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Takahashi, Akihisa*; Kubo, Makoto*; Ma, H.*; Nakagawa, Akiko*; Yoshida, Yukari*; Isono, Mayu*; Kanai, Tatsuaki*; Ono, Tatsuya*; Furusawa, Yoshiya*; Funayama, Tomoo; et al.
Radiation Research, 182(3), p.338 - 344, 2014/09
Times Cited Count:51 Percentile:90.51(Biology)To clarify whether high-LET radiation inhibits all repair pathways or specifically one repair pathway, studies were designed to examine the effects of radiation with different LET values on DNA DSB repair and radiosensitivity. Embryonic fibroblasts bearing repair gene KO were exposed to X rays, carbon-, iron-, neon- and argon-ion beams. Cell survival was measured with colony-forming assays. The sensitization enhancement ratio (SER) values were calculated using the 10% survival dose of wild-type cells and repair-deficient cells. Cellular radiosensitivity was listed in descending order: double-KO cells NHEJ-KO cells HR-KO cells wild-type cells. Although HR-KO cells had an almost constant SER value, NHEJ-KO cells showed a high-SER value when compared to HR-KO cells, even with increasing LET values. These results suggest that with carbon-ion therapy, targeting NHEJ repair yields higher radiosensitivity than targeting homologous recombination repair.
Nakagawa, Mayu*; Takahashi, Shinya*; Narumi, Issei; Sakamoto, Ayako
Plant Signaling & Behavior (Internet), 6(5), p.728 - 731, 2011/05
Nefissi, R.*; Natsui, Yu*; Miyata, Kana*; Oda, Atsushi*; Hase, Yoshihiro; Nakagawa, Mayu*; Ghorbel, A.*; Mizoguchi, Tsuyoshi*
Journal of Experimental Botany, 62(8), p.2731 - 2744, 2011/02
Times Cited Count:17 Percentile:49.61(Plant Sciences)no abstracts in English
Nakagawa, Mayu*; Takahashi, Shinya*; Tanaka, Atsushi; Narumi, Issei; Sakamoto, Ayako
Plant Physiology, 155(1), p.414 - 420, 2011/01
Times Cited Count:15 Percentile:43.58(Plant Sciences)no abstracts in English
Natsui, Yu*; Nefissi, R.*; Miyata, Kana*; Oda, Atsushi*; Hase, Yoshihiro; Nakagawa, Mayu*; Mizoguchi, Tsuyoshi*
Plant Biotechnology, 27(5), p.463 - 468, 2010/12
Times Cited Count:4 Percentile:15.33(Biotechnology & Applied Microbiology)EARLY FLOWERING 3 (ELF3) is a circadian clock protein with a major role in maintaining circadian rhythms in plants. In this work, was mutagenized by EMS in plants of the Lansberg background to isolate suppressors of and to understand the molecular mechanisms of flowering time controlled by ELF3. Two suppressors, obtained from this screen and from a precious study, were chosen for further analysis. Genetic mapping, gene expression analysis, and sequencing identified and as new alleles of and , respectively. Genetic interactions between and and between and in the control of the floral activator were also investigated. Six suppressor of were classified at least into four subgroups based on the expression of such floral regulators as GI, CO, FT, SVP, and FLC, and on their flowering times under LL, LD, and SD. This classification scheme is useful for the characterization of unidentified suppressor mutations.
Sakamoto, Ayako; Lan, V. T. T.*; Puripunyavanich, V.*; Hase, Yoshihiro; Yokota, Yuichiro; Shikazono, Naoya; Nakagawa, Mayu*; Narumi, Issei; Tanaka, Atsushi
Plant Journal, 60(3), p.509 - 517, 2009/07
Times Cited Count:20 Percentile:50.66(Plant Sciences)no abstracts in English
Takahashi, Shinya*; Nakagawa, Mayu; Tanaka, Atsushi; Narumi, Issei; Shimizu, Kikuo*; Sakamoto, Ayako
JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 58, 2008/11
no abstracts in English
Nefissi, R.*; Miyata, Kana*; Niinuma, Kanae*; Oda, Atsushi*; Hase, Yoshihiro; Nakagawa, Mayu; Mizoguchi, Tsuyoshi*
JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 77, 2008/11
no abstracts in English
Anderson, H.*; Vonarx, E.*; Pastushok, L.*; Nakagawa, Mayu; Katafuchi, Atsushi*; Gruz, P.*; Rubbo, A.*; Grice, D.*; Osmond, M.*; Sakamoto, Ayako; et al.
