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Journal Articles

Nonhomologous end-joining repair plays a more important role than homologous recombination repair in defining radiosensitivity after exposure to high-LET radiation

Takahashi, Akihisa*; Kubo, Makoto*; Ma, H.*; Nakagawa, Akiko*; Yoshida, Yukari*; Isono, Mayu*; Kanai, Tatsuaki*; Ono, Tatsuya*; Furusawa, Yoshiya*; Funayama, Tomoo; et al.

Radiation Research, 182(3), p.338 - 344, 2014/09

 Times Cited Count:51 Percentile:90.51(Biology)

To clarify whether high-LET radiation inhibits all repair pathways or specifically one repair pathway, studies were designed to examine the effects of radiation with different LET values on DNA DSB repair and radiosensitivity. Embryonic fibroblasts bearing repair gene KO were exposed to X rays, carbon-, iron-, neon- and argon-ion beams. Cell survival was measured with colony-forming assays. The sensitization enhancement ratio (SER) values were calculated using the 10% survival dose of wild-type cells and repair-deficient cells. Cellular radiosensitivity was listed in descending order: double-KO cells $$>$$ NHEJ-KO cells $$>$$ HR-KO cells $$>$$ wild-type cells. Although HR-KO cells had an almost constant SER value, NHEJ-KO cells showed a high-SER value when compared to HR-KO cells, even with increasing LET values. These results suggest that with carbon-ion therapy, targeting NHEJ repair yields higher radiosensitivity than targeting homologous recombination repair.

Journal Articles

Role of AtPol$$zeta$$, AtRev1 and AtPol$$eta$$ in $$gamma$$-ray-induced mutagenesis

Nakagawa, Mayu*; Takahashi, Shinya*; Narumi, Issei; Sakamoto, Ayako

Plant Signaling & Behavior (Internet), 6(5), p.728 - 731, 2011/05

Journal Articles

Double loss-of-function mutation in ${it EARLY FLOWERING 3}$ and ${it CRYPTOCHROME 2}$ genes delays flowering under continuous light but accelerates it under long days and short days; An Important role for ${it Arabidopsis}$ CRY2 to accelerate flowering time in continuous light

Nefissi, R.*; Natsui, Yu*; Miyata, Kana*; Oda, Atsushi*; Hase, Yoshihiro; Nakagawa, Mayu*; Ghorbel, A.*; Mizoguchi, Tsuyoshi*

Journal of Experimental Botany, 62(8), p.2731 - 2744, 2011/02

 Times Cited Count:17 Percentile:49.61(Plant Sciences)

no abstracts in English

Journal Articles

Role of AtPol$$zeta$$, AtRev1 and AtPol$$eta$$ in UV light-induced mutagenesis in Arabidopsis$$^{1[W]}$$

Nakagawa, Mayu*; Takahashi, Shinya*; Tanaka, Atsushi; Narumi, Issei; Sakamoto, Ayako

Plant Physiology, 155(1), p.414 - 420, 2011/01

 Times Cited Count:15 Percentile:43.58(Plant Sciences)

no abstracts in English

Journal Articles

Isolation and characterization of suppressors of the ${it early flowering 3}$ in ${it Arabidopsis thaliana}$

Natsui, Yu*; Nefissi, R.*; Miyata, Kana*; Oda, Atsushi*; Hase, Yoshihiro; Nakagawa, Mayu*; Mizoguchi, Tsuyoshi*

Plant Biotechnology, 27(5), p.463 - 468, 2010/12

 Times Cited Count:4 Percentile:15.33(Biotechnology & Applied Microbiology)

