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Miyazawa, Yutaka*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Negishi, Hiroshi*; Kobayashi, Akie*; Kaneyasu, Tomoko*; Oba, Atsushi*; Morohashi, Keita*; Kakizaki, Takehiko*; et al.
Journal of Radiation Research, 49(4), p.373 - 379, 2008/07
Times Cited Count：28 Percentile：62.78(Biology)We examined the role of root cap and elongation zone cells in root hydrotropism using heavy-ion and laser microbeam. Heavy-ion microbeam irradiation of the elongation zone, but not that of the columella cells, significantly and temporary suppressed the development of hydrotropic curvature. However, laser ablation confirmed that columella cells are indispensable for hydrotropism. Systemic heavy-ion broad-beam irradiation suppressed 
expression of 
gene, but not 
gene. Our results indicate that both the root cap and elongation zone have indispensable and functionally distinct roles in root hydrotropism, and that gene expression might be required for hydrotropism in the elongation zone, but not in columella cells.
using heavy-ion microbeamMiyazawa, Yutaka*; Sakashita, Tetsuya; Negishi, Hiroshi*; Kobayashi, Akie*; Kaneyasu, Tomoko*; Oba, Atsushi*; Morohashi, Keita*; Kakizaki, Takehiko*; Funayama, Tomoo; Hamada, Nobuyuki*; et al.
JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 116, 2008/03
We examined the role of root cap and elongation zone cells in root hydrotropism of using heavy ion and laser microbeam. Heavy ion microbeam irradiation of the elongation zone, but not that of the columella cells, significantly and temporarily suppressed the development of hydrotropic curvature. However, laser ablation confirmed that columella cells are indispensable for hydrotropism. Systemic heavy ion broad beam irradiation suppressed de novo expression of INDOLE ACETIC ACID 5 gene. Our results indicate that both the root cap and elongation zone have indispensable and functionally distinct roles in root hydrotropism, and that de novo gene expression might be required for hydrotropism in the elongation zone, but not in columella cells.
Miyazawa, Yutaka*; Sakashita, Tetsuya; Negishi, Hiroshi*; Kobayashi, Akie*; Oba, Atsushi*; Kaneyasu, Tomoko*; Morohashi, Keita*; Takahashi, Akiko*; Kakizaki, Takehiko*; Funayama, Tomoo; et al.
no journal, ,
We examined the function of root cap and elongation zone cells in root hydrotropism by nondestructive cell inactivation using heavy-ion microbeam irradiation. Systemic heavy-ion irradiation inactivated de novo indole acetic acid 5 gene expression. Local irradiation of the elongation zone significantly suppressed the development of hydrotropic curvature, whereas local irradiation of the columella cells did not. However, laser ablation confirmed that columella cells are indispensable for hydrotropism. Our results indicate that both the root cap and elongation zone have indispensable and functionally distinct roles in root hydrotropism, and that in the elongation zone, de novo gene expressions, including auxin-responsible gene expression, is required for hydrotropism, whereas in columella cells, it is not.
Kato, Shinya; Morohashi, Yuko; Muto, Keitaro
no journal, ,
In order to confirm performance of MONJU failed fuel detection and location system (FFDL), confirmation of accuracy for identifying failed fuel will be conducted by using simulated tag gas.
