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Journal Articles

Effect of ionizing radiation upon dehydrated Pv11 cultured cells originated from the sleeping chironomid

Watanabe, Kazuyo*; Akitsuki, Takashi*; Shimura, Sachiko*; Gusev, O.*; Cornette, R.*; Kikawada, Takahiro*; Sakashita, Tetsuya; Funayama, Tomoo; Kobayashi, Yasuhiko; Okuda, Takashi*

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 87, 2015/03

The Sleeping Chironomid, ${it Polypedilum vanderplanki}$ can stand complete desiccation (anhydrobiosis) and also shows radio-resistance. Recently, we have generated cultured cell (Pv11) originated from ${it P. vanderplanki}$ embryo which can also stand complete dehydration. In this study, we examine the tolerance of cultured cell Pv11 against ionbeam irradiation.

Journal Articles

Development of the irradiation method for the first instar silkworm larvae using locally targeted heavy-ion microbeam

Fukamoto, Kana; Shirai, Koji*; Sakata, Toshiyuki*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Wada, Seiichi*; Kakizaki, Takehiko; Shimura, Sachiko*; Kobayashi, Yasuhiko; et al.

Journal of Radiation Research, 48(3), p.247 - 253, 2007/05

 Times Cited Count:17 Percentile:47.23(Biology)

To carry out the radio-microsurgery study using silkworm, ${it Bombyx mori}$, we have already developed the specific irradiation systems for eggs and third to fifth instar larvae. In this study, a modified application consisting of the first instar silkworm larvae was further developed using heavy-ion microbeams. This system includes aluminum plates with holes specially designed to fix the first instar silkworm larvae during irradiation, and Mylar films were used to adjust energy deposited for planning radiation doses at certain depth. Using this system, the suppression of abnormal proliferation of epidermal cells in the knob mutant was examined. Following target irradiation of the knob-forming region at the first instar stage with 180-mum-diameter microbeam of 220 MeV carbon ($$^{12}$$C) ions, larvae were reared to evaluate the effects of irradiation. The results indicated that the knob formation at the irradiated segment was specially suppressed in 5.9, 56.4, 66.7 and 73.6 % of larvae irradiated with 120, 250, 400 and 600 Gy, respectively, but the other knob formations at the non-irradiated segments were not suppressed in either irradiation. Although some larva did not survive undesired non-targeted exposure, our present results indicate that this method would be useful to investigate the irradiation effect on a long developmental period of time. Moreover, our system could also be applied to other species by targeting tissues, or organs during development and metamorphosis in insect and animals.

Journal Articles

Effects of heavy-ion irradiation on the differentiation of epidermal cells in the silkworm, ${it Bombyx mori}$

Fukamoto, Kana; Shimura, Sachiko*; Shirai, Koji*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Sakashita, Tetsuya; Funayama, Tomoo; Kobayashi, Yasuhiko

Journal of Insect Biotechnology and Sericology, 75(3), p.107 - 114, 2006/10

no abstracts in English

Oral presentation

R&D of lithium target and test facilities for the fusion neutron source

Wakai, Eiichi; Kondo, Hiroo; Kanemura, Takuji; Hirakawa, Yasushi; Furukawa, Tomohiro; Kikuchi, Takayuki; Ito, Yuzuru*; Hoashi, Eiji*; Yoshihashi, Sachiko*; Horiike, Hiroshi*; et al.

no journal, , 

no abstracts in English

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