The Effect of alkyl ammonium ionic liquids on thermal denaturation aggregation of -lactoglobulin

吉田 亨次*; 禪院 知寛*; Fujiyoshi, Ayako*; 真田 雄介*; 山口 敏男*; Murata, Kunihiko*; 高田 慎一; 廣井 孝介; 竹清 貴浩*; 吉村 幸浩*

Journal of Molecular Liquids, 293, p.111477_1 - 111477_9, 2019/11

https://doi.org/10.1016/j.molliq.2019.111477

Differential scanning calorimetry (DSC), small-angle X-ray scattering (SAXS), small-angle neutron scattering (SANS) measurements, and scanning and transmission electron microscope (SEM and TEM) of -lactoglobulin (-LG) are performed in alkyl (methyl, ethyl, and propyl) ammonium nitrate ionic liquids (RAN-ILs)-water mixtures at 25-100C. The addition of these RANs decreased the thermal denaturation temperature of -LG, and the effect is larger with an increase of the hydrophobic part of the cation of the ILs. In ethyl ammonium nitrate (EAN) and propyl ammonium nitrate (PAN) mixtures, the fractal aggregate structure of -LG was found after the thermal denaturation occurred. These results are similar to those of alcohol-water mixtures. The nanoheterogeneous structure of the RAN-water mixture which consists of the hydrophobic and hydrophilic parts might affect the aggregation structure of -LG.