Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Initialising ...
Fujiwara, Satoru; Takezawa, Yasunori*; Sugimoto, Yasunobu*; Wakabayashi, Katsuzo*
Journal of Structural Biology, 167(1), p.25 - 35, 2009/07
Times Cited Count：2 Percentile：4.97(Biochemistry & Molecular Biology)In neutron scattering and diffraction, information on variations of scattering length density of molecules of interest can be extracted by the contrast variation technique. In order to explore the possibilities of this contrast variation technique in neutron fiber diffraction, neutron diffraction measurements of skeletal muscles were performed. The neutron fiber diffraction patterns of frog sartorius muscles were measured in the relaxed state and the rigor state, in various D
O concentrations. It was shown that there were variations in the scattering length density of the protein regions in the unit cell of the muscle structure. Analysis of the equatorial reflections showed that the phase information of these reflections is obtained, that the scattering length density is different between the thick filament region and the thin filament region, and that the density of the thick filament changes as the state of muscle changes from the relaxed state to the rigor state.
Fujiwara, Satoru; Takezawa, Yasunori*; Sugimoto, Yasunobu*; Wakabayashi, Katsuzo*
Journal of the Physical Society of Japan, Vol.70, Supplement A, p.408 - 410, 2001/05
no abstracts in English
Fujiwara, Satoru; Takezawa, Yasunori*; Sugimoto, Yasunobu*; Wakabayashi, Katsuzo*
no journal, ,
In neutron scattering and diffraction, information on internal fluctuations or a variation of scattering length density of molecules of interest can be extracted by the contrast variation technique. This contrast variation technique was applied to measurements of neutron fiber diffraction of muscles. The neutron fiber diffraction patterns of frog sartorius muscles were measured in the relaxed state and the rigor state, in various D2O concentrations. It was shown that there were variations in the scattering length density distribution in the unit cell of the muscle structure. Analysis of the equatorial reflections showed that the phase information of these reflections is obtained, that the density projected onto a plane perpendicular to the fiber axis of muscle is different between the thick filament region and the thin filament region, and that the projected density of the thick filament changes as the state of muscle changes from the relaxed state to the rigor state.
