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Journal Articles

Application of laser plasma X-ray beam in radiation biology

Nishikino, Masaharu; Sato, Katsutoshi; Hasegawa, Noboru; Ishino, Masahiko; Kawachi, Tetsuya; Imazono, Takashi; Numasaki, Hodaka*; Teshima, Teruki*; Nishimura, Hiroaki*

X-Ray Lasers 2010; Springer Proceedings in Physics, Vol.136, p.301 - 306, 2011/12

Temporal sequences of physical and physicochemical stage of actions in biological systems irradiated with energetic radiation take place with in the order of picoseconds. The short duration of laser produced plasma source could be used as a new source in contrast to conventional X-ray sources in investigating the mechanism of the affect of radiation on biological cells. We have started to develop a focused X-ray beam irradiation system for use in studying radiobiological effects on cells, and demonstrated a preliminary study of radiation effect on culture cells irradiated with laser produced plasma Ka X-rays and the X-ray laser.

Journal Articles

Development of laser plasma X-ray microbeam irradiation system and radiation biological application

Sato, Katsutoshi; Nishikino, Masaharu; Numasaki, Hodaka*; Kawachi, Tetsuya; Teshima, Teruki*; Nishimura, Hiroaki*

Denki Gakkai Rombunshi, C, 130(10), p.1800 - 1805, 2010/10

no abstracts in English

Journal Articles

$${gamma}$$-H2AX and phosphorylated ATM focus formation in cancer cells after laser plasma X irradiation

Sato, Katsutoshi; Nishikino, Masaharu; Okano, Yasuaki*; Oshima, Shinsuke*; Hasegawa, Noboru; Ishino, Masahiko; Kawachi, Tetsuya; Numasaki, Hodaka*; Teshima, Teruki*; Nishimura, Hiroaki*

Radiation Research, 174(4), p.436 - 445, 2010/10

 Times Cited Count:16 Percentile:53.54(Biology)

Usefulness of laser-plasma X-ray pulse for medical and radiation biological studies was investigated and compared with that of conventional sources such as those obtained from a linear accelerator. A cell irradiation system was developed which uses Copper-K$${alpha}$$ (8 keV) lines from an ultra-short high intensity laser to produce plasma. The absorbed dose of the 8 keV laser-plasma X-ray pulse was accurately estimated with Gafchromic EBT film. When the cells were irradiated with approximately 2 Gy of laser plasma X-ray, the circular regions on $${gamma}$$-H2AX positive cells could be clearly identified. Moreover, the number of $${gamma}$$-H2AX and phosphorylated ataxia telangiectasia mutated (ATM) foci induced by 8 keV laser plasma X-ray were comparable to those induced by 4MV X-ray. These results indicate that the laser plasma X-ray source can be useful for radiation biological studies.

Journal Articles

Application of laser produced plasma K$$alpha$$ X-ray probe in radiation biology

Nishikino, Masaharu; Sato, Katsutoshi; Hasegawa, Noboru; Ishino, Masahiko; Oshima, Shinsuke*; Okano, Yasuaki*; Kawachi, Tetsuya; Numasaki, Hodaka*; Teshima, Teruki*; Nishimura, Hiroaki*

Review of Scientific Instruments, 81(2), p.026107_1 - 026107_3, 2010/02

 Times Cited Count:22 Percentile:70.29(Instruments & Instrumentation)

An X-ray generation and exposure system dedicated for radiation biology is developed. An X-ray pulse of 8.0 keV in energy as short as 1 ps is generated with a fs-laser pulse, and sample cells are irradiated through a specially designed cell dish with a silicon nitride membrane. DNA double-strand breaks in a nucleus of a human caner cell are caused by X-ray irradiation, and similar DNA breaks are obtained to those with the conventional X-ray source. This result demonstrates feasibility of radiobiological study with a single burst of X-rays shining on a single cell specimen.

Journal Articles

Application of laser-accelerated protons to the demonstration of DNA double-strand breaks in human cancer cells

Yogo, Akifumi; Sato, Katsutoshi; Nishikino, Masaharu; Mori, Michiaki; Teshima, Teruki*; Numasaki, Hodaka*; Murakami, Masao*; Demizu, Yusuke*; Akagi, Takashi*; Nagayama, Shinichi*; et al.

Applied Physics Letters, 94(18), p.181502_1 - 181502_3, 2009/05

 Times Cited Count:95 Percentile:94.5(Physics, Applied)

Oral presentation

Development of focused laser plasma X-ray beam for radiobiological applications

Nishikino, Masaharu; Okano, Yasuaki*; Sato, Katsutoshi*; Hasegawa, Noboru; Ishino, Masahiko; Oshima, Shinsuke*; Kawachi, Tetsuya; Numasaki, Hodaka*; Teshima, Teruki*; Nishimura, Hiroaki*

no journal, , 

Recently, high energy, monochromatic X-rays emanating from laser-produced plasma (LPP) are attracting much attention as a new radiation source indispensable for high energy density physics, bioscience, and material sciences. Aiming at these applications, we have been improving performances of Ka lines emitted from and soft X-ray lasers. The monochromaticity is particularly important when X-rays are handled with a narrow-band optics or used to selectively excite a specific material involved in matters. In addition, time-duration of such sources is typically an order of pico-second, and it could be comparable or even shorter than recovery time of radiatively-damaged biological cell, so that the LPP source can be a new source for investigating the mechanisms of irradiation survival and death of biological cells. We propose to develop an ultrashort, intense X-ray microbeam system to study the radiobiological effect and the bystander effect.

