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Journal Articles

Increase in cell motility by carbon ion irradiation via the Rho signaling pathway and its inhibition by the ROCK inhibitor Y-27632 in lung adenocarcinoma A549 cells

Murata, Kazutoshi*; Noda, Shinei*; Oike, Takahiro*; Takahashi, Akihisa*; Yoshida, Yukari*; Suzuki, Yoshiyuki*; Ono, Tatsuya*; Funayama, Tomoo; Kobayashi, Yasuhiko; Takahashi, Takeo*; et al.

Journal of Radiation Research, 55(4), p.658 - 664, 2014/07

 Times Cited Count:15 Percentile:60.96(Biology)

The effect of carbon ion irradiation on cell motility through the Rho signaling pathway in the human lung adenocarcinoma cell line A549 was studied. At 48 h after irradiation, the cell motility of A549 cells became significantly greater, and the formation of protrusions significantly increased in cells irradiated with carbon ion. The observed increase in cell motility due to carbon ion irradiation was similar to that observed due to X-ray irradiation. Western-blot analysis showed that carbon ion irradiation increased P-MLC2-S19 expression compared with in unirradiated controls, while total MLC2 expression was unchanged. Exposure to a non-toxic concentration of Y-27632, a specific inhibitor of ROCK, reduced the expression of P-MLC2-S19 after C-ion irradiation, resulting in a significant reduction in migration. These data suggest that carbon irradiation increases cell motility in A549 cells via the Rho signaling pathway and that ROCK inhibition reduces that effect.

Journal Articles

Effectiveness of carbon-ion beams for apoptosis induction in rat primary immature hippocampal neurons

Kaminuma, Takuya*; Suzuki, Yoshiyuki*; Shirai, Katsuyuki*; Mizui, Toshiyuki*; Noda, Shinei*; Yoshida, Yukari*; Funayama, Tomoo; Takahashi, Takeo*; Kobayashi, Yasuhiko; Shirao, Tomoaki*; et al.

Journal of Radiation Research, 51(6), p.627 - 631, 2010/11

 Times Cited Count:9 Percentile:37.34(Biology)

Journal Articles

Research into the radiosensitivity of normal brain tissue

Suzuki, Yoshiyuki*; Yoshida, Yukari*; Shirai, Katsuyuki*; Hamada, Nobuyuki*; Mizui, Toshiyuki*; Noda, Shinei*; Funayama, Tomoo; Sakashita, Tetsuya; Kobayashi, Yasuhiko; Shirao, Tomoaki*; et al.

JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 107, 2007/02

Oral presentation

Comparison of biological effects induced by X-rays and heavy ions in the organotypic slice cultures of the rat cerebellum

Yoshida, Yukari*; Suzuki, Yoshiyuki*; Hamada, Nobuyuki*; Shirai, Katsuyuki*; Noda, Shinei*; Al-Jahdari, W. S.*; Funayama, Tomoo; Sakashita, Tetsuya; Wada, Seiichi*; Kobayashi, Yasuhiko; et al.

no journal, , 

no abstracts in English

Oral presentation

Effects of X-ray and carbon ion beam on the postnatal cerebellum in organotypic slice cultures

Suzuki, Yoshiyuki*; Shirai, Katsuyuki*; Yoshida, Yukari*; Mizui, Toshiyuki*; Kiyohara, Hiroki*; Noda, Shinei*; Ishizaki, Yasuki*; Ozawa, Seiji*; Nakano, Takashi*; Hamada, Nobuyuki*; et al.

no journal, , 

no abstracts in English

Oral presentation

Apoptosis induced by heavy charged particles in cultured premature rat neurons

Suzuki, Yoshiyuki*; Shirai, Katsuyuki*; Mizui, Toshiyuki*; Hamada, Nobuyuki*; Noda, Shinei*; Funayama, Tomoo; Yoshida, Yukari*; Kobayashi, Yasuhiko; Shirao, Tomoaki*; Nakano, Takashi*

no journal, , 

no abstracts in English

Oral presentation

Relative biological effectiveness value of carbon ion beam to X-ray on immature hippocampal neurons in vitro

Suzuki, Yoshiyuki*; Shirai, Katsuyuki*; Mizui, Toshiyuki*; Hamada, Nobuyuki*; Noda, Shinei*; Funayama, Tomoo; Yoshida, Yukari*; Kobayashi, Yasuhiko; Shirao, Tomoaki*; Nakano, Takashi*

no journal, , 

no abstracts in English

Oral presentation

Growth cone collapse assay approach to examine sensitivity of cell neurons to carbon beams

Al-Jahdari, W. S.*; Suzuki, Yoshiyuki*; Yoshida, Yukari*; Hamada, Nobuyuki*; Noda, Shinei*; Shirai, Katsuyuki*; Funayama, Tomoo; Sakashita, Tetsuya; Sakurai, Hideyuki*; Kobayashi, Yasuhiko; et al.

no journal, , 

Oral presentation

Biological effectiveness of carbon ions for apoptosis induction in cultured rat premature neurons

Shirai, Katsuyuki*; Suzuki, Yoshiyuki*; Mizui, Toshiyuki*; Hamada, Nobuyuki*; Noda, Shinei*; Funayama, Tomoo; Yoshida, Yukari*; Kobayashi, Yasuhiko; Shirao, Tomoaki*; Nakano, Takashi*

no journal, , 

no abstracts in English

Oral presentation

Neuronal sensitivity to carbon beams; Approach using growth cone collapse assay

Al-Jahdari, W. S.*; Suzuki, Yoshiyuki*; Yoshida, Yukari*; Hamada, Nobuyuki*; Noda, Shinei*; Shirai, Katsuyuki*; Funayama, Tomoo; Sakashita, Tetsuya; Kobayashi, Yasuhiko; Nakano, Takashi*

no journal, , 

Oral presentation

RBE of carbon beams on neurons; Approach using growth cone collapse assay

Al-Jahdari, W. S.*; Suzuki, Yoshiyuki*; Yoshida, Yukari*; Hamada, Nobuyuki*; Okamoto, Masahiko*; Noda, Shinei*; Funayama, Tomoo; Sakashita, Tetsuya; Kobayashi, Yasuhiko; Nakano, Takashi*

no journal, , 

Oral presentation

Increase in cell motility by carbon ion irradiation via the Rho signaling pathway and its inhibition by the ROCK inhibitor Y-27632

Murata, Kazutoshi*; Noda, Shinei*; Oike, Takahiro*; Takahashi, Akihisa*; Yoshida, Yukari*; Suzuki, Yoshiyuki*; Ono, Tatsuya*; Funayama, Tomoo; Kobayashi, Yasuhiko; Takahashi, Takeo*; et al.

no journal, , 

This study aimed to investigate the effect of carbon ion (C-ion) irradiation on cell motility through the ras homolog gene family member (Rho) signaling pathway in the human lung adenocarcinoma cell line A549. Cell motility was assessed by a wound-healing assay, and the formation of cell protrusions was evaluated by F-actin staining. Cell viability was examined by the WST-1 assay. The expression of myosin light chain 2 (MLC2) and the phosphorylation of MLC2 at Ser19 (P MLC2-S19) were analyzed by Western blot. The data suggest that C-ion irradiation increases cell motility in A549 cells via the Rho signaling pathway and that ROCK inhibition reduces that effect.

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