Effect of minerals on accumulation of Cs by fungus

Onuki, Toshihiko; Sakamoto, Fuminori; Yamasaki, Shinya*; Kozai, Naofumi; Shiotsu, Hiroyuki; Utsunomiya, Satoshi*; Watanabe, Naoko*; Kozaki, Tamotsu*

Journal of Environmental Radioactivity, 144, p.127 - 133, 2015/06

https://doi.org/10.1016/j.jenvrad.2015.02.018

The accumulation of Cs by unicellular fungus of in the presence of minerals has been studied to elucidate the role of microorganisms in the migration of radioactive Cs in the environment. In the presence of minerals in the agar medium, the radioactivity in the yeast cells was in the order of mica smectite, illite vermiculite, phlogopite, zeolite. This order is inversely correlated to the ratio of the concentration of radioactive Cs between the minerals and the medium solution. These results strongly suggest that the yeast accumulates radioactive Cs competitively with minerals.

Identification of DNA mutation sites in a high temperature tolerant mutant of USDA110 generated by ion-beam irradiation

Takeda, Kiyoko; Sato, Katsuya; Narumi, Issey*; Otsu, Naoko*; Yokoyama, Tadashi*

JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 114, 2014/03

Nutritional physiology of essential elements in higher plants

Makino, Amane*; Sueyoshi, Kuni*; Suzuki, Yuji*; Kawachi, Naoki; Tawaraya, Keitaro*; Otsu, Naoko*; Nakanishi, Hiromi*; Fujiwara, Toru*

Nihon Dojo Hiryo Gaku Zasshi, 82(2), p.152 - 159, 2011/04

https://doi.org/10.20710/dojo.82.2_152

This paper is a proceeding of the fourth division (Plant nutrition) symposium theme of discussed in the annual conference of 2011 soil science and plant nutrition at Hokkaido University. N. Kawachi, an author who takes charge of third section, discuss nutritional physiology revealed by imaging analysis of element kinetics in plants. This section focuses on introducing a RI imaging system developed in Japan Atomic Energy Agency. In addition, it is expressed that the analytical research conducted on nutritional physiology in plants as revealed by imaging of element kinetics.

Genome analysis of a high temperature tolerant mutant of USDA110 generated by ion-beam microbial mutation-breeding technology

Takeda, Kiyoko; Sato, Katsuya; Narumi, Issey*; Otsu, Naoko*; Yokoyama, Tadashi*

no journal, , 

We have tried to generate high temperature tolerant mutants of USDA110 using ion beam breeding technology. Consequently, we obtained a mutant, M14, which can survive at 42C for at least 7 days. The genome sequence data of M14 were accumulated using a GS Junior System. The data were then mapped to the reference sequence of strain USDA110 using a GS Reference Mapper software. About 99.5% of the reference sequence was covered with the M14 sequence. Genome comparison revealed a large-scale structural change in the circular M14 genome. Namely, a DNA region at the coordinates 4,029,225 5,299,697 (1,270,473 bp) was inverted. In addition, about 20 mutations such as base substitutions, insertions and deletions were found in M14. The acquirement of high temperature tolerance in M14 could be attributed to a large-scale inversion and/or small-scale mutations.

Identification of DNA mutation sites in a high temperature tolerant mutant of USDA110 generated by ion-beam irradiation

Takeda, Kiyoko; Sato, Katsuya; Narumi, Issey*; Otsu, Naoko*; Yokoyama, Tadashi*

no journal, , 

With the aims of utilization as a biofertilizer inoculant and elucidation of high-temperature tolerance mechanism of soybean nodule bacteria, we had applied the ion beam breeding technology to a strain () USDA110. Consequently, we obtained a mutant named M14 which was able to maintain high viable cell numbers under high-temperature (42C) for at least 7 days. Genome comparison with USDA110 revealed that (1) 1.27 Mbp inversion mutagenesis and (2) 18 single base mutations were occurred in M14. This study was the first attempt to apply the ion beam breeding technology to a prokaryote and succeeded in proving the effectiveness of it.

Genome structure and base mutations in the high temperature tolerant mutant of USDA110 generated by ion-beam irradiation

Takeda, Kiyoko*; Sato, Katsuya; Ono, Yutaka; Narumi, Issey*; Otsu, Naoko*; Yokoyama, Tadashi*

no journal, , 

no abstracts in English