Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Kitabatake, Satomi*; Ushiroda, Tota*; Hirayama, Ryoichi*; Furusawa, Yoshiya*; Funayama, Tomoo; Yokota, Yuichiro; Okahata, Yoshio*; Ito, Atsushi*
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 86, 2015/03
Biological effects of high-LET radiation could be understood in terms of the ion track structure. Therefore the evaluation of the contribution of both core and penumbra regions to biological effects is an important issue for the study of high-LET effects. In the present study, we developed a protocol to make a uniform DNA sheet with insoluble nature in aqueous solution, and explored the applicability to the detection of 8-OHdG distributions after heavy-ion irradiation. Water-insoluble DNA sheet was irradiated with proton and neon ion beams at JAEA-Takasaki. After irradiation DNA samples were incubated with an 8-OHdG antibody followed by with a second antibody containing a fluorescence probe. The preliminary results indicated that upon ion irradiation randomly distributed dot-like fluorescence was observed, suggesting that these dots may be from incident ions.
Hirayama, Ryoichi*; Uzawa, Akiko*; Takase, Nobuhiro*; Matsumoto, Yoshitaka*; Noguchi, Miho; Koda, Kana*; Ozaki, Masakuni*; Yamashita, Kei*; Li, H.*; Kase, Yuki*; et al.
Mutation Research; Genetic Toxicology And Environmental Mutagenesis, 756(1-2), p.146 - 151, 2013/08
Times Cited Count：24 Percentile：62.77(Biotechnology & Applied Microbiology)Hirayama, Ryoichi*; Matsumoto, Yoshitaka*; Noguchi, Miho; Uzawa, Akiko*; Koda, Kana*; Furusawa, Yoshiya*
no journal, ,
The presence or absence of molecular oxygen dramatically influences the biological effect of low LET radiations. To produce oxygen effect, molecular oxygen must be present during the radiation exposure or at least during the lifetime of the free radicals generated by the radiation. Little study has been done to actually investigate the influence of oxygen after the radiation exposure. The present study was undertaken in order to explore the rejoining activity of DNA-DSB induced by anaerobic X-ray or carbon ion (
80 keV/
m) irradiations under oxic and hypoxic holdings (37 
C). DNA-DSB in CHO cells were analyzed by a static-field gel electrophoresis. The kinetics of the rejoining could be described by a sum of fast and slow components. The slow component of DNA-DSB induced by X-ray under oxic incubation was faster than that under hypoxic incubation. Furthermore, the percentages of non-reparable DNA damage were 5% and 20% under oxic and hypoxic incubation conditions, respectively. However, no difference between oxic and hypoxic incubation conditions was found for carbon ion irradiation. There results indicate that molecular oxygen influences the rejoining of DNA-DSB after low LET radiation exposure.
Fukushima, Keitaro*; Iwasaki, Kenta*; Oda, Yoshiya*; Sakai, Masaru*; Katata, Genki*; Yamaguchi, Takashi*; Nakayama, Masataka*; Kubota, Tomohiro*; Nagano, Hirohiko; Watanabe, Makoto*; et al.
no journal, ,
no abstracts in English
Fukushima, Keitaro*; Iwasaki, Kenta*; Oda, Yoshiya*; Sakai, Masaru*; Katata, Genki*; Yamaguchi, Takashi*; Kubota, Tomohiro*; Nagano, Hirohiko; Watanabe, Makoto*; Koarashi, Jun
no journal, ,
Nitrogen retention in the forest ecosystem can be mainly evaluated as a balance between input of atmospheric reactive N and hydrological N loss at a watershed scale. As an assumption for the evaluation, it has been proposed that the watershed is completely "closed", namely, the source of water flowing out from the watershed is rainwater fell into the watershed. This means that exchange of N transported with water between the watershed and the adjacent watershed beyond the watershed boundary is ignored. However, is this assumption applicable for all forested watersheds? If not, nitrogen retention in the watershed may not be evaluated accurately. In our presentation, we aim to elucidate the source of streamwater nitrate-N in the forested watershed whose water balance are not possibly closed.
Fukushima, Keitaro*; Iwasaki, Kenta*; Oda, Yoshiya*; Sakai, Masaru*; Katata, Genki*; Yamaguchi, Takashi*; Kubota, Tomohiro*; Nagano, Hirohiko*; Watanabe, Makoto*; Koarashi, Jun
no journal, ,
no abstracts in English
