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Journal Articles

Crossbreeding of a metallic color carnation and diversification of the peculiar coloration by ion-beam irradiation

Okamura, Masachika*; Nakayama, Masayoshi*; Umemoto, Naoyuki*; Cano, E. A.*; Hase, Yoshihiro; Nishizaki, Yuzo*; Sasaki, Nobuhiro*; Ozeki, Yoshihiro*

Euphytica, 191(1), p.45 - 56, 2013/05

 Times Cited Count:17 Percentile:73.52(Agronomy)

A few carnation cultivars are known to display a peculiar dusky color supposedly caused by anthocyanic vacuolar inclusions (AVIs). The hereditary pattern suggests that the peculiar color is controlled by a single recessive factor tightly linked with existence of AVIs containing non-acylated anthocyanins. To diversify the peculiar color carnation, we produced a bluish purple line displaying a highly novel metallic appearance by crossbreeding. By subjecting the line to ion-beam irradiation, we generated metallic reddish purple, metallic crimson and metallic red lines. All four metallic lines did not have transcripts for anthocyanin malyltransferase. In contrast to the dusky color types, metallic lines have highly condensed AVIs and water-clear vacuolar sap in the petal adaxial epidermal cells. We demonstrated that (1) a factor generating the AVIs is inactivated anthocyanin malyltransferase gene, (2) AVIs in water-clear vacuolar sap in the adaxial epidermal cells generate the novel metallic appearance, and (3) ion beam breeding is a useful tool for increasing metallic colors by changing anthocyanin structure and the level of AVIs.

Journal Articles

Isolation and characterization of the fragrant cyclamen ${it O}$-methyltransferase involved in flower coloration

Akita, Yusuke; Kitamura, Satoshi; Hase, Yoshihiro; Narumi, Issei; Ishizaka, Hiroshi*; Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Tanikawa, Natsu*; Morita, Yasumasa*; et al.

Planta, 234(6), p.1127 - 1136, 2011/12

 Times Cited Count:22 Percentile:62.41(Plant Sciences)

Journal Articles

Red-purple flower due to delphinidin 3,5-diglucoside, a novel pigment for ${it Cyclamen}$ spp., generated by ion-beam irraidation

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 65, 2011/01

no abstracts in English

Journal Articles

Comparative analysis of floral pigmentation between wild-type and white-flowered varieties of ${it Cyclamen graecum}$

Akita, Yusuke; Ishizaka, Hiroshi*; Nakayama, Masayoshi*; Shimada, Akihiko; Kitamura, Satoshi; Hase, Yoshihiro; Narumi, Issei; Tanaka, Atsushi

Journal of Horticultural Science & Biotechnology, 85(5), p.437 - 443, 2010/09

The flower colour of ${it Cyclamen graecum}$ gra6 (wild-type) is pink-purple in the flower. On the other hand, flowers of ${it C. graecum}$ gra50 (white-flowered variant) exhibit a white colour in its flower. In this study, the relationship between floral pigmentation and expression of several anthocyanin biosynthesis genes was investigated in ${it C. graecum}$ gra6 and gra50. The pigments in the flower consist mainly of malvidin 3,5-diglucoside in gra6. White-flowered ${it C. graecum}$ gra50 possessed lower amounts of anthocyanins, but higher amounts of flavonols compared to gra6. Gene expression analysis demonstrated that expression of the dihydroflavonol 4-reductase gene 2 (${it CgraDFR2}$) was lower in gra50 compared with gra6, whereas the expression of other key genes did not differ greatly. These results suggest that the white-flowered variant of gra50 may result from a defect in expression of the ${it CgraDFR2}$ gene.

Journal Articles

Red-purple flower due to delphinidin 3,5-diglucosides, a novel pigment for ${it Cyclamen}$ spp., generated by ion-beam irradiation

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

Plant Biotechnology, 26(5), p.565 - 569, 2009/01

 Times Cited Count:27 Percentile:65.26(Biotechnology & Applied Microbiology)

Fragrant cyclamen cultivar (${it C. persicum}$ $$times$$ ${it C. purpurascens}$), that bloomed purple flower containing malvidin 3,5-diglucoside as the major anthocyanin, was irradiated with a 320-MeV carbon-ion beam at 0-16 Gy to increase flower color variation by mutation. Some of the M2 plants derived from self-pollination of M1 plants irradiated at 2 Gy were flower-color mutants that retained desirable flower shape, flower size, and leaf color. One of the mutants bloomed novel red-purple flowers, the major anthocyanin of which was delphinidin 3,5-diglucoside. Because the major anthocyanins in flowers of ${it Cyclamen}$ spp. were previously restricted to malvidin, peonidin, and cyanidin types, the generation of a cyclamen containing mostly the delphinidin-type anthocyanin is an important breakthrough in cyclamen breeding. We expect this mutant to become not only a commercial cultivar itself, but also a valuable genetic resource for cyclamen breeding.

