNCT) has attracted attention as a selective treatment approach for cancer cells while sparing surrounding normal cells. The basic concept of BNCT was developed in the 1930s, but it has not yet been commonly popular in clinical practice, even though there is now a large number of experimental and translational studies demonstrating its marked therapeutic potential. With the development of neutron accelerators that can be installed in medical institutions, accelerator-based BNCT is expected to become available at several medical institutes around the world in the near future. In this commentary, from the point of view of microdosimetry, we discuss the biological effects of BNCT, especially the underlying mechanisms of cell responses. The recent development of new treatment methods that combine proton beam sources and BNCT technology is expected to contribute significantly improving the prognosis of cancer treatment in the near future. Therefore, radiobiologists in the field of BNCT and related techniques will have a significant role to play in creating synergy effects in clinical oncology.

Journal Articles

A Model for estimating dose-rate effects on cell-killing of human melanoma after boron neutron capture therapy

Matsuya, Yusuke; Fukunaga, Hisanori*; Omura, Motoko*; Date, Hiroyuki*

Cells, 9(5), p.1117_1 - 1117_16, 2020/05

 Times Cited Count:5 Percentile:67.45(Cell Biology)

When delivering a high absorbed dose to cancer cells following boron neutron capture therapy (BNCT), heterogeneous dose distribution, the time line of $$^{10}$$B concentrations and the long dose-delivery time must be considered. Changes in radiosensitivity during such a long dose-delivery time can reduce the probability of tumor control; however, such change has not yet been evaluated. Here, we developed a cell-killing model that accounts for changes in microdosimetric quantities and dose rates depending on the $$^{10}$$B concentration and investigated dose-rate effects (cell recovery during BNCT irradiation) of melanoma. The developed model shows good agreement with in-vitro experimental survival data for exposure to $$^{60}$$Co $$gamma$$-rays, thermal neutrons, and BNCT. The model estimation suggests that the impact of cell recovery during BNCT irradiations with high linear energy transfer (LET) is reduced compared to $$^{60}$$Co $$gamma$$-rays irradiation with low LET. The present model is expected to predict radio-sensitivity for BNCT irradiations.

Journal Articles

A Theoretical cell-killing model to evaluate oxygen enhancement ratios at DNA damage and cell survival endpoints in radiation therapy

Matsuya, Yusuke; Sato, Tatsuhiko; Nakamura, Rui*; Naijo, Shingo*; Date, Hiroyuki*

Physics in Medicine & Biology, 65(9), p.095006_1 - 095006_12, 2020/05

 Times Cited Count:1 Percentile:33.12(Engineering, Biomedical)

Radio-resistance induced under low oxygen pressure plays an important role in malignant progression in fractionated radiotherapy. For the general approach to predict cell killing under hypoxia, cell-killing models (e.g., the Linear-Quadratic model) have to be fitted to ${it in vitro}$ experimental survival data for both normoxia and hypoxia to obtain the oxygen enhancement ratio (OER). However, model parameters for every oxygen condition needs to be considered by model-fitting approaches. This is inefficient for fractionated irradiation planning. Here, we present an efficient model for fractionated radiotherapy the integrated microdosimetric-kinetic model including cell-cycle distribution and the OER at DNA double-strand break endpoint. The cell survival curves described by this model can reproduce the ${it in vitro}$ experimental survival data for both acute and chronic low oxygen concentrations. The OER$$_{DSB}$$ used for calculating cell survival agrees well with experimental DSB ratio of normoxia to hypoxia. This work provides biological effective dose (BED) under various oxygen conditions including its uncertainty, which can contribute to creating fractionated regimens for multi-fractionated radiotherapy. If the oxygen concentration in a tumor can be quantified by medical imaging, the present model will make it possible to estimate the cell-killing and BED under hypoxia in more realistic intravital situations.

