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Mori, Kazuhiro*; Okumura, Ryo*; Yoshino, Hirofumi*; Kanayama, Masaya*; Sato, Setsuo*; Oba, Yojiro; Iwase, Kenji*; Hiraka, Haruhiro*; Hino, Masahiro*; Sano, Tadafumi*; et al.
JPS Conference Proceedings (Internet), 33, p.011093_1 - 011093_6, 2021/03
no abstracts in English
Sato, Katsuya; Kikuchi, Masahiro; Ishaque, A. M.*; Oba, Hirofumi*; Yamada, Mitsugu; Tejima, Kohei; Onodera, Takefumi; Narumi, Issei
DNA Repair, 11(4), p.410 - 418, 2012/04
Times Cited Count:25 Percentile:59.62(Genetics & Heredity)In an effort to gain insights into the role of RecFOR proteins in homologous recombination, we generated
,
and
disruptant strains and characterized the disruption effects. Disruption of
resulted in severe reduction of the transformation efficiency. On the other hand, the
disruptant strain was the most sensitive phenotype to
rays, UV irradiation and mitomycin C among the three disruptants. In the
disruptant strain, the intracellular level of the LexA1 protein did not decrease following
irradiation. These results demonstrate that the RecF protein plays a crucial role in the homologous recombination repair process by facilitating RecA activation. Thus, the RecF and RecR proteins are involved in the RecA activation and the stability of incoming DNA, respectively, during RecA-mediated homologous recombination processes that initiated the ESDSA pathway in
.
Sghaier, H.*; Sato, Katsuya; Oba, Hirofumi*; Narumi, Issei
African Journal of Biochemistry Research, 4(4), p.111 - 118, 2010/04
Yang, Y.*; Ito, Takashi*; Yokobori, Shinichi*; Shimada, Haruo*; Itahashi, Shiho*; Sato, Katsuya; Oba, Hirofumi*; Narumi, Issei; Yamagishi, Akihiko*
International Journal of Systematic and Evolutionary Microbiology, 60, p.776 - 779, 2010/04
Times Cited Count:27 Percentile:50.65(Microbiology)Sato, Katsuya; Tu, Z.*; Oba, Hirofumi*; Narumi, Issei
JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 81, 2009/12
no abstracts in English
Sato, Katsuya; Tu, Z.*; Oba, Hirofumi; Narumi, Issei
Plasmid, 62(1), p.1 - 9, 2009/07
Times Cited Count:11 Percentile:27.88(Genetics & Heredity)To develop new shuttle vectors for species, the nucleotide sequence of the small cryptic plasmid pUE30 from Deinococcus radiopugnans ATCC19172 was determined. The 2,467-bp plasmid possesses two open reading frames, one encoding 88 amino acid residues (Orf1) and the other encoding 501 amino acid residues (Orf2). The predicted amino acid sequence encoded by Orf1 exhibits similarity to the N-terminal regions of replication proteins encoded by
-type plasmids of
-proteobacteria. On the other hand, the predicted amino acid sequence encoded by Orf2 exhibits similarity to replication proteins encoded by plasmids of
SARK and
species. Hybrid plasmids consisting of pUE30 and pKatCAT5, which replicates in
with a chloramphenicol resistance determinant, were shown to autonomously replicate in
ATCC43672. Deletion analysis revealed that Orf2 was necessary for replication of the plasmids in
. On the other hand, a DNA fragment encompassing the Orf1-coding region was involved in the instability of the plasmid in
. An expression plasmid that possesses the
minimal
promoter was constructed, and a firefly luciferase gene was successfully expressed in
. The
host-vector system developed in this study should prove useful in the bioremediation of radioactive waste and for the investigation of DNA repair mechanisms.
Oba, Hirofumi; Sato, Katsuya; Sghaier, H.; Yanagisawa, Tadashi*; Narumi, Issei
Extremophiles, 13(3), p.471 - 479, 2009/05
Times Cited Count:20 Percentile:39.50(Biochemistry & Molecular Biology) possesses a DNA damage response mechanism that acts via the PprI protein to induce RecA and PprA proteins, both of which are necessary in conferring extreme radioresistance. In an effort to further delineate the nature of the DNA damage response mechanism in
, we set out to identify novel components of the PprI-dependent signal transduction pathway in response to radiation stress. Here we demonstrate the discovery of a novel regulatory protein, PprM (a modulator of the PprI-dependent DNA damage response), which is a homolog of cold shock protein (Csp). Disruption of the
gene rendered
significantly sensitive to
-rays. PprM regulates the induction of PprA but not that of RecA. PprM belongs in a distinct clade of a subfamily together with Csp homologs from
and
. Purified PprM is present as a homodimer under physiological conditions, as the case with
CspD. The
double-disruptant strain exhibited higher sensitivity than the
or
single disruptant strains, suggesting that PprM regulates other hitherto unknown protein(s) important for radioresistance besides PprA. This study strongly suggests that PprM is involved in the radiation response mediated by PprI in
.
