Autoradiography system with phosphor powder (ZnS:Ag) for imaging radioisotope dynamics in a living plant

Kurita, Keisuke; Sakai, Takuro; Suzui, Nobuo*; Yin, Y.-G.*; Sugita, Ryohei*; Kobayashi, Natsuko*; Tanoi, Keitaro*; Kawachi, Naoki*

Japanese Journal of Applied Physics, 60(11), p.116501_1 - 116501_4, 2021/11

https://doi.org/10.35848/1347-4065/ac2b50

Radioisotope tracer imaging is useful for studying plant physiological phenomena. In this study, we developed an autoradiography system with phosphor powder (ZnS:Ag), "Live-autoradiography", for imaging radioisotope dynamics in a living plant. This system visualizes the element migration and accumulation in intact plants continuously under a light environment. An imaging test was performed on point sources of Cs, with a radioactivity of 10-100 kBq of being observed; this indicates satisfactory system linearity between the image intensity and the radioactivity of Cs. Moreover, dynamics imaging of Cs was performed on an intact soybean plant for four days. The serial images indicated Cs accumulation in the node, vein, and growing point of the plant. The developed system can be used for studying plant physiological phenomena and can be employed for quantitative measurement of radionuclides.

Non-invasive imaging of radiocesium dynamics in a living animal using a positron-emitting Cs tracer

Suzui, Nobuo*; Shibata, Takuya; Yin, Y.-G.*; Funaki, Yoshihito*; Kurita, Keisuke; Hoshina, Hiroyuki*; Yamaguchi, Mitsutaka*; Fujimaki, Shu*; Seko, Noriaki*; Watabe, Hiroshi*; et al.

Scientific Reports (Internet), 10, p.16155_1 - 16155_9, 2020/10

https://doi.org/10.1038/s41598-020-73351-2

Imaging of radiocesium dynamics in soybean by using a high-resolution gamma camera

Yin, Y.-G.; Kawachi, Naoki; Suzui, Nobuo; Ishii, Satomi; Yoshihara, Toshihiro*; Watabe, Hiroshi*; Yamamoto, Seiichi*; Fujimaki, Shu

JAEA-Review 2015-022, JAEA Takasaki Annual Report 2014, P. 112, 2016/02

https://doi.org/10.11484/jaea-review-2015-022

Evaluation of the effect of elevated concentrations of CO in a greenhouse for tomato cultivation

Ishii, Satomi; Yamazaki, Haruaki*; Suzui, Nobuo; Yin, Y.-G.; Kawachi, Naoki; Shimada, Hiroaki*; Fujimaki, Shu

JAEA-Review 2015-022, JAEA Takasaki Annual Report 2014, P. 93, 2016/02

https://doi.org/10.11484/jaea-review-2015-022

Base to tip and long-distance transport of sodium in the root of common reed [ (Cav.) Trin. ex Steud.] at steady state under constant high-salt conditions

Fujimaki, Shu; Maruyama, Teppei*; Suzui, Nobuo; Kawachi, Naoki; Miwa, Eitaro*; Higuchi, Kyoko*

Plant & Cell Physiology, 56(5), p.943 - 950, 2015/05

https://doi.org/10.1093/pcp/pcv021

We analyzed the directions and rates of translocation of sodium ions (Na) within tissues of a salt-tolerant plant, common reed, and a salt-sensitive plant, rice, under constant high-salt conditions using radioactive Na tracer and a positron-emitting tracer imaging system (PETIS). First, the test plants were incubated in a nutrient solution containing 50 mM NaCl and a trace level of Na for 24 h (feeding step). Then the original solution was replaced with a fresh solution containing 50 mM NaCl but no Na, in which the test plants remained for over 48 h (chase step). Non-invasive visualization of Na movement in the test plants was conducted during feeding and chase steps with PETIS. Our results revealed that Na was absorbed in the roots of common reed, but not transported to the upper shoot beyond the shoot base. During the chase step, a basal-to-distal movement of Na was detected within the root tissue with a velocity of approximately 0.5 cm h. On the other hand, Na that absorbed in the roots of rice was continuously translocated to the whole shoot. We concluded that the basal roots and the shoot base of common reed have constitutive functions of Na exclusion only in the direction of root tips, even under constant high-salt conditions. This function may apparently contribute to the low Na concentration in the upper shoot and high salt tolerance of common reed.

Common reed accumulates starch in its stem by metabolic adaptation under Cd stress conditions

Higuchi, Kyoko*; Kanai, Masatake*; Tsuchiya, Masahisa*; Ishii, Haruka*; Shibuya, Naofumi*; Fujita, Naoko*; Nakamura, Yasunori*; Suzui, Nobuo; Fujimaki, Shu; Miwa, Eitaro*

Frontiers in Plant Science (Internet), 6, p.138_1 - 138_6, 2015/03

https://doi.org/10.3389/fpls.2015.00138

Ultra-high resolution of radiocesium distribution detection based on Cherenkov light imaging

Yamamoto, Seiichi*; Ogata, Yoshimune*; Kawachi, Naoki; Suzui, Nobuo; Yin, Y.-G.; Fujimaki, Shu

Nuclear Instruments and Methods in Physics Research A, 777, p.102 - 109, 2015/03

https://doi.org/10.1016/j.nima.2014.12.090

From laboratory to field; -knockdown rice is a promising candidate for Cd phytoremediation in paddy fields

Takahashi, Ryuichi*; Ishimaru, Yasuhiro*; Shimo, H.*; Bashir, K.*; Senoura, Takeshi*; Sugimoto, Kazuhiko*; Ono, Kazuko*; Suzui, Nobuo; Kawachi, Naoki; Ishii, Satomi; et al.

PLOS ONE (Internet), 9(6), p.e98816_1 - e98816_7, 2014/06

https://doi.org/10.1371/journal.pone.0098816

Whole-plant imaging of Cd distribution using positron-emitting tracer imaging system

Suzui, Nobuo; Yin, Y.-G.; Igura, Masato*; Ishii, Satomi; Kawachi, Naoki; Ishikawa, Satoru*; Fujimaki, Shu

JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 101, 2014/03

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2013-059.pdf

Analysis of the effect of O partial pressure on nitrogen fixation in soybean plant using positron-emitting tracer

Hung, N. V. P.; Ishii, Satomi; Suzui, Nobuo; Kawachi, Naoki; Yin, Y.-G.; Koyanagi, Atsushi; Oyama, Takuji*; Fujimaki, Shu

JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 99, 2014/03

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2013-059.pdf

The impacts of oxygen in rhizosphere on the symbiotic nitrogen fixation in real-time was evaluated under various O partial pressure. Soybean nodules were treated with mixed gas containing N labeled with a radioisotope N and various concentrations of O, and the nitrogen fixation activity in the nodules was visualized by the positron-emitting tracer imaging system (PETIS). The results showed that under normal condition the nitrogen fixation ability of soybean plant was higher compared to that of under the other conditions (0% O and 10% O). There was no significant difference in the nitrogen fixation activity between 0% O and 10% O.