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Journal Articles

Structure and function of $$Delta$$1-tetrahydrocannabinolic acid (THCA) synthase, the enzyme controlling the psychoactivity of ${it Cannabis sativa}$

Shoyama, Yoshinari*; Tamada, Taro; Kurihara, Kazuo; Takeuchi, Ayako*; Taura, Futoshi*; Arai, Shigeki; Blaber, M.*; Shoyama, Yukihiro*; Morimoto, Satoshi*; Kuroki, Ryota

Journal of Molecular Biology, 423(1), p.96 - 105, 2012/10

 Times Cited Count:79 Percentile:89.33(Biochemistry & Molecular Biology)

$$Delta$$1-tetrahydrocannabinolic acid (THCA) synthase catalyzes the oxidative cyclization of cannabigerolic acid (CBGA) into THCA, the precursor of the primary psychoactive agent $$Delta$$1-tetrahydrocannabinol in ${it Cannabis sativa}$. The structure-function relationship of THCA synthase was investigated by X-ray structure determination (2.75 ${AA}$ resolution) and mutational analysis. Specific amino acid residues were identified in the active site of THCA synthase that are involved in the oxidative cyclization of the CBGA substrate.

Journal Articles

Crystallization and preliminary X-ray diffraction studies of polyketide synthase-1 (PKS-1) from ${it Cannabis sativa}$

Taguchi, Chiho; Taura, Futoshi*; Tamada, Taro; Shoyama, Yoshinari; Shoyama, Yukihiro*; Tanaka, Hiroyuki*; Kuroki, Ryota; Morimoto, Satoshi*

Acta Crystallographica Section F, 64(3), p.217 - 220, 2008/03

 Times Cited Count:3 Percentile:36.12(Biochemical Research Methods)

Oral presentation

X-ray crystal structure of THCA synthase

Shoyama, Yoshinari*; Takeuchi, Ayako*; Taura, Futoshi*; Shoyama, Yukihiro*; Adachi, Motoyasu; Tamada, Taro; Kuroki, Ryota

no journal, , 

no abstracts in English

Oral presentation

Crystal structure of delta1-tetrahydrocannabinolic acid (THCA) synthase from cannabis sativa

Shoyama, Yoshinari; Tamada, Taro; Takeuchi, Ayako*; Taura, Futoshi*; Adachi, Motoyasu; Shoyama, Yukihiro*; Kuroki, Ryota; Morimoto, Satoshi*

no journal, , 

no abstracts in English

Oral presentation

Expression and purification of polyketide synthase from cannabis sativa

Taguchi, Chiho*; Taura, Futoshi*; Morimoto, Satoshi*; Shoyama, Yoshinari; Tamada, Taro; Kuroki, Ryota; Shoyama, Yukihiro*

no journal, , 

no abstracts in English

Oral presentation

Crystal structure of Delta1-tetrahydrocannabinolic acid synthase from ${it Cannabis sativa}$

Shoyama, Yoshinari; Tamada, Taro; Takeuchi, Ayako*; Taura, Futoshi*; Shoyama, Yukihiro*; Kuroki, Ryota; Morimoto, Satoshi*

no journal, , 

Oral presentation

Crystal structure of Delta1-tetrahydrocannabinolic acid synthase from ${it Cannabis sativa}$

Shoyama, Yoshinari; Tamada, Taro; Takeuchi, Ayako*; Taura, Futoshi*; Shoyama, Yukihiro*; Morimoto, Satoshi*; Kuroki, Ryota

no journal, , 

7 (Records 1-7 displayed on this page)
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