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Tanaka, Naritake*; Kimura, Hitoshi*; Faried, A.*; Sakai, Makoto*; Sano, Takaaki*; Inose, Takanori*; Soda, Makoto*; Okada, Koji*; Nakajima, Masanobu*; Miyazaki, Tatsuya*; et al.
Cancer Science, 101(6), p.1487 - 1492, 2010/06
Times Cited Count:12 Percentile:31.68(Oncology)We examined the intracellular localization of cisplatin, a key chemotherapeutic agent, in esophageal cancer cell lines and determined their sensitivity to cisplatin using in-air micro-PIXE. Two human esophageal squamous cell carcinoma (ESCC) cell lines, TE-2 and TE-13, were examined for their response to cisplatin using MTT assay, flow cytometry and DNA fragmentation assays. Real-time reverse transcription-polymerase chain reaction was also used to evaluate the mRNA expression of multidrug resistance protein 2 (MRP2) in both cell lines. Platinum localizations of intracellular and intranuclear were measured using in-air micro-PIXE. TE-2 cells were more sensitive to cisplatin than TE-13 cells. The results of this study suggest that in-air micro-PIXE could be a useful quantitative method for evaluating the cisplatin sensitivity of individual cells. Finally, we speculate that MRP2 in the cell membrane may play an important role in regulating cisplatin sensitivity of ESCC cells.
Kuroha, Mitsuo; Takeda, Kunio; Iitsuka, Shoji; Sasaki, Shuichi; Okada, Toshio; Isozaki, Mikio; Daigo, Yoshimichi; Sato, Minoru
PNC TN941 81-49, 204 Pages, 1981/05
PNC type in-sodium hydrogen meters have been developed as leak detectors for LMFBR MONJU steam generators. In order to confirm the long-term reliability and the durability of the meters, the four meters were installed in three sodium loops at the O-arai Engineering Center, and they had been tested over a long time in flowing sodium. A period of the test was from oct. 1977 to Feb. 1980. They are called type II. The dynamic chamber of the vacuum system can separate from the static one, and be also connected with it using one flexible tube. Important findings from the test are; (1) The operating time of two meters exceeded 10,000 hours, and the total of all meters was about 35,000 hours. No trouble had been experienced in the sodium systems and the nickel membranes of them during the period, which had the good durability. Air leaks, however, occured three times in the two vacuum systems. (2) Any secular changes had hardly happened in the permeability of hydrogen through the nickel membrane and the relationship between ion pump current and hydrogen pressure. (3) The pumping speeds had decreased with increasing the amount of absorbed hydrogen. The decreasing rates differed among four ion pumps, and those of two pumps were considerably large at the beginning of absorbing hydrogen. (4) The calibration curves, which describe the relationship between hydrogen concentration in sodium and ion pump current, had changed with time. The largest cause was the decrement of the pumping speeds. (5) The UHV gauges were superior to the ion pumps from the point of the signal-to-noise ratio as the hydrogen sensor.
Kamemoto, Yuichiro; Shiba, Koreyuki; Handa, Nuneo; Okada, Minoru
JAERI 4019, 20 Pages, 1961/10
no abstracts in English