Plant Journal, 55(6), p.895 - 908, 2008/09
Times Cited Count:42 Percentile:72.7(Plant Sciences)Oda, Atsushi*; Reeves, P. H.*; Tajima, Takeomi*; Nakagawa, Mayu; Kamada, Hiroshi*; Coupland, G.*; Mizoguchi, Tsuyoshi*
Plant Biotechnology, 24(5), p.457 - 465, 2007/12
Times Cited Count:2 Percentile:7.47(Biotechnology & Applied Microbiology)no abstracts in English
Niinuma, Kanae*; Nakagawa, Mayu; Calvino, M.*; Mizoguchi, Tsuyoshi*
Plant Biotechnology, 24(1), p.87 - 97, 2007/03
Times Cited Count:11 Percentile:13.66(Biotechnology & Applied Microbiology)no abstracts in English
Tajima, Takeomi*; Oda, Atsushi*; Nakagawa, Mayu; Kamada, Hiroshi*; Mizoguchi, Tsuyoshi*
Plant Biotechnology, 24(2), p.237 - 240, 2007/03
Times Cited Count:18 Percentile:43.3(Biotechnology & Applied Microbiology)no abstracts in English
Nakagawa, Mayu; Sakamoto, Ayako; Takahashi, Shinya*; Tanaka, Atsushi; Narumi, Issei
no journal, ,
no abstracts in English
Nakagawa, Mayu; Sakamoto, Ayako; Takahashi, Shinya*; Tanaka, Atsushi; Narumi, Issei
no journal, ,
Plants were exposed to the various DNA-damaging stress in daily life. To survive in such hostile environments, plants developed many mechanisms to avoid or to repair the DNA damage. Yeast and mammals have a mechanism named translesion syntheses (TLS), in which DNA damage is bypassed by specific DNA polymerases. However, TLS generates mutations as a result of incorrect replication (error-prone TLS). We have previously isolated AtREV3, AtREV1 and AtREV7 genes, which are homolog of yeast TLS polymerases. We also showed that disruption of these genes made the plants sensitive to UV-B, -ray and MMC. To determine whether AtREV3 acts in the error-prone TLS, we utilized the beta-glucuronidase (uidA) gene containing single nonsense mutations. Transgenic plants with inactivated uidA were treated with UV-C and reversion events were counted. As a result, mutation frequency was reduced in the rev3 background, indicating that AtREV3 acts in the error-prone TLS in Arabidopsis.
Sakamoto, Ayako; Nakagawa, Mayu; Takahashi, Shinya*; Tanaka, Atsushi
no journal, ,
Nakagawa, Mayu; Sakamoto, Ayako; Takahashi, Shinya*; Tanaka, Atsushi; Narumi, Issei
no journal, ,
Nakagawa, Mayu; Sakamoto, Ayako; Takahashi, Shinya*; Tanaka, Atsushi; Narumi, Issei
no journal, ,
no abstracts in English
Sakamoto, Ayako; Takahashi, Shinya*; Nakagawa, Mayu; Tanaka, Atsushi; Shimizu, Kikuo*; Narumi, Issei
no journal, ,
The harmful effects of UVB in the sunlight are unavoidable problems for higher plants that live on photosynthesis. To keep the genomic information intact, plant cells remove the UV damage formed on the DNA by photoreactivation. Plants also have dark-repair and damage tolerance pathways to prevent growth defect caused by DNA damage. Here we report about and genes, which encode specific polymerases to bypass DNA damage. The - or -disrupted plants were more sensitive to UVB, -ray, and DNA crosslink agents than the wild type, suggesting that these REV proteins are required for plant damage tolerance. Although bacterially expressed AtREV1 protein inserted a dCMP at opposite the AP site, it failed to bypass two major UV damages . This inconsistency makes us propose a novel function of AtREV1 in the UV-tolerance pathway. To evaluate the function of AtREV3 and AtREV1 in bypassing UV-damage, we have detected replication errors that often emerged during damage bypass process. The point-mutated, non-functional genes were introduced into Arabidopsis plants and reversion events (mutations) were detected by blue GUS+ sectors on the somatic tissues. We found that a disruption of or reduced the mutation frequency to 1/4 of the level of the wild type. These results suggest that the AtREV3 and AtREV1 are bypassing the DNA damage in error-prone manner.
Sakamoto, Ayako; Nakagawa, Mayu; Sato, Katsuya; Narumi, Issei
no journal, ,
no abstracts in English
Sakamoto, Ayako; Nakagawa, Mayu; Takahashi, Shinya*; Shimizu, Kikuo*; Tanaka, Atsushi; Narumi, Issei
no journal, ,