JAEA Reports

Development and management of the knowledge base for the geological disposal technology; Annual report 2006

Umeda, Koji; Oi, Takao; Osawa, Hideaki; Oyama, Takuya; Oda, Chie; Kamei, Gento; Kuji, Masayoshi*; Kurosawa, Hideki; Kobayashi, Yasushi; Sasaki, Yasuo; et al.

JAEA-Review 2007-050, 82 Pages, 2007/12

JAEA-Review-2007-050.pdf:28.56MB

This report shows the annual report which shows the summarized results and topic outline of each project on geological disposal technology in the fiscal year of 2006.

Journal Articles

t+$$^{3}$$He molecular structures in $$^{6}$$Li studied ($$^{3}$$He,$$alpha$$) reaction at 450 MeV

Nakayama, Shintaro*; Yamagata, Tamio*; Akimune, Hidetoshi*; Fujiwara, Mamoru; Fushimi, Kenichi*; Greenfield, M. B.*; Hara, Keigo*; Hara, Kaoru*; Hashimoto, Hisanobu*; Ichihara, Kayoko*; et al.

Nuclear Physics A, 731, p.355 - 362, 2004/02

 Times Cited Count:0 Percentile:0.01(Physics, Nuclear)

no abstracts in English

Journal Articles

Di-triton molecular structure in $$^{6}$$He

Akimune, Hidetoshi*; Yamagata, Tamio*; Nakayama, Shintaro*; Arimoto, Yasushi*; Fujiwara, Mamoru; Fushimi, Kenichi*; Hara, Keigo*; Ota, Masahisa*; Shiokawa, Atsuko*; Tanaka, Masayoshi*; et al.

Physical Review C, 67(5), p.051302_1 - 051302_4, 2003/05

 Times Cited Count:26 Percentile:79.94(Physics, Nuclear)

no abstracts in English

Journal Articles

Issues to be verified by IFMIF prototype accelerator for engineering validation

Sugimoto, Masayoshi; Imai, Tsuyoshi; Okumura, Yoshikazu; Nakayama, Koichi*; Suzuki, Shohei*; Saigusa, Mikio*

Journal of Nuclear Materials, 307-311(Part2), p.1691 - 1695, 2002/12

 Times Cited Count:2 Percentile:17.59(Materials Science, Multidisciplinary)

International Fusion Materials Irradiation Facility (IFMIF) is an accelerator-based intense neutron source for fusion reactor materials development. Each one of the two accelerator modules needs to have a capability to provide the 40MeV/125mA deuteron beam continuously. Although the technology to produce the 7MeV/100mA proton beam is already verified using 350 MHz linac in the past, an engineering study using a prototype is necessary to verify the performance of IFMIF 175 MHz deuteron linac, and Engineering Validation Phase (EVP) is planned for this purpose. Some critical design parameters, like final and transition energies of linacs or RF source characteristics, are needed to be optimised for the prototype. As it is also important to verify the essential component technology, e.g. ion source, RFQ beam matching, rf system components, etc., the present status and expected results of such undergoing verification tests are described. An integrated concept of prototype accelerator is shown as a Japanese proposal for EVP to provide for the international discussion.

Journal Articles

Dipole excitation of $$alpha$$ clusters in $$^{6}$$Li and $$^{7}$$Li

Nakayama, Shintaro*; Yamagata, Tamio*; Akimune, Hidetoshi*; Daito, Izuru*; Fujimura, Hisako*; Fujita, Yoshitaka*; Fujiwara, Mamoru; Fushimi, Kenichi*; Greenfield, M. B.*; Kori, Hideki*; et al.

Physical Review Letters, 87(12), p.122502_1 - 122502_4, 2001/09

 Times Cited Count:20 Percentile:70(Physics, Multidisciplinary)

no abstracts in English

Journal Articles

Soft dipole resonance in the neutron-skin nucleus $$^{6}$$He

Nakayama, Shintaro*; Yamagata, Tamio*; Akimune, Hidetoshi*; Daito, Izuru*; Fujimura, Hisako*; Fujita, Yoshitaka*; Fujiwara, Mamoru; Fushimi, Kenichi*; Inomata, Toru*; Kori, Hideki*; et al.

