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sp. strain V2501 isolated from 250 m below the ground level in Horonobe, Hokkaido, JapanUeno, Akio*; Sato, Kiyoshi*; Tamamura, Shuji*; Murakami, Takuma*; Inomata, Hidenori*; Tamazawa, Satoshi*; Amano, Yuki; Miyakawa, Kazuya; Naganuma, Takeshi*; Igarashi, Toshifumi*
Microbiology Resource Announcements (Internet), 13(11), p.e00108-24_1 - e00108-24_4, 2024/11
and Eu
ions onto sedimentary rock in the presence of gamma-irradiated humic acidZhao, Q.*; Saito, Takeshi*; Miyakawa, Kazuya; Sasamoto, Hiroshi; Kobayashi, Taishi*; Sasaki, Takayuki*
Journal of Hazardous Materials, 428, p.128211_1 - 128211_10, 2022/04
Times Cited Count：6 Percentile：49.04(Engineering, Environmental)The influence of humic acid and its radiological degradation on the sorption of Cs and Eu by sedimentary rock was investigated to understand the sorption process of metal ions and humic substances. Aldrich humic acid (HA) solution was irradiated with different doses of gamma irradiation using a Co-60 gamma-ray source prior to the contact between the metal ions and the solid sorbent. The HA molecule decomposed to smaller molecules with a lower complexation affinity. Batch sorption experiments were performed to evaluate the effect of gamma-irradiated HA on the sorption of Cs and Eu ions. The addition of non-irradiated HA weakened the sorption of Eu because of the lower sorption of the neutral or negatively charged Eu-HA complexes compared with free Eu ions. The sorption of monovalent Cs ions was barely affected by the presence of HA and its gamma irradiation. The concentration ratio of HA complexed species and non-complexed species in the solid and liquid phases was evaluated by sequential filtration and chemical equilibrium calculations. The ratios supported the minimal contribution of HA to Cs sorption. However, the concentration ratio for Eu in the liquid phase was high, indicating that the complexing ability of HA to Eu was higher than that of HA to Cs ions. Therefore, the sorption of free Eu would predominate with the gamma irradiation dose applied to the HA solution under a radiation field near the HLW package.
sp. nov., a mesophilic sulfate-reducing deltaproteobacterium isolated from a deep siliceous mudstone formationUeno, Akio*; Tamazawa, Satoshi*; Tamamura, Shuji*; Murakami, Takuma*; Kiyama, Tamotsu*; Inomata, Hidenori*; Amano, Yuki; Miyakawa, Kazuya; Tamaki, Hideyuki*; Naganuma, Takeshi*; et al.
International Journal of Systematic and Evolutionary Microbiology, 71(2), p.004683_1 - 004683_10, 2021/02
Times Cited Count：10 Percentile：54.37(Microbiology)A novel mesophilic sulfate-reducing bacterium, designated strain HN2
, was isolated from groundwater sampled from the subsurface Miocene Wakkanai Formation located in Horonobe, Hokkaido, Japan. The cells were Gram-negative rods, with motility conferred by a single polar flagellum. The isolate expressed desulfoviridin, but no catalase or oxidase activities was detected. Strain HN2 grew in a temperature range of 5-43 
C (optimum, 35 C) and in a pH range of 6.5-7.5 (optimum, pH 7.0-7.3). It used sulfate, thiosulfate, dimethyl sulfoxide, anthraquinone-2,6-disulfonate, Fe, and manganese oxide as electron acceptors, but not elemental sulfur, nitrite, or nitrate. The bacterium showed very weak growth with sulfite as the electron acceptor. The strain fermented pyruvate and cysteine in the absence of sulfate, but not malate or succinate. The bacterium did not require NaCl, but tolerated up to 4% NaCl (w/v). Strain HN2 did not require vitamins. The G+C content of the genomic DNA was 56.66 mol%. A 16S rRNA gene sequence analysis showed that the closest recognized relative of strain HN2 is 
JS1 (97.0% similarity). The average nucleotide identity (ANI) value between strain HN2 and D. 
JS1 was 79.8%. Based on the phenotypic and molecular genetic evidence, the isolate is assigned to the new species sp. nov. The type strain is HN2 (=DSM 101010 =NBRC 112213).
