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Kawasaki, Riku*; Sasaki, Yoshihiro*; Nishimura, Tomoki*; Katagiri, Kiyofumi*; Morita, Keiichi*; Sekine, Yurina; Sawada, Shinichi*; Mukai, Sadaatsu*; Akiyoshi, Kazunari*
Advanced Healthcare Materials, 10(9), p.2001988_1 - 2001988_8, 2021/05
Times Cited Count:8 Percentile:60(Engineering, Biomedical)Systems for "protein transduction", the intracellular delivery of functional proteins, are needed to address the deliverability challenges of protein therapy, but protein transfer in vivo remains difficult. In this study, we have developed a magnetically induced in vivo protein transfection system using a magnetic nanogel chaperone (MC) composed of iron oxide nanoparticles and polysaccharide nanogels. Experiments using an oral cancer model have shown that this MC system is useful for cancer treatment.
Sekine, Yurina; Endo, Hitoshi*; Iwase, Hiroki*; Takeda, Shigeo*; Mukai, Sadaatsu*; Fukazawa, Hiroshi; Littrell, K. C.*; Sasaki, Yoshihiro*; Akiyoshi, Kazunari*
Journal of Physical Chemistry B, 120(46), p.11996 - 12002, 2016/11
Times Cited Count:10 Percentile:26.96(Chemistry, Physical)The detailed structure of a nanogel formed by self-association of cholesterol-bearing pullulans (CHP) was determined by contrast variation small-angle neutron scattering. The decomposition of scattering intensities into partial scattering functions of each CHP nanogel component, i.e., pullulan, cholesterol, and the cross-term between the pullulan and the cholesterol allows us to investigate the internal structure of the nanogel. The effective spherical radius of the skeleton formed by pullulan chains was found to be about 8.1 nm. In the CHP nanogel, there are about 19 cross-linking points where a cross-linking point is formed by aggregation of trimer cholesterol molecules with the spatially inhomogeneous distribution of the mass fractal dimension of 2.6. The average radius of the partial chains can also be determined to be 1.7 nm. As the result, the complex structure of the nanogels is coherently revealed at the nanoscopical level.
Nagao, Keisuke*; Okazaki, Ryuji*; Nakamura, Tomoki*; Miura, Yayoi*; Osawa, Takahito; Bajo, Kenichi*; Matsuda, Shintaro*; Ebihara, Mitsuru*; Ireland, T.*; Kitajima, Fumio*; et al.
Science, 333(6046), p.1128 - 1131, 2011/08
Times Cited Count:130 Percentile:95.22(Multidisciplinary Sciences)A steroid surface materials record regolith processes and a history of cosmic-ray irradiation. Noble gas isotopes in three rocky grains from Itokawa have been determined. High concentrations of solar He, Ne, and Ar, as high as those in lunar soils, are released at variable temperatures from each sample. The isotopic compositions are essentially identical to those of solar wind but distinguishable in He relative abundance. These noble gas characteristics can be explained by repeated implantation and preferential loss of solar He by removal of weathered He-rich rim on the grain surface through friction among regolith grains on Itokawa. Residence time of regolith materials on Itokawa is alculated to be shorter than 10 Myr, suggesting that regolith materials of small asteroids would escape easily to space.
Yamasaki, Chisato*; Murakami, Katsuhiko*; Fujii, Yasuyuki*; Sato, Yoshiharu*; Harada, Erimi*; Takeda, Junichi*; Taniya, Takayuki*; Sakate, Ryuichi*; Kikugawa, Shingo*; Shimada, Makoto*; et al.
Nucleic Acids Research, 36(Database), p.D793 - D799, 2008/01
Times Cited Count:51 Percentile:71.25(Biochemistry & Molecular Biology)Here we report the new features and improvements in our latest release of the H-Invitational Database, a comprehensive annotation resource for human genes and transcripts. H-InvDB, originally developed as an integrated database of the human transcriptome based on extensive annotation of large sets of fulllength cDNA (FLcDNA) clones, now provides annotation for 120 558 human mRNAs extracted from the International Nucleotide Sequence Databases (INSD), in addition to 54 978 human FLcDNAs, in the latest release H-InvDB. We mapped those human transcripts onto the human genome sequences (NCBI build 36.1) and determined 34 699 human gene clusters, which could define 34 057 protein-coding and 642 non-protein-coding loci; 858 transcribed loci overlapped with predicted pseudogenes.
Sekine, Yurina; Endo, Hitoshi*; Iwase, Hiroki*; Fukazawa, Hiroshi; Mukai, Sadaatsu*; Akiyoshi, Kazunari*
no journal, ,
Cholesterol-bearing pullulan (CHP) spontaneously forms stable nanogel of ca. 30 nm in size in water. Because the CHP nanogels can trap various proteins spontaneously and release them in their native form to environment, they are valuable for use in protein delivery systems, particularly for cancer vaccines, and so on. The distribution and position of cross-linking domain are important factor determining the properties of nanogels. The aim of this study is to investigate the distribution and position of cross-linking domain of nanogels by means of contrast variation small-angle neutron scattering (CV-SANS). We had the CV-SANS measurement of the CHP nanogel dispersed in complex solvent of DO/HO to clarify the microscopic structure of cross-linking domain of CHP nanogels. In this presentation, we will discuss the detailed structure of the CHP nanogel analyzed from the result of CV-SANS.
Sekine, Yurina; Fukazawa, Hiroshi; Endo, Hitoshi*; Iwase, Hiroki*; Mukai, Sadaatsu*; Littrell, K. C.*; Fernandez-Baca, J. A.*; Akiyoshi, Kazunari*
no journal, ,
Cholesterol-bearing pullulan (CHP) spontaneously forms stable nanogel of ca. 30 nm in size in water. Because the CHP nanogels can trap various proteins spontaneously and release them in their native form to environment, they are valuable for use in protein delivery systems. The aim of this study is to investigate the distribution and position of cross-linking domain of nanogels by means of contrast variation small-angle neutron scattering (CV-SANS). We had SANS measurement of the CHP nanogel dispersed in complex solvent of DO/HO to clarify the microscopic structure of cross-linking domain. The result shows that the particle radius of the pullulan and the cholesterol are 8.09 and 6.31 nm, respectively. The dispersion of the cross-linking domains might have important implications for the interactions of nanogels with other molecules.
Sekine, Yurina; Endo, Hitoshi*; Iwase, Hiroki*; Takeda, Shigeo*; Mukai, Sadaatsu*; Fukazawa, Hiroshi; Sasaki, Yoshihiro*; Akiyoshi, Kazunari*
no journal, ,
Cholesterol-bearing pullulan (CHP) forms stable nanogel of 20 nm in size in water. The CHP nanogels can trap various proteins spontaneously. Owing to their characteristics, nanogels can be used in protein delivery systems, particularly for cancer vaccines. The spatial distribution and detailed structure of the cross-linking points are important factor determining their chemical and physical functions. The aim of this study is to investigate the structure of the nanogels by means of contrast variation small-angle neutron scattering (CV-SANS), which enables us to determine quantitative complex structure at nanometer scale. In this presentation, we will discuss the detailed structure of the CHP nanogel.