Effect of nanoscale cellular structure on the mechanical properties of Inconel 718 with unique hierarchical structure fabricated by laser powder bed fusion

Cho, K.*; Yamashita, Kippei*; Kakutani, Shinnosuke*; Saito, Takuma*; Sasaki, Taisuke*; Sawaizumi, Katsuhiko*; Okugawa, Masayuki*; Koizumi, Yuichiro*; Mayama, Tsuyoshi*; Kikukawa, Taichi*; et al.

Acta Materialia, 303, p.121696_1 - 121696_18, 2026/01

https://doi.org/10.1016/j.actamat.2025.121696

Energetic heavy ions overcome tumor radioresistance caused by overexpression of Bcl-2

Hamada, Nobuyuki*; Hara, Takamitsu*; Omura, Motoko*; Funayama, Tomoo; Sakashita, Tetsuya; Sora, Sakura; Yokota, Yuichiro; Nakano, Takashi*; Kobayashi, Yasuhiko

Radiotherapy and Oncology, 89(2), p.231 - 236, 2008/11

https://doi.org/10.1016/j.radonc.2008.02.013

Patterning irradiation for contact co-culture of different type cells using heavy-ion microbeam

Ikeda, Hiroko; Yokota, Yuichiro; Funayama, Tomoo; Kanai, Tatsuaki*; Nakano, Takashi*; Kobayashi, Yasuhiko

no journal, , 

There have been some reports on bystander effects induced by proton microbeam in contact co-cultured different type cells, but there are few reports using heavy-ion microbeam. So, we have established a new contact co-culture system between human lung normal fibroblast cell line WI-38 and human lung cancer cell line H1299/wt in the same dish. We have also adapted patterning irradiation systems which automatically irradiated to cancer cells (or normal cells) in a certain range by making use of the target cell irradiation technique at JAEA-Takasaki. Thereby, we were able to successively irradiate to 250 sites of confluent cancer area (lengthwise: 5 mm) so as not to overlap the irradiated range, using carbon-ion microbeam collimated by aperture of 20 m. Now, we are analyzing DNA damage and repair of patterning irradiation samples by evaluating focus numbers of immunostained 53BP1 and -H2AX. The details of the method and findings will be reported in the talk.

Analysis of dose-response in bystander effects between human lung normal and cancer cells

Ikeda, Hiroko; Yokota, Yuichiro; Funayama, Tomoo; Kanai, Tatsuaki*; Nakano, Takashi*; Kobayashi, Yasuhiko

no journal, , 

Human lung normal fibroblasts WI-38 and human lung cancer cells H1299/wt were used. Cells were irradiated with carbon-ion broad beams (LET=108 keV/ m), then survival rates of bystander cells after co-culture with irradiated cells were measured using colony formation assay. The survival rates of non-irradiated H1299/wt cells co-cultured with 0.13 Gy irradiated WI-38 increased after 6 and 24 h of co-culture. On the other hand, the bystander cells co-cultured with 0.5 Gy irradiated WI-38 showed decreased survival rates. The survival rates of bystander H1299/wt cells showed a tendency to increase by the addition of Carboxy-PTIO to the co-culture medium, when co-cultured with 0.5 Gy irradiated WI-38. From these results, reduction of survival rates is likely to be caused by NO radical as a mediator in bystander effects between lung normal and cancer cells. However, it is suggested that there might be other signals participated in an increase of survival rates.