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Miyazawa, Yutaka*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Negishi, Hiroshi*; Kobayashi, Akie*; Kaneyasu, Tomoko*; Oba, Atsushi*; Morohashi, Keita*; Kakizaki, Takehiko*; et al.
Journal of Radiation Research, 49(4), p.373 - 379, 2008/07
Times Cited Count:29 Percentile:63.75(Biology)We examined the role of root cap and elongation zone cells in root hydrotropism using heavy-ion and laser microbeam. Heavy-ion microbeam irradiation of the elongation zone, but not that of the columella cells, significantly and temporary suppressed the development of hydrotropic curvature. However, laser ablation confirmed that columella cells are indispensable for hydrotropism. Systemic heavy-ion broad-beam irradiation suppressed expression of gene, but not gene. Our results indicate that both the root cap and elongation zone have indispensable and functionally distinct roles in root hydrotropism, and that gene expression might be required for hydrotropism in the elongation zone, but not in columella cells.
Miyazawa, Yutaka*; Sakashita, Tetsuya; Negishi, Hiroshi*; Kobayashi, Akie*; Kaneyasu, Tomoko*; Oba, Atsushi*; Morohashi, Keita*; Kakizaki, Takehiko*; Funayama, Tomoo; Hamada, Nobuyuki*; et al.
JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 116, 2008/03
We examined the role of root cap and elongation zone cells in root hydrotropism of using heavy ion and laser microbeam. Heavy ion microbeam irradiation of the elongation zone, but not that of the columella cells, significantly and temporarily suppressed the development of hydrotropic curvature. However, laser ablation confirmed that columella cells are indispensable for hydrotropism. Systemic heavy ion broad beam irradiation suppressed de novo expression of INDOLE ACETIC ACID 5 gene. Our results indicate that both the root cap and elongation zone have indispensable and functionally distinct roles in root hydrotropism, and that de novo gene expression might be required for hydrotropism in the elongation zone, but not in columella cells.
Storasta, L.*; Tsuchida, Hidekazu*; Miyazawa, Tetsuya*; Oshima, Takeshi
Journal of Applied Physics, 103(1), p.013705_1 - 013705_7, 2008/01
Times Cited Count:95 Percentile:93.19(Physics, Applied)A carbon-implantation/annealing method for annealing of defect Z in thick 4H-SiC epilayers was studied. Different implantation doses and annealing temperatures were examined to find the optimum conditions for annealing of Z. As the result, Z defects in epilayer with 100 m were annealed by implanting 300 nm carbon atoms at a concentration of 210/cm and sbsequent annealing at 1800 C. By this treatment, the carrier lifetime increased from less than 200 ns to over 1 s at room temperature.
Miyazawa, Yutaka*; Negishi, Hiroshi*; Sakashita, Tetsuya; Kobayashi, Akie*; Kaneyasu, Tomoko*; Oba, Atsushi*; Funayama, Tomoo; Wada, Seiichi*; Hamada, Nobuyuki*; Kakizaki, Takehiko; et al.
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 119, 2007/02
Negishi, Hiroshi*; Miyazawa, Yutaka*; Sakashita, Tetsuya; Kobayashi, Akie*; Kaneyasu, Tomoko*; Oba, Atsushi*; Funayama, Tomoo; Wada, Seiichi; Hamada, Nobuyuki; Kakizaki, Takehiko; et al.
no journal, ,
no abstracts in English
Negishi, Hiroshi*; Miyazawa, Yutaka*; Sakashita, Tetsuya; Kobayashi, Akie*; Kaneyasu, Tomoko*; Oba, Atsushi*; Funayama, Tomoo; Wada, Seiichi; Hamada, Nobuyuki; Kakizaki, Takehiko; et al.
no journal, ,
no abstracts in English
Miyazawa, Yutaka*; Negishi, Hiroshi*; Kobayashi, Akie*; Kaneyasu, Tomoko*; Oba, Atsushi*; Fujii, Nobuharu*; Takahashi, Hideyuki*; Sakashita, Tetsuya; Funayama, Tomoo; Wada, Seiichi*; et al.
no journal, ,
no abstracts in English
Miyazawa, Yutaka*; Sakashita, Tetsuya; Negishi, Hiroshi*; Kobayashi, Akie*; Oba, Atsushi*; Kaneyasu, Tomoko*; Morohashi, Keita*; Takahashi, Akiko*; Kakizaki, Takehiko*; Funayama, Tomoo; et al.
no journal, ,
We examined the function of root cap and elongation zone cells in root hydrotropism by nondestructive cell inactivation using heavy-ion microbeam irradiation. Systemic heavy-ion irradiation inactivated de novo indole acetic acid 5 gene expression. Local irradiation of the elongation zone significantly suppressed the development of hydrotropic curvature, whereas local irradiation of the columella cells did not. However, laser ablation confirmed that columella cells are indispensable for hydrotropism. Our results indicate that both the root cap and elongation zone have indispensable and functionally distinct roles in root hydrotropism, and that in the elongation zone, de novo gene expressions, including auxin-responsible gene expression, is required for hydrotropism, whereas in columella cells, it is not.