Oral presentation

Development of focused laser plasma X-ray beam for radiobiological applications

Nishikino, Masaharu; Ishino, Masahiko; Hasegawa, Noboru; Kawachi, Tetsuya; Sato, Katsutoshi*; Numasaki, Hodaka*; Teshima, Teruki*; Oshima, Shinsuke*; Okano, Yasuaki*; Nishimura, Hiroaki*

no journal, , 

Recently, high energy, monochromatic X-rays emanating from laser-produced plasma (LPP) are attracting much attention as a new radiation source indispensable for high energy density physics, bioscience, and material sciences. Aiming at these applications, we have been improving performances of Ka lines emitted from LPP and soft X-ray lasers (XRLs). The monochromaticity is particularly important when X-rays are handled with a narrowband optics or used to selectively excite a specific material involved in matters. In addition, timeduration of such sources is typically an order of picosecond, and it could be comparable or even shorter than recovery time of radiatively-damaged biological cell, so that the LPP source can be a new source for investigating the mechanisms of irradiation survival and death of biological cells. We propose to develop an ultrashort, intense X-ray microbeam system to study the radiobiological effect and the bystander effect.

Oral presentation

Laser-driven proton beam irradiation on in-vitro human cancer cells

Yogo, Akifumi; Sato, Katsutoshi*; Nishikino, Masaharu; Mori, Michiaki; Teshima, Teruki*; Numasaki, Hodaka*; Murakami, Masao*; Ogura, Koichi; Sagisaka, Akito; Orimo, Satoshi; et al.

no journal, , 

In this work, we demonstrate the irradiation effect of laser accelerated protons induced on human lung cancer cells. In-vitro A549 cells are irradiated with a proton dose of 20 Gy, resulting in a distinct formation of $$gamma$$-H2AX foci as an indicator of DNA double-strand breaks. This is the first result showing that laser-driven ion beams can generate a lethal effect on cancer cells.

Oral presentation

Development of a laser-driven proton irradiation system for a biological study

Yogo, Akifumi; Sato, Katsutoshi*; Nishikino, Masaharu; Mori, Michiaki; Teshima, Teruki*; Numasaki, Hodaka*; Murakami, Masao*; Ogura, Koichi; Sagisaka, Akito; Orimo, Satoshi; et al.

no journal, , 

We have newly developed a proton-beam irradiation system driven by a high-intensity laser for biological studies. In the laser-ion acceleration, X-rays and electrons are generated simultaneously with the protons. In order to remove them, we equiped a magnetic analyzer in the irradiation system. Moreover, we developed a cell capsule, which was newly designed to insert in-vitro cell samples into the vacuum chamber used for the proton generation. We also report the result of 20-Gy proton irradiation of a human lung cancer cells.

Oral presentation

Development of focused laser plasma X-ray beam for radiobiological applications

Nishikino, Masaharu; Sato, Katsutoshi*; Oshima, Shinsuke*; Hasegawa, Noboru; Ishino, Masahiko; Kawachi, Tetsuya; Okano, Yasuaki*; Numasaki, Hodaka*; Teshima, Teruki*; Nishimura, Hiroaki*

no journal, , 

We have started to develop a laser plasma X-ray microbeam irradiation system, and demonstrated a preliminary study of the cell survival and $$gamma$$-H2AX focus formation in the culture cells irradiated with laser-produced plasma X-ray.

Oral presentation

Development of microbeam system using laser-plasma X-ray and identification of DNA double strand breaks in the cancer cell

Sato, Katsutoshi*; Nishikino, Masaharu; Okano, Yasuaki*; Hasegawa, Noboru; Ishino, Masahiko; Oshima, Shinsuke*; Numasaki, Hodaka*; Kawachi, Tetsuya; Teshima, Teruki*; Nishimura, Hiroaki*

no journal, , 

no abstracts in English

Oral presentation

Induction of DNA double strand breaks by laser-plasma quantum beam

Sato, Katsutoshi; Numasaki, Hodaka*; Nishikino, Masaharu; Yogo, Akifumi; Kawachi, Tetsuya; Teshima, Teruki*; Daido, Hiroyuki

no journal, , 

no abstracts in English

Oral presentation

Development of focused laser plasma X-ray beam for radiobiological application

Nishikino, Masaharu; Sato, Katsutoshi; Ishino, Masahiko; Hasegawa, Noboru; Kawachi, Tetsuya; Numasaki, Hodaka*; Teshima, Teruki*; Oshima, Shinsuke*; Nishimura, Hiroaki*