Journal Articles

A Simplified Cluster Analysis of Electron Track Structure for Estimating Complex DNA Damage Yields

Matsuya, Yusuke; Nakano, Toshiaki*; Kai, Takeshi; Shikazono, Naoya*; Akamatsu, Ken*; Yoshii, Yuji*; Sato, Tatsuhiko

International Journal of Molecular Sciences (Internet), 21(5), p.1701_1 - 1701_13, 2020/03

 Times Cited Count:4 Percentile:65.16(Biochemistry & Molecular Biology)

Among various DNA damage induced after irradiation, clustered damage composed of at least two vicinal lesions within from 10 to 20 base pairs is recognized as fatal damage to human tissue. Such clustered damage yields have been evaluated by means of computational approaches; however, the simulation validity has not been sufficiently made yet. Meanwhile, the experimental technique to detect clustered DNA damage has been evolved in the recent decades, so both approaches with simulation and experiment get used to be available for investigating clustered damage recently. In this study, we have developed a simple model for estimating clustered damage yield based on the spatial density of ionization and electronic excitation events obtained by the PHITS code, and compared the computational results to the experimental clustered damage coupled with base damage (BD) measured by gel electrophoresis and atomic force microscopy. The computational results agreed well with experimental fractions of clustered damage of strand breaks (SB) and BD, when the yield ratio of BD/SSB is assumed to be 1.3. From the comparison of complex DNA double-strand break coupled with BDs between simulation and experimental data, it was suggested that aggregation degree of the events along electron track reflects the complexity of DNA damage. The resent simulation enables to quantify the type of clustered damage which cannot be measured in in vitro experiment, which succeeded in interpreting the experimental detection efficiency for clustered BD.

Journal Articles

Verification of dose estimation of Auger electrons emitted from Cu-64 using a combination of FNTD measurements and Monte Carlo simulations

Kusumoto, Tamon*; Matsuya, Yusuke; Baba, Kentaro*; Ogawara, Ryo*; Akselrod, M. S.*; Harrison, J.*; Fomenko, V.*; Kai, Takeshi; Ishikawa, Masayori*; Hasegawa, Sumitaka*; et al.

Radiation Measurements, 132, p.106256_1 - 106256_4, 2020/03

 Times Cited Count:0 Percentile:0.01(Nuclear Science & Technology)

Internal radiation therapy with Cu-64 concentrates energy deposition in tumor cells by virtue of released Auger electrons with low energy. In our previous study, we have attached the solutions at the surface of Fluorescent Nuclear Track Detector (FNTD) and succeeded in measuring the absorbed doses of Auger electrons registered in FNTD. However, because there are several types of radiation emitted from the source, i.e., beta rays, positron etc., the contribution degree of Auger electron to energy concentration remain uncertain. In this study, we quantitatively analyzed the spatial dose distribution in the FNTD based on Monte Carlo simulation with PHITS and GEANT4, and evaluated high dose deposited by Auger electrons. The dose distribution calculated by the PHITS code is exactly equivalent to that by Geant4. Also, the simulations are well agreement with experimental results. If the contribution of Auger electrons is ignored, the significantly high absorbed dose proximal to the source is not properly reduced. These findings demonstrate that Auger electrons work very effectively to kill cancer cells proximal to Cu-64 source while minimizing damage effects on normal cells distal to the source.

Journal Articles

Track structure study for energy dependency of electrons and X-rays on DNA double-strand break induction

Yachi, Yoshie*; Yoshii, Yuji*; Matsuya, Yusuke; Mori, Ryosuke*; Oikawa, Joma*; Date, Hiroyuki*

Scientific Reports (Internet), 9(1), p.17649_1 - 17649_8, 2019/11

 Times Cited Count:1 Percentile:15.41(Multidisciplinary Sciences)

Radiation weighting factor for photon and electron is defined as 1.0 independently these energies. However, it should be noted that the biological effects after 29 kVp X-rays is relative higher than standard 200 kVp X-rays at the endpoint of ${it in vitro}$. And it is of importance to evaluate electrons generated via interaction of photons with matter. Here, we evaluated the energy concentration along electron track (dose-mean lineal energy) on chromosome (micron-meter) scales by Monte Carlo simulation, and measured the DNA double-strand breaks (DSBs) induction. From the cell experiments, the DSBs induction after diagnostic X-rays exposure (60-100 kVp) is higher than those with therapeutic X-rays (6 MV). In the relation between the dose-mean lineal energy and the number of DSBs, it was shown that lower energy photons might induce more biological impact due to the interaction by low energy electron. This study implies that radiation weighting factor for photon and electron should not be unity.

