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論文

Mutation induction by carbon ion beam irradiation in banana (${it Musa}$ spp.)

Reyes-Borja, W. O.*; Sotomayor, I.*; Garz$'o$n, I.*; Vera, D.*; Cede$~n$o, M.*; 田中 淳; 長谷 純宏; 瀬古澤 由彦*; 菅谷 純子*; 弦間 洋*

JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 89, 2008/03

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2007-060.pdf

Banana (${it Musa acuminata}$ AAA) is a world cultivated crop around tropical and subtropical areas. Black Sigatoka disease caused by ${it Mycosphaerella fijiensis}$ Morelet, is the most destructive disease and inveterate of this crop. Thus, radiation breeding could prove to be a viable method in banana breeding work. Carbon ion beam irradiation was applied to study critical doses, genetic variability and the response to black Sigatoka disease. Eight Gy was supposed to be the best dose to irradiate both banana cultivars. Eight plants were selected as possible candidates with a better response to black Sigatoka. In addition, a fast growing plant was observed and selected as earliness being an important characteristic for shortening the crop life cycle.

論文

Alteration of resistance to black Sigatoka (${it Mycosphaerella fijiensis}$ Morelet) in banana by ${it in vitro}$ irradiation using carbon ion-beam

Reyes-Borja, W. O.*; Sotomayor, I.*; Garz$'o$n, I.*; Vera, D.*; Cede$~n$o, M.*; Castillo, B.*; 田中 淳; 長谷 純宏; 瀬古澤 由彦*; 菅谷 純子*; et al.

Plant Biotechnology, 24(3), p.349 - 353, 2007/06

https://doi.org/10.5511/plantbiotechnology.24.349
 被引用回数：13 パーセンタイル：33.78(Biotechnology & Applied Microbiology)

Carbon-ion beam was applied to explants of banana "Cavendish Enano" and "Williams" cultivars, in order to study the critical doses, genetic variability and response to black Sigatoka disease; doses employed were: 0, 0.5, 1, 2, 4, 8, 16, 32, 64 and 128 Gy. Biological effect of each dose on height (cm), weight (g), survival rate (%) was recorded. For black Sigatoka assessment on regenerated plants, the disease development period (DDP-days), infection index (II-%) and leaf disc necrotic area (LDNA-%) by juglone toxin were measured. As results, the mortality was increased in explants exposed to higher doses. Weight and height were reduced by doses of 16-128 Gy. Six plants of "Williams" (code number: "W16II74", "W128I67", "W1II148", "W8II13", "W1II19" and "W1II31") and two plants of "Cavendish Enano" (code numbers: "CE4II30" and "CE64I5") were selected as candidates by virtue of being less affected by the disease and by the toxin.

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