no journal, , 

no abstracts in English

Oral presentation

Development of laser plasma X-ray beam for radiation biology

Nishikino, Masaharu; Sato, Katsutoshi; Ishino, Masahiko; Hasegawa, Noboru; Kawachi, Tetsuya; Numasaki, Hodaka*; Teshima, Teruki*; Oshima, Shinsuke*; Nishimura, Hiroaki*

no journal, , 

no abstracts in English

Oral presentation

Development of focused laser plasma X-ray beam for radiation biology

Nishikino, Masaharu; Sato, Katsutoshi; Ishino, Masahiko; Hasegawa, Noboru; Kawachi, Tetsuya; Numasaki, Hodaka*; Teshima, Teruki*; Oshima, Shinsuke*; Nishimura, Hiroaki*

no journal, , 

no abstracts in English

Oral presentation

Application of laser produced plasma X-ray probe in radiation biology

Nishikino, Masaharu; Sato, Katsutoshi; Ishino, Masahiko; Hasegawa, Noboru; Kado, Masataka; Kawachi, Tetsuya; Numasaki, Hodaka*; Teshima, Teruki*; Okano, Yasuaki*; Oshima, Shinsuke*; et al.

no journal, , 

A dedicated radiation biologyn X-ray generation and exposure system has been dedicated for radiation biology is developed. An X-ray pulse of 8.0 keV in energy X-ray pulses is generated with an fs-laser pulse were used to irradiate, and sample cells are irradiated through a custom-made culture dish with a silicon nitride membrane. The X-ray irradiation resulted in DNA double-strand breaks in thea nucleus of a culture cell are caused by X-ray irradiation that were, and similar DNA breaks are obtained to those obtained with athe conventional X-ray source, thus. This result demonstrating thees feasibility of radiobiological studiesy utilizing with a single burst of X-rays focused shining on a single cell specimens.

Oral presentation

Development of laser plasma X-ray micro beam in radiation biology

Nishikino, Masaharu; Sato, Katsutoshi; Hasegawa, Noboru; Ishino, Masahiko; Kawachi, Tetsuya; Numasaki, Hodaka*; Teshima, Teruki*; Nishimura, Hiroaki*

no journal, , 

Techniques for generating high energy bright short-pulse Ka X-rays have been rapidly developed in recent progress made in utilizing intense ultrashort laser pulses. The soft X-ray laser (XRL) is also generated from the Laser-Produced Plasma (LPP). A highly spatial coherent XRL is generated by the oscillator-amplifier configuration with two targets. Use of X-ray focusing optics has resulted in focused X-ray beams of submicron spot size being achieved in soft and hard X-ray regions. The short duration of laser produced plasma source could be used as a new source in contrast to conventional X-ray sources in investigating the mechanism of the affect of radiation on biological cells.

Oral presentation

Direct observation of P53 mediated bystander effect

Sato, Katsutoshi; Nishikino, Masaharu; Numasaki, Hodaka*; Kawachi, Tetsuya; Teshima, Teruki*; Nishimura, Hiroaki*; Matsuura, Nariaki*

no journal, , 

Recently, the bystander effect induced by ionizing radiation has been reported by many researchers. Many signal transducers such as reactive oxygen species, nitric oxide, molecules involved in mitochondrial functions, and inflammatory cytokines were associated with the bystander effect. Whole phenomenon of the bystander effect must be clarified to understand the apparatus of the radiation effects on the cells. The laser plasma X-ray source has suitable features for this study. To elucidate the bystander effects, we have developed the microbeam system using a laser plasma X-ray, and the feasibilities in the radiation biology have been evaluated. In this research, we report that p53 function is associated with the bystander effect.

Oral presentation

Application of laser plasma X-ray source on radiation biological investigation

Sato, Katsutoshi; Nishikino, Masaharu; Numasaki, Hodaka*; Kawachi, Tetsuya; Teshima, Teruki*; Nishimura, Hiroaki*

no journal, , 

no abstracts in English

Oral presentation

Laser plasma X-ray beam system in JAEA-Kansai Photon Science Institute

Nishikino, Masaharu; Sato, Katsutoshi*; Kawachi, Tetsuya; Nishimura, Hiroaki*; Numasaki, Hodaka*; Teshima, Teruki*

no journal, , 

Techniques for generating high energy bright short-pulse Ka X-rays have been rapidly developed in recent progress made in utilizing intense ultrashort laser pulses. The soft X-ray laser is also generated from the laser-produced plasma. A highly spatial coherent XRL is generated by the oscillator-amplifier configuration with two targets. Use of X-ray focusing optics has resulted in focused X-ray beams of submicron spot size being achieved in soft and hard X-ray regions. The short duration of laser produced plasma source could be used as a new source in contrast to conventional X-ray sources in investigating the mechanism of the affect of radiation on biological cells. We have started to develop a focused X-ray beam irradiation system for use in studying radiobiological effects on cells, and demonstrated a preliminary study of radiation effect on culture cells irradiated with the X-ray laser and laser produced plasma Ka X-rays.

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