Journal Articles

Modeling of yield estimation for DNA strand breaks based on Monte Carlo simulations of electron track structure in liquid water

Matsuya, Yusuke; Kai, Takeshi; Yoshii, Yuji*; Yachi, Yoshie*; Naijo, Shingo*; Date, Hiroyuki*; Sato, Tatsuhiko

Journal of Applied Physics, 126(12), p.124701_1 - 124701_8, 2019/09

 Times Cited Count:10 Percentile:74.82(Physics, Applied)

Biological effects after ionizing radiation exposure arise from initial DNA strand breaks. DNA damage can be estimated from the simulation with both track structure analysis and diffusion of free radicals; however, the simulation is a time-consuming process. In this study, we present a simple model for estimating yields of strand breaks based only on spatial patterns of inelastic interactions (i.e., ionization and electronic excitation) generated by electrons, which are evaluated by PHITS code without considering the production and diffusion of free radicals. In this model, the number of events per track and that of the two events pair within 3.4 nm (corresponding to 10 base pair) were stochastically sampled for calculating SSB and DSB yields, respectively. The calculated results agreed well with other simulations and experimental data on DSB yield and yield ratio of DSB/SSB for the exposure to mono-energetic electrons. The present model also can demonstrate the relative biological effectiveness at the DSB endpoint for various photon exposures. This study indicated that the spatial pattern of inelastic events composed of ionization and electronic excitation is sufficient to obtain the impact of electrons on initial induction to DNA strand break.

Journal Articles

Intensity modulated radiation fields induce protective effects and reduce importance of dose-rate effects

Matsuya, Yusuke; McMahon, S. J.*; Ghita, M.*; Yoshii, Yuji*; Sato, Tatsuhiko; Date, Hiroyuki*; Prise, K. M.*

Scientific Reports (Internet), 9(1), p.9483_1 - 9483_12, 2019/07

 Times Cited Count:6 Percentile:65.54(Multidisciplinary Sciences)

In radiotherapy, intensity modulated radiation fields and complex dose-delivery are used to prescribe doses to tumors. Here, we analyzed the impact of modulated field on radio-sensitivity and cell recovery during irradiation time. The dose was delivered to either 50% of the area of the flask containing cells (half-field) or 100% of the flask (uniform-field). We also modelled cell-killing considering dose-rate effects and intercellular signals. It is found that (i) in-field cell survival under half-field exposure is higher than uniform-field exposure even with the same dose; (ii) the importance of sub-lethal damage repair in normal human skin fibroblast cells under the half-field is reduced; (iii) the increase of cell survival under half-field is predominantly attributed to not rescue effects (increased repair) but protective effects (reduced initial DNA lesion yield). These findings provide new understanding of radio-sensitivity for hit and non-hit cells under non-uniform exposure.

Journal Articles

DNA damage induction during localized chronic exposure to an insoluble radioactive microparticle

Matsuya, Yusuke; Satou, Yukihiko; Hamada, Nobuyuki*; Date, Hiroyuki*; Ishikawa, Masayori*; Sato, Tatsuhiko

Scientific Reports (Internet), 9(1), p.10365_1 - 10365_9, 2019/07

 Times Cited Count:4 Percentile:50.91(Multidisciplinary Sciences)

Insoluble radioactive microparticles (so called Cs-bearing particles) have been assumed to adhere in the long term to trachea after aspirated into respiratory system, leading to heterogeneous dose distribution within healthy tissue around the particles. The biological effects posed by such a particle remain unclear. Here, we show cumulative DNA damage in cultured cells proximal and distal to the particle under localized chronic exposure in comparison with uniform exposure. We placed the particle-contained microcapillary onto a glass-base dish containing normal human lung cells in vitro, and observed a significant change in nuclear $$gamma$$-H2AX foci after 24 h or 48 h exposure to the particle. The dose calculation by a Monte Carlo simulation and the comparison with nuclear foci under uniform exposure suggested that the localized exposure to a Cs-bearing particle leads to not only signal-induced DNA damage to distal cells but also the reduction of DNA damage induction yield to proximal cells (protective effects). Considering the small organ dose, the conventional radiation risk assessment is adequate. This study is the first to quantify the spatial distribution of cumulative DNA lesions under heterogeneous exposure by insoluble Cs-bearing particles.