Suzuki, Michiyo; Sakashita, Tetsuya; Yanase, Sumino*; Kikuchi, Masahiro; Oba, Hirofumi; Higashitani, Atsushi*; Hamada, Nobuyuki*; Funayama, Tomoo; Fukamoto, Kana; Tsuji, Toshio*; et al.
Journal of Radiation Research, 50(2), p.119 - 125, 2009/04
Times Cited Count:8 Percentile:28.94(Biology)Yang, Y.*; Ito, Takashi*; Yokobori, Shinichi*; Itahashi, Shiho*; Shimada, Haruo*; Sato, Katsuya; Oba, Hirofumi; Narumi, Issei; Yamagishi, Akihiko*
International Journal of Systematic and Evolutionary Microbiology, 59, p.1862 - 1866, 2009/00
Times Cited Count:32 Percentile:55.46(Microbiology)An orange pigmented, non-motile, coccoid bacterial strain, TR0125, was isolated from dust samples collected in the high atmosphere above Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that it was within the radiation of species. Major peptidoglycan amino acids were D-glutamic acid, glycine, D-alanine, L-alanine and ornithine. Predominant fatty acids were 17:0 iso, iso 17:1
9c and 15:0 iso. Strong resistance to desiccation, UV-C and
-radiation, high DNA G+C content also supported the affiliation of strain TR0125
to the genus
. TR0125
had a highest similarity value (95.7 %) of the 16S rRNA gene sequence to the type strain of the species
, and phylogenetic analysis shows that it was further separated from
than from
, indicating that strain TR0125
was not a member of these two
species. Besides, there were phenotypic differences between strain TR0125
and type strains of the two species. Therefore, we propose a new species of the genus
,
sp. nov. (type strain, TR0125
= JCM 11750
= DSM 21212
), to accommodate this isolate.
Oba, Hirofumi; Sato, Katsuya; Kikuchi, Masahiro; Sghaier, H.; Yanagisawa, Tadashi*; Narumi, Issei
JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 57, 2008/11
no abstracts in English
Oba, Hirofumi; Sato, Katsuya; Narumi, Issei
Hoshasen To Sangyo, (118), p.50 - 53, 2008/06
no abstracts in English
Sato, Katsuya; Oba, Hirofumi; Sghaier, H.; Narumi, Issei
JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 92, 2008/03
no abstracts in English
Sghaier, H.; Narumi, Issei; Sato, Katsuya; Oba, Hirofumi; Mitomo, Hiroshi*
Theory in Biosciences, 126(1), p.43 - 45, 2007/03
Times Cited Count:15 Percentile:84.13(Biology)no abstracts in English
Oba, Hirofumi; Sato, Katsuya; Yanagisawa, Tadashi*; Narumi, Issei
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 70, 2007/02
no abstracts in English
Narumi, Issei; Oba, Hirofumi; Sghaier, H.; Sato, Katsuya
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 69, 2007/02
no abstracts in English
Sato, Katsuya; Oba, Hirofumi; Sghaier, H.; Narumi, Issei
Microbiology, 152(11), p.3217 - 3226, 2006/11
In an effort to gain an insight into the role of LexA2 in the radiation response mechanism, disruptant strain was generated and investigated. The
disruptant strains exhibited a much higher resistance to
rays than the wild-type strain. Furthermore, a luciferase assay showed that
promoter activation was enhanced in the
disruptant strain following
irradiation. The increase in radioresistance of the
disruptant strain is explained in part by the enhancement of
promoter activation.
Sato, Katsuya; Oba, Hirofumi; Narumi, Issei
Seibutsu Butsuri, 46(5), p.270 - 274, 2006/09
no abstracts in English
Oba, Hirofumi*; Sato, Katsuya*; Yanagisawa, Tadashi*; Narumi, Issei
Gene, 363, p.133 - 141, 2005/12
Times Cited Count:37 Percentile:56.92(Genetics & Heredity)Three transcriptional start points for the were located at positions -156, -154 and -22 upstream from the
translation initiation site. The amount of the three extended products increased in cells exposed to 2-kGy followed by a 0.5-h post-incubation, suggesting the existence of at least two radiation responsive promoters for
expression. A luciferase reporter assay revealed that the distal promoter is located between positions -208 and -156 from the translation initiation site, while the proximal promoter is located between positions -57 and -22. The region located between positions -57 and -38 was indispensable for proximal promoter activity. Site-directed mutagenesis of a thymine positioned at -33 resulted in severe impairment of promoter activity, and suggested that the thymine functions as a master base for the proximal radiation responsive promoter. The results also suggested that up-regulation of
expression by the
gene product is triggered at the promoter level.
Islam, M. S.*; Hua, Y.*; Oba, Hirofumi; Sato, Katsuya; Kikuchi, Masahiro; Yanagisawa, Tadashi*; Narumi, Issei
Genes and Genetic Systems, 78(5), p.319 - 327, 2003/10
Times Cited Count:16 Percentile:30.71(Biochemistry & Molecular Biology)no abstracts in English
Sato, Katsuya; Tokue, Masaru*; Hara, Masaki*; Katayama, Takeshi*; Oba, Hirofumi; Narumi, Issei
no journal, ,
no abstracts in English