EARLY FLOWERING 3 (ELF3) is a circadian clock protein with a major role in maintaining circadian rhythms in plants. In this work, ${it elf3-101}$ was mutagenized by EMS in plants of the Lansberg ${it erecta}$ background to isolate suppressors of ${it elf3}$ and to understand the molecular mechanisms of flowering time controlled by ELF3. Two suppressors, ${it sel106}$ obtained from this screen and ${it sel5}$ from a precious study, were chosen for further analysis. Genetic mapping, gene expression analysis, and sequencing identified ${it sel106}$ and ${it sel5}$ as new alleles of ${it gi}$ and ${it fca}$, respectively. Genetic interactions between ${it elf3}$ and ${it gi}$ and between ${it elf3}$ and ${it fca}$ in the control of the floral activator ${it FLOWERING LOCUS T}$ were also investigated. Six suppressor of ${it elf3}$ were classified at least into four subgroups based on the expression of such floral regulators as GI, CO, FT, SVP, and FLC, and on their flowering times under LL, LD, and SD. This classification scheme is useful for the characterization of unidentified suppressor mutations.

Journal Articles

A UVB-hypersensitive mutant in ${it Arabidopsis thaliana}$ is defective in the DNA damage response

Sakamoto, Ayako; Lan, V. T. T.*; Puripunyavanich, V.*; Hase, Yoshihiro; Yokota, Yuichiro; Shikazono, Naoya; Nakagawa, Mayu*; Narumi, Issei; Tanaka, Atsushi

Plant Journal, 60(3), p.509 - 517, 2009/07

 Times Cited Count:20 Percentile:50.66(Plant Sciences)

no abstracts in English

Journal Articles

Functional analysis of the low-fidelity DNA polymerase AtREV1

Takahashi, Shinya*; Nakagawa, Mayu; Tanaka, Atsushi; Narumi, Issei; Shimizu, Kikuo*; Sakamoto, Ayako

JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 58, 2008/11

no abstracts in English

Journal Articles

Characterization of enhancers and suppressors of circadian clock mutant ${it elf3}$ in Arabidopsis

Nefissi, R.*; Miyata, Kana*; Niinuma, Kanae*; Oda, Atsushi*; Hase, Yoshihiro; Nakagawa, Mayu; Mizoguchi, Tsuyoshi*

JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 77, 2008/11

no abstracts in English

Journal Articles

${it Arabidopsis thaliana}$ Y-family DNA polymerase $$eta$$ catalyses translesion synthesis and interacts functionally with PCNA2

Anderson, H.*; Vonarx, E.*; Pastushok, L.*; Nakagawa, Mayu; Katafuchi, Atsushi*; Gruz, P.*; Rubbo, A.*; Grice, D.*; Osmond, M.*; Sakamoto, Ayako; et al.

Plant Journal, 55(6), p.895 - 908, 2008/09

 Times Cited Count:42 Percentile:72.7(Plant Sciences)

Journal Articles

Isolation of novel gain- and loss-of-function alleles of the circadian clock gene ${it LATE ELONGATED HYPOCOTYL}$ (${it LHY}$) in ${it Arabidopsis}$

Oda, Atsushi*; Reeves, P. H.*; Tajima, Takeomi*; Nakagawa, Mayu; Kamada, Hiroshi*; Coupland, G.*; Mizoguchi, Tsuyoshi*

Plant Biotechnology, 24(5), p.457 - 465, 2007/12

 Times Cited Count:2 Percentile:7.47(Biotechnology & Applied Microbiology)

no abstracts in English

Journal Articles

Dance of plants with circadian clock

Niinuma, Kanae*; Nakagawa, Mayu; Calvino, M.*; Mizoguchi, Tsuyoshi*

Plant Biotechnology, 24(1), p.87 - 97, 2007/03

 Times Cited Count:11 Percentile:13.66(Biotechnology & Applied Microbiology)

no abstracts in English

Journal Articles

Natural variation of polyglutamine repeats of a circadian clock gene ${it ELF3}$ in ${it Arabidopsis}$

Tajima, Takeomi*; Oda, Atsushi*; Nakagawa, Mayu; Kamada, Hiroshi*; Mizoguchi, Tsuyoshi*

Plant Biotechnology, 24(2), p.237 - 240, 2007/03

 Times Cited Count:18 Percentile:43.3(Biotechnology & Applied Microbiology)

no abstracts in English

Oral presentation

Analysis of TLS related factors in Arabidopsis

Nakagawa, Mayu; Sakamoto, Ayako; Takahashi, Shinya*; Tanaka, Atsushi; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

