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Journal Articles

Analysis of bystander effect induced by cell membrane response in glioma cells

Wada, Seiichi*; Ando, Tatsuhiko*; Watanabe, Aya*; Kakizaki, Takehiko*; Natsuhori, Masahiro*; Funayama, Tomoo; Sakashita, Tetsuya; Yokota, Yuichiro; Kobayashi, Yasuhiko

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 79, 2015/03

So far, we clarified that X-ray irradiation induced cell killing by bystander effect mediated-secreted factor. This phenomenon was related with sphingomyelinase (SMase). In this study we analyzed mechanism of secreted SMase from irradiated cells after irradiation. SMase was detected in the culture medium after irradiation by SDS-PAGE. Then, SMase was detected in the exosome of culture medium, but not out of exosome after irradiation. This result indicates that SMase was secreted as exosome from the irradiated cells.

Journal Articles

Vulnerability of feline T-lymphocytes to charged particles

Kakizaki, Takehiko; Hamada, Nobuyuki*; Sakashita, Tetsuya; Wada, Seiichi*; Hara, Takamitsu*; Funayama, Tomoo; Hodatsu, Tsutomu*; Natsuhori, Masahiro*; Sano, Tadashi*; Kobayashi, Yasuhiko; et al.

Journal of Veterinary Medical Science, 69(6), p.605 - 609, 2007/06

 Times Cited Count:1 Percentile:84.43(Veterinary Sciences)

no abstracts in English

Journal Articles

The Characteristics of radiation-induced cell responses in the canine spontaneous tumor cells

Sano, Tadashi*; Wada, Seiichi*; Suzuki, Keiko*; Natsuhori, Masahiro*; Kakizaki, Takehiko; Ito, Takashi*; Nakazawa, Konomi*; Kobayashi, Yasuhiko; Ito, Nobuhiko*

JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 115, 2007/02

Journal Articles

Killing of feline T-lymphocytes by $$gamma$$-rays and energetic carbon ions

Kakizaki, Takehiko; Hamada, Nobuyuki*; Funayama, Tomoo; Sakashita, Tetsuya; Wada, Seiichi*; Hodatsu, Tsutomu*; Natsuhori, Masahiro*; Sano, Tadashi*; Kobayashi, Yasuhiko; Ito, Nobuhiko*

Journal of Veterinary Medical Science, 68(12), p.1269 - 1273, 2006/12

 Times Cited Count:3 Percentile:74.1(Veterinary Sciences)

no abstracts in English

Journal Articles

Distinct modes of cell death by ionizing radiation observed in two lines of feline T-lymphocytes

Kakizaki, Takehiko; Hamada, Nobuyuki*; Wada, Seiichi*; Funayama, Tomoo; Sakashita, Tetsuya; Hodatsu, Tsutomu*; Sano, Tadashi*; Natsuhori, Masahiro*; Kobayashi, Yasuhiko; Ito, Nobuhiko*

Journal of Radiation Research, 47(3-4), p.237 - 243, 2006/11

no abstracts in English

Journal Articles

Detection of DNA damage induced by heavy ion irradiation in the individual cells with comet assay

Wada, Seiichi; Natsuhori, Masahiro*; Ito, Nobuhiko*; Funayama, Tomoo; Kobayashi, Yasuhiko

Nuclear Instruments and Methods in Physics Research B, 206, p.553 - 556, 2003/05

 Times Cited Count:3 Percentile:71.11

Determining the biological effects of a very low number of charged particles crossing the cell nucleus is interest for estimating the risk due to environmental exposure to charged particles. Especially it is necessary to detect the radiation damage induced by a precise number of charged particles in the individual cells. To compare the number of ions traversing the cell and the DNA damage produced by the hit ions, we applied comet assay. Cells attached on the ion track detector CR-39 were irradiated with 17.3 MeV/u 12C, 15.7 MeV/u, 10.4 MeV/u 20Ne and 6.9 MeV/u 40Ar ion beams at TIARA, JAERI-Takasaki. After irradiation, CR-39 was covered with 1 % agarose. After electrophoresis the CR-39 was taken off from the slide glass. The agarose gel on the CR-39 was stained with ethidium bromide and the opposite side of the CR-39 was etched with KOH-ethanol solution at 37 $$^{circ}C$$. We observed that the ion particles with higher LET value induced the heavier DNA damage, even by the same number of ion-hits within the irradiated cells.

