Yamaguchi, Mika; Hidaka, Akihide; Ikuta, Yuko; Murakami, Kenta*; Tomita, Akira*; Hirose, Hiroya*; Watanebe, Masanori*; Ueda, Kinichi*; Namaizawa, Ken*; Onose, Takatoshi*; et al.
JAEA-Review 2017-002, 60 Pages, 2017/03
Since 2010, IAEA has held the NEM School to develop future leaders who plan and manage nuclear energy utilization in their county. Since 2012, JAEA together with Japan Nuclear HRD Network, University of Tokyo, Japan Atomic Industrial Forum and JAIF International Cooperation Center have cohosted the school in Japan in cooperation with IAEA. Since then, the school has been held in Japan every year. In 2006, Japanese nuclear technology and experience, such as lessons learned from the Fukushima Daiichi Nuclear Power Plant accident, were provided to offer a unique opportunity for the participants to learn about particular cases in Japan. Through the school, we contributed to the internationalization of Japanese young nuclear professionals, development of nuclear human resource of other countries including nuclear newcomers, and enhanced cooperative relationship with IAEA. Additionally, collaborative relationship within the network was strengthened by organizing the school in Japan.
Tomita, Masanori*; Matsumoto, Hideki*; Funayama, Tomoo; Yokota, Yuichiro; Otsuka, Kensuke*; Maeda, Munetoshi*; Kobayashi, Yasuhiko
Life Sciences in Space Research, 6, p.36 - 43, 2015/07
A radiation-induced bystander response is generally known as a cellular response induced in unirradiated cell by receiving bystander signaling factors released from directly irradiated cells of a cell population. Bystander responses induced by high-LET heavy ions at low fluence are an important problem concerning the health of astronauts in the space environment. Here we set out NO-mediated bystander signal transductions induced by high-LET heavy-ion microbeam irradiation in normal human fibroblasts. Our findings suggest that Akt- and NF-B-dependent signaling pathway involving COX-2 plays an important role in the NO-mediated high-LET heavy-ion-induced bystander responses. Additionally, COX-2 may be used as a molecular marker of high-LET heavy-ion-induced bystander cells, which are