Physical Review Letters, 85(2), p.262 - 265, 2000/07

 Times Cited Count:72 Percentile:89.44(Physics, Multidisciplinary)

no abstracts in English

Journal Articles

Excitation of isovector spin-dipole resonances and neutron skin of nuclei

Krasznahorkay, A.*; Fujiwara, Mamoru; Van Aarle, P.*; Akimune, Hidetoshi; Daito, Izuru*; Fujimura, Hisako*; Fujita, Yoshitaka*; Harakeh, M. N.*; Inomata, Toru*; J$"a$necke, J.*; et al.

Physical Review Letters, 82(16), p.3216 - 3219, 1999/04

 Times Cited Count:168 Percentile:96.39(Physics, Multidisciplinary)

no abstracts in English

Oral presentation

Comparative analysis of floral pigmentation between Cyclamen graecum and its colorless mutant

Akita, Yusuke; Ishizaka, Hiroshi*; Shimada, Akihiko; Nakayama, Masayoshi*; Kitamura, Satoshi; Hase, Yoshihiro; Tanaka, Atsushi; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Mutation induction in fragrance cyclamen (${it Cyclamen persicum}$ x ${it C. purpurascens}$) by ion beam re-irradiation

Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Akita, Yusuke; Hase, Yoshihiro; Tanikawa, Natsu*; Morita, Yasumasa*; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Comparative analysis of flavonoid biosynthesis genes between ${it Cyclamen graecum}$ and its white-flowered mutant

Akita, Yusuke; Ishizaka, Hiroshi*; Nakayama, Masayoshi*; Shimada, Akihiko; Kitamura, Satoshi; Hase, Yoshihiro; Tanaka, Atsushi; Narumi, Issei

no journal, , 

Oral presentation

Analysis of flower pigmentation genes in cyclamen

Akita, Yusuke; Ishizaka, Hiroshi*; Nakayama, Masayoshi*; Kitamura, Satoshi; Hase, Yoshihiro; Tanaka, Atsushi; Narumi, Issei

no journal, , 

no abstracts in English

Oral presentation

Induction of flower color mutation in fragrance cyclamen, "Kaori-no-mai" by ion beam irradiation

Kondo, Emiko*; Nakayama, Masayoshi*; Kameari, Naoko*; Tanikawa, Natsu*; Morita, Yasumasa*; Akita, Yusuke; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

One of the fragrant cyclamens, Kaori-no-mai, blooms purple flowers containing malvidin 3,5-diglucoside as the major anthocyanin. Here, we irradiated etiolated petioles of Kaori-no-mai with a 320-MeV carbon-ion beam to increase flower color variation by mutation. Some of the M2 plants derived from self-pollination of M1 plants were flower-color mutan. One of the mutants bloomed novel red-purple flowers, the major anthocyanin of which was delphinidin 3,5-diglucoside. The major anthocyanins in flowers of ${it Cyclamen }$ spp. were previously restricted to malvidin, peonidin, and cyanidin types, therefore, the generation of a cyclamen containing mostly the delphinidin-type anthocyanin is an important breakthrough in cyclamen breeding. We expect this mutant to become not only a commercial cultivar itself, but also a valuable genetic resource for cyclamen breeding.

Oral presentation

Mutation induction in fragrance cyclamen "Uruwashi-no-kaori" by ion beam irradiation

Kondo, Emiko*; Kameari, Naoko*; Nakayama, Masayoshi*; Kurihara, Yasushi*; Akita, Yusuke; Tanikawa, Natsu*; Morita, Yasumasa*; Hase, Yoshihiro; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

Oral presentation

Development of eficient method for induction of flower color mutants

Okamura, Masachika*; Hase, Yoshihiro; Umemoto, Naoyuki*; Nakayama, Masayoshi*; Tanikawa, Natsu*; Narumi, Issei; Tanaka, Atsushi

no journal, , 

no abstracts in English

Oral presentation

Analyses of flower pigments and volatile compounds of red-purple mutant generated by ion beam irradiation from haploid of fragrant purple cyclamen "Kaori-no-mai"(${it Cyclamen persicum}$ $$times$$ ${it C. purpurascens}$)

Kameari, Naoko*; Nakayama, Masayoshi*; Kondo, Emiko*; Kurihara, Yasushi*; Akita, Yusuke; Hase, Yoshihiro; Tanikawa, Natsu*; Morita, Yasumasa*; Tanaka, Atsushi; Ishizaka, Hiroshi*

no journal, , 

no abstracts in English

33 (Records 1-20 displayed on this page)