Journal Articles

Fundamental study on radiotherapy of tumors to beneficial companion animals using heavy ion beam

Wada, Seiichi*; Natsuhori, Masahiro*; Kobayashi, Yasuhiko; Yamamoto, Kazuo; Ito, Nobuhiko*

JAERI-Review 2000-024, TIARA Annual Report 1999, p.80 - 82, 2000/10

no abstracts in English

Oral presentation

Modes of cell death of feline T lymphocytes by ionizing radiation

Funayama, Tomoo; Kakizaki, Takehiko; Wada, Seiichi; Hamada, Nobuyuki*; Sakashita, Tetsuya; Hodatsu, Tsutomu*; Yamada, Naoaki*; Sano, Tadashi*; Natsuhori, Masahiro*; Kobayashi, Yasuhiko; et al.

no journal, , 

Responses of two lines of feline T lymphocytes, FeT-J and FL-4, to ionizing radiation were examined in the present study. FL-4 cells, but not FeT-J, were persistently infected with feline immunodeficiency virus. Cells were irradiated with $$^{60}$$Co-$$gamma$$ rays at 2 Gy/min. Surviving fractions were evaluated with clonogenic assay. Apoptosis was detected using TdT-mediated dUTP-biotin nick end labeling (TUNEL) method. Changes in nuclear morphology of viable cells were evaluated by double staining with Hoechst 33342 and ethidium bromide. Mean surviving fractions of FL-4 cells were slightly higher than FeT-J for each radiation dose given. However, dose required to reduce to 63 % of surviving fraction was 1.9 Gy in both cell lines. TUNEL assay, however, revealed the maximum frequency of apoptosis in FL-4 cells ($$<$$20 %) was lower than in FeT-J cells ($$>$$40 %). The time of frequency peak of TUNEL-positive cells in FL-4 was shorter than that of FeT-J. FL-4 cells reached peak level within 24 hours after irradiation, but FeT-J cells needed later than 48 hours after irradiation. Exposure of FL-4 cells to $$gamma$$ rays resulted in the giant- and the multi- nucleus formation. Nuclear swelling occurred to less extent in FeT-J than FL-4 cells. In conclusion, we found the difference in cellular responses to radiation in two lines of feline T lymphocytes. Modes of cell death of FL-4 cells were non-apoptotic and more research would discover new mechanism associated with apoptosis.

Oral presentation

Practical research using the spontaneous neoplasm case of the dog as a particle irradiation treatment model

Sano, Tadashi*; Suzuki, Keiko*; Natsuhori, Masahiro*; Kakizaki, Takehiko; Ito, Nao*; Nakazawa, Konomi*; Ito, Nobuhiko*; Wada, Seiichi*; Kobayashi, Yasuhiko

no journal, , 

no abstracts in English

Oral presentation

Cell killing effects of charged particles on feline T-lymphocytes

Kakizaki, Takehiko; Hamada, Nobuyuki*; Wada, Seiichi*; Sakashita, Tetsuya; Hara, Takamitsu*; Hodatsu, Tsutomu*; Natsuhori, Masahiro*; Sano, Tadashi*; Funayama, Tomoo; Fukamoto, Kana; et al.

no journal, , 

no abstracts in English

Oral presentation

Cell killing effects of charged particles on Melanoma of canine spontaneous tumor

Ito, Takashi*; Wada, Seiichi*; Kakizaki, Takehiko; Kobayashi, Yasuhiko; Natsuhori, Masahiro*; Sano, Tadashi*; Sasaki, Nobuo*; Ito, Nobuhiko*

no journal, , 

no abstracts in English

Oral presentation

Cell killing effects of charged particles on feline T-lymphocytes

Kakizaki, Takehiko; Hamada, Nobuyuki*; Wada, Seiichi*; Sakashita, Tetsuya; Funayama, Tomoo; Hodatsu, Tsutomu*; Natsuhori, Masahiro*; Sano, Tadashi*; Kobayashi, Yasuhiko; Ito, Nobuhiko*

no journal, , 

no abstracts in English

Oral presentation

Cell killing effects of charged particles on Melanoma of canine spontaneous tumor

Ito, Takashi*; Wada, Seiichi*; Kakizaki, Takehiko; Kobayashi, Yasuhiko; Natsuhori, Masahiro*; Sano, Tadashi*; Sasaki, Nobuo*; Ito, Nobuhiko*

no journal, , 

no abstracts in English

13 (Records 1-13 displayed on this page)
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