Hama, Katsuhiro; Sasao, Eiji; Iwatsuki, Teruki; Onoe, Hironori; Sato, Toshinori; Fujita, Tomoo; Sasamoto, Hiroshi; Matsuoka, Toshiyuki; Takeda, Masaki; Aoyagi, Kazuhei; et al.
JAEA-Review 2016-014, 274 Pages, 2016/08
JAEA-Review-2016-014.pdf:44.45MB
We synthesized the research results from the Mizunami/Horonobe Underground Research Laboratories (URLs) and geo-stability projects in the second midterm research phase. This report can be used as a technical basis for the Nuclear Waste Management Organization of Japan/Regulator at each decision point from siting to beginning of disposal (Principal Investigation to Detailed Investigation Phase).
Kaburagi, Masaaki; Yamada, Hironao*; Miyakawa, Takeshi*; Morikawa, Ryota*; Takasu, Masako*; Kato, Takamitsu*; Uesaka, Mitsuru*
Polymer Journal, 48(2), p.189 - 195, 2016/02
Times Cited Count：5 Percentile：17.65(Polymer Science)We performed molecular dynamics (MD) simulations of telomeric single-stranded DNA and POT1 for 100 ns. The distance between 
(POT1) and O5' (telomeric ssDNA) is calculated to verify the binding system for 100 ns MD. We then calculated the distance between the bases of telomeric DNA ends and the root mean square deviation and gyration radius in single and binding states. We compared the root mean square fluctuations between single and binding states and calculated the number of hydrogen bonds between POT1 and telomeric DNA. There are many hydrogen bonds between Gln94 and the first guanine of the closest TTAGGG sequence in telomeric single-stranded DNA. These Gln94 and the guanine have a large difference in root mean square fluctuation between single and binding states. We found that Gln94 and guanine are important components of the binding system, and they are related to its stability.
Tagami, Masahiko*; Yamada, Yasuhiro*; Yamashita, Yoshihiko*; Miyakawa, Ayumu*; Matsuoka, Toshifumi*; Xue, Z.*; Tsuji, Takeshi*; Tsuruta, Tadahiko; Matsuoka, Toshiyuki; Amano, Kenji; et al.
JAEA-Research 2012-036, 110 Pages, 2013/02
JAEA-Research-2012-036.pdf:44.93MB
Northeast strike faults developed in and around the Mizunami Underground Research Laboratory (MIU) control groundwater flow. These faults were possibly formed as a part of pull-apart structure by the right lateral movement of the Tsukiyoshi fault distributed in the north of MIU site. But the formational mechanism of these faults is still uncertain. In this joint research, the analog experiment and the numerical simulation were used to restore the geological structures around MIU site. The paleo-stress analysis were exposed an ancient deformation mechanism, and the formation timing was presumed in the regional tectonics. The results are adopted for the design of the analog experiment and the numerical simulation. The results of obtained analog experiment and numerical simulation are verified three-dimensionally, and then compared to the current geological structure model. Then the geological structure in the uninvestigated area is estimated.
Miyakawa, Shunichi; Yanagisawa, Tsutomu; ; ; Mitsugi, Takeshi
P247, 247 Pages, 2002/00
None
Soga, Tomonori; Tobita, Koichi; Mitsugi, Takeshi; Miyakawa, Shunichi
Saikuru Kiko Giho, (8), p.13 - 22, 2000/09
None
Okamoto, Kaoru; Miyakawa, Shunichi; Mitsugi, Takeshi; Kitamura, Ryoichi
JNC TN9410 99-010, 350 Pages, 1999/06
In the needs of the fuel irradiation test in "Joyo" MK-III core, there have been required that the irradiation of high performance fuel at high liner heat rate to high burn-up range, or the irradiation of advanced fuel such as MA fuel and Vipac fuel. In order to carry out these irradiation tests, newly designed irradiation subassembly is required with special features of; (1)Capability of the re-assembling after post-irradiation examination, even if the number of fuel in the identical irradiation condition decreases because of intermediate inspection. (2)Enhanced flexibility of the irradiation temperature setting ( in the present, UNIS-B's has 6 cases on the maximum). (3)Sufficient flexibility for the coolant flow distribution in the subassembly by extending variety of the flow rate setting. UNIS-D is a fuel irradiation subassembly which has been developed from above viewpoints. It is a compartment loading type irradiation subassembly that is able to load maximum of 18 compartments. Two types of compartments 
-type and 
-typc arc prepared for UNIS-D. Thc sufficient consideration has also been made on the rc-assembling. A -type is the same compartment as the existing UNIS-B's and a -type is the newly designed one for UNIS-D. Three to five fuel pins are loaded into a -type compartment and only one pin is loadcd into a -type compartment. It is possible to carry out the irradiation test in a maximum of 18 test temperature conditions within a subassembly, since it has the sufficient flexibility for the coolant flow distribution. As for the development of UNIS-D, we have finished the detailed structure design and the design verification by the water flow examination, which confirmed that the UNIS-D exceeded its required performances in use and that its structure design was satisfactory.