AtREV3 induces mutations in Arabidopsis

Nakagawa, Mayu; Sakamoto, Ayako; Takahashi, Shinya*; Tanaka, Atsushi; Narumi, Issei

no journal, , 

Plants were exposed to the various DNA-damaging stress in daily life. To survive in such hostile environments, plants developed many mechanisms to avoid or to repair the DNA damage. Yeast and mammals have a mechanism named translesion syntheses (TLS), in which DNA damage is bypassed by specific DNA polymerases. However, TLS generates mutations as a result of incorrect replication (error-prone TLS). We have previously isolated AtREV3, AtREV1 and AtREV7 genes, which are homolog of yeast TLS polymerases. We also showed that disruption of these genes made the plants sensitive to UV-B, $$gamma$$-ray and MMC. To determine whether AtREV3 acts in the error-prone TLS, we utilized the beta-glucuronidase (uidA) gene containing single nonsense mutations. Transgenic plants with inactivated uidA were treated with UV-C and reversion events were counted. As a result, mutation frequency was reduced in the rev3 background, indicating that AtREV3 acts in the error-prone TLS in Arabidopsis.

Oral presentation

Functional analysis of DNA polymerase zeta and REV1 protein in Arabidopsis

Sakamoto, Ayako; Nakagawa, Mayu; Takahashi, Shinya*; Tanaka, Atsushi

no journal, , 

Oral presentation

Does Arabidopsis have translesion syntheses ?

Nakagawa, Mayu; Sakamoto, Ayako; Takahashi, Shinya*; Tanaka, Atsushi; Narumi, Issei

no journal, , 

Oral presentation

Analysis of mutation frequency in Arabidopsis REV3 and REV1 deficient mutants

Nakagawa, Mayu; Sakamoto, Ayako; Takahashi, Shinya*; Tanaka, Atsushi; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Translesion synthesis and UV-sensitivity in higher plants

Sakamoto, Ayako; Takahashi, Shinya*; Nakagawa, Mayu; Tanaka, Atsushi; Shimizu, Kikuo*; Narumi, Issei

no journal, , 

The harmful effects of UVB in the sunlight are unavoidable problems for higher plants that live on photosynthesis. To keep the genomic information intact, plant cells remove the UV damage formed on the DNA by photoreactivation. Plants also have dark-repair and damage tolerance pathways to prevent growth defect caused by DNA damage. Here we report about ${it AtREV3}$ and ${it AtREV1}$ genes, which encode specific polymerases to bypass DNA damage. The ${it AtREV3}$- or ${it AtREV1}$-disrupted plants were more sensitive to UVB, $$gamma$$-ray, and DNA crosslink agents than the wild type, suggesting that these REV proteins are required for plant damage tolerance. Although bacterially expressed AtREV1 protein inserted a dCMP at opposite the AP site, it failed to bypass two major UV damages ${it in vitro}$. This inconsistency makes us propose a novel function of AtREV1 in the UV-tolerance pathway. To evaluate the function of AtREV3 and AtREV1 in bypassing UV-damage, we have detected replication errors that often emerged during damage bypass process. The point-mutated, non-functional ${it uidA}$ genes were introduced into Arabidopsis plants and reversion events (mutations) were detected by blue GUS+ sectors on the somatic tissues. We found that a disruption of ${it AtREV3}$ or ${it AtREV1}$ reduced the mutation frequency to 1/4 of the level of the wild type. These results suggest that the AtREV3 and AtREV1 are bypassing the DNA damage in error-prone manner.

Oral presentation

${it AtRad26}$ is involved in the damage checkpoint in Arabidopsis

Sakamoto, Ayako; Nakagawa, Mayu; Sato, Katsuya; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Translesion synthesis and mutagenesis in higher plants

Sakamoto, Ayako; Nakagawa, Mayu; Takahashi, Shinya*; Shimizu, Kikuo*; Tanaka, Atsushi; Narumi, Issei

no journal, , 

25 (Records 1-20 displayed on this page)