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Yamauchi, Emiko*; Watanabe, Ritsuko; Oikawa, Miyoko*; Fujimoto, Hirofumi*; Yamada, Akinori*; Saito, Kimiaki; Murakami, Masahiro*; Hashido, Kazuo*; Tsuchida, Kozo*; Takada, Naoko*; et al.
Journal of Insect Biotechnology and Sericology, 77(1), p.17 - 24, 2008/02
The frequency of single strand breaks (SSBs) occurring on both strands of the pBR322 plasmid DNA region flanked by a pair of primers used for polymerase chain reaction (PCR) amplifications was determined after irradiation with 
Cs 
-rays. We refined that real time PCR is suitable for the detection and quantitative analysis of SSBs caused by -ray irradiation. The utility of this approach was also supported by the comparison of the practical experimental data with the Monte Carlo simulation. The potential application of this PCR method for the detection of genomic DNA damage was also confirmed.
Endo, Satoru*; Tanaka, Kenichi*; Takada, Masashi*; Onizuka, Yoshihiko*; Miyahara, Nobuyuki*; Sato, Tatsuhiko; Ishikawa, Masayoshi*; Maeda, Naoko*; Hayabuchi, Naofumi*; Shizuma, Kiyoshi*; et al.
Medical Physics, 34(9), p.3571 - 3578, 2007/09
Times Cited Count：7 Percentile：26.15(Radiology, Nuclear Medicine & Medical Imaging)Absorbed doses from main charged particle beams and charged-particle fragments have been measured with high accuracy for particle therapy but there are few reports for doses from neutron components produced as fragments. This study describes measurements on neutron dose produced by carbon beam, microdosimetric distributions of secondary neutrons produced by 290 MeV/nucleon carbon beams have been measured by using a tissue equivalent proportional counter (TEPC) at the Heavy Ion Medical Accelerator in Chiba (HIMAC) at the National Institute of Radiological Sciences (NIRS). The ratios of neutrons to charged particle fragments dominated to be 11 to 89 % in the absorbed doses at the side and below the faces of the acrylic phantom (300 mm height 
300 mm width 253 mm thickness).