Soga, Tomonori; Miyakawa, Shunichi; Mitsugi, Takeshi
JNC TN9400 99-052, 355 Pages, 1999/06
Currently, the lifetime of control rods in JOYO is limited by Absorber-Cladding Mechanical Interaction (ACMI) due to swelling of B
C(boron carbide) pellets accelerated by relocation of pellet fragments. A sodium bonded type control rod was developed which improves the thermal conductivity by means of charging sodium into the gap between BC and cladding and by utilizing a shroud which wraps the pellet fragments in a thin tube. This new design will be able to enlarge the gap between BC and cladding, without heating BC or fragment relocation, thus extending the life of the control rod. The sodium bonded type will be fabricated as the ninth reload control rods in JOYO. (1)The specification of a sodium bonded type control rod was determined with the wide gap between BC and cladding. In the design simulation, main component temperature were below the maximum limit. And the local heating by helium bubble generated from BC in the sodium gap, was not a serious problem in the analysis which was considered. (2)A structural design for the sodium entrance into the pin was determined. A formula was developed which the limit for sodium charging given physical dimension of the structure and sodium property. Result from sodium out-pile experiments validated the theoretical formula. (3)The analysis of ACMI indicated a lifetime extension of the sodium bonded type by 4.6% in comparison with lifetime of the helium bonded type of 1.6%. This is due to the boron10 burn-up rate being three times higher in the sodium bonded type than in the helium bonded type. To achieve a target burn-up 10% in the future, it will be necessary to modify design based on irradiation data which will be obtained by practical use of the sodium bonded control rods in JOYO. (4)The effects due to Absorber-Cladding Chemical Interaction (ACCI) were reduced by controlling the cladding temperature and chromium coating to the cladding's inner surface. It was confirmed that ...
Tamazawa, Satoshi*; Ueno, Akio*; Tamaki, Hideyuki*; Tamamura, Shuji*; Murakami, Takuma*; Kiyama, Tamotsu*; Inomata, Hidenori*; Miyakawa, Kazuya; Naganuma, Takeshi*; Kaneko, Katsuhiko*
no journal, ,
no abstracts in English
Ueno, Akio*; Sato, Kiyoshi*; Tamamura, Shuji*; Murakami, Takuma*; Inomata, Hidenori*; Tamazawa, Satoshi*; Amano, Yuki; Miyakawa, Kazuya; Naganuma, Takeshi*; Igarashi, Toshifumi*
no journal, ,
no abstracts in English
Tamazawa, Satoshi*; Ueno, Akio*; Tamaki, Hideyuki*; Tamamura, Shuji*; Murakami, Takuma*; Kiyama, Tamotsu*; Inomata, Hidenori*; Miyakawa, Kazuya; Naganuma, Takeshi*; Kaneko, Katsuhiko*
no journal, ,
no abstracts in English
Tamazawa, Satoshi*; Ueno, Akio*; Murakami, Takuma*; Miyakawa, Kazuya; Tamamura, Shuji*; Kiyama, Tamotsu*; Inomata, Hidenori*; Naganuma, Takeshi*; Kaneko, Katsuhiko*; Igarashi, Toshifumi*
no journal, ,
no abstracts in English
sp. strain Z1-71, isolated from the terrestrial subsurface environment and its co-cultivation within a methanogenic archaeonUeno, Akio*; Tamazawa, Satoshi*; Tamamura, Shuji*; Murakami, Takuma*; Inomata, Hidenori*; Amano, Yuki; Miyakawa, Kazuya; Naganuma, Takeshi*; Igarashi, Toshifumi*
no journal, ,
no abstracts in English
