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論文

Effect of ion beam irradiation for ${it Asclepias}$ species

小林 伸雄*; 佐々木 真一郎*; 田崎 啓介*; 中務 明*; 野澤 樹; 長谷 純宏; 鳴海 一成

JAEA-Review 2010-065, JAEA Takasaki Annual Report 2009, P. 67, 2011/01

${it Asclepias}$ is one of the perennial ornamentals native to Central America, using for cutting, potting and bed flowers. To obtain flower and/or plant form mutation, ${it Asclepias}$ seeds were irradiated with ion beams. Seed germination rate decreased over 100 Gy and any seeds could not germinate at 150 Gy. Survival rate of seedlings decreased below 50% at 50 Gy. In the case of ${it Asclepias}$ seeds, irradiation of 50 to 200 Gy is estimated to be optimal to generate mutants.

論文

Mutation induction in ${it Asclepias}$ using ion beam irradiation

小林 伸雄*; 加納 さやか*; 佐々木 真一郎*; 田崎 啓介*; 中務 明*; 野澤 樹; 長谷 純宏; 鳴海 一成

JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 77, 2009/12

${it Asclepias}$ is one of the perennial ornamentals native to central America, using for cutting, potting and bed flowers. For the purpose to obtain flower and/or plant form mutation, ${it Asclepias}$ seeds were irradiated with ion beams. Irradiated seeds were sowed in green house and germinated seedlings were transplanted into pots. After 10 months, these plants were planted in the field. Seed germination rate decreased from 200 Gy irradiation with increasing doses and any seeds could not germinate at 500 Gy. Survival rate of seedlings decreased below 50% at 150 Gy and 200 Gy, and all seedlings were molted at 250 Gy and more higher doses. In 50 Gy and higher doses irradiated seedlings, abnormal morphology of leaves (rounded apical leaf, branched leaf and narrow leaf) was observed and appearance of abnormal leaf was increased with dose up to 200 Gy.

論文

Mutation induction in azalea seedlings using ion beam irradiation

小林 伸雄*; 田崎 啓介*; 加納 さやか*; 坂本 咲子*; 中務 明*; 長谷 純宏; 鳴海 一成

JAEA-Review 2008-055, JAEA Takasaki Annual Report 2007, P. 70, 2008/11

本研究では、花色及び花姿の突然変異を誘導するために、ツツジ4品種(${it Rhododendron ripense}$, ${it R. japonicum}$, ${it R. wadanum}$及び${it R. degronianum}$ var. ${it okiense}$)の種子に220MeV炭素イオンビームを0から50Gy照射した。${it R. ripense}$${it R. japonicum}$については20Gy照射区まで生存し、また、${it R. wadanum}$${it R. degronianum}$ var. ${it okiense}$は30Gy照射区まで生存できた。発芽率は非照射区で最も高かったが、生存率は非照射区よりも低線量照射区の方が高かった。これは、イオンビーム照射による刺激効果によるものであろう。斑入りの葉を持つものが幾つかの苗木で観察された。矮性や発育阻害も観察された。

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