Journal Articles

Analysis of the high-dose-range radioresistance of prostate cancer cells, including cancer stem cells, based on a stochastic model

Saga, Ryo*; Matsuya, Yusuke; Takahashi, Rei*; Hasegawa, Kazuki*; Date, Hiroyuki*; Hosokawa, Yoichiro*

Journal of Radiation Research, 60(3), p.298 - 307, 2019/05

 Times Cited Count:13 Percentile:87.98(Biology)

In radiotherapy, it is recognized that cancer stem cells (CSCs) in tumor tissue shows radio-resistance. However, the relationship between content percentage of the CSCs and dose-response curve on cell survival remain unclear. In this study, we developed a stochastic model considering progeny cells and stem cells, and investigated the impact of stem cells on radio-sensitivity. From the flow-cytometric analysis (cell experiments), the content percentage of stem cells was 3.2% or less which agreed well with the model estimation from the cell survival curve. Based on the verification, it is suggested that cell survival in high-dose range is largely affected by the CSCs. In addition, regarding the sub-population of stem cells, the present model well reproduces the dose response on lethal lesions to DNA comparing with the conventional LQ model. This outcome indicates that the stem cells must be considered for describing the dose-response curve in wide dose range.

Oral presentation

Estimation of nuclear dose and DNA damage following exposure with cesium bearing microparticles

Matsuya, Yusuke; Hamada, Nobuyuki*; Date, Hiroyuki*; Sato, Tatsuhiko

no journal, , 

After the accident at Fukushima Daiichi Nuclear Power Plant in 2011, insoluble particulate matters with high radio-activity (i.e., cesium bearing microparticles) were found. However, biological effects after the exposure with the microparticles remains unclear. Long-term attachment of the microparticles makes high dose rate around the microparticles. So, it is necessary to perform biological experiments by using cultured cells. For preliminary examining the biological experiments, by using PHITS for dose calculation and mathematical model for DNA damage kinetics (which was developed in Hokkaido University), we estimated the nuclear dose and DNA damage following the exposure with the cesium bearing microparticles so far. As a result, we obtained a finding that DNA damage induced by the microparticles is detectable. In the future, based on this estimation results, we are planning to investigate the biological effects after the exposure with cesium bearing microparticles.

Oral presentation

Modeling for clonogenicity of lens epithelial cells

Oikawa, Joma*; Matsuya, Yusuke; Hamada, Nobuyuki*; Date, Hiroyuki*

no journal, , 

Risk of cataract after exposure has drawn an attention, however its onset mechanisms and the responses to low-dose exposure remain unclear. In recent years, it has been reported that human lens epithelial cells (HLEC1) induce excessive proliferation after exposure with more than 2 Gy, which might be related with cataract onset mechanism. In this study, to clarify the excessive proliferation in HLEC1, a cell colony model was developed by using agent-based model based on NetLogo. This model includes several parameters such as cell cycle, contact inhibition, upper limit of number of cell divisions to reproduce the experimental colony size of non-irradiated cells. From the model study, it was found that excessive proliferation after exposure can be reproduced by considering shortening the cell cycle and increasing the upper limit of cell division. These findings contribute to effective estimation for excessive proliferation of lens epithelial cells and cataract risk after exposure.

Oral presentation

Modeling for colony formation of human lens epithelial cells following ionizing radiation exposure

Oikawa, Joma*; Matsuya, Yusuke; Hamada, Nobuyuki*; Date, Hiroyuki*

no journal, , 

To estimate the risk of radiation cataract following exposure to low dose of ionizing radiation is of importance. However, mechanisms of radiation cataract remain uncertain. Human lens epithelial cells (HLEC1) shows excessive proliferation after exposure above 2 Gy. In this regard, it is necessary for clarifying the relation between the excessive proliferation and the cataract induction. Here, we developed a cell colony model for HLEC1 based on in vitro experiments. We measured cell-cycle and cell growth curve, and then developed the colony model considering cell-cycle time and the limit of cell division number. As the results, we found that shorten the cell cycle time and multiple subpopulation of cells should be considered to reproduce the experimental colony size distribution. The experiments and model estimation suggest that the HLEC1 are heterogeneous population for growth, which may be influential to radiation cataract induction.

34 (Records 1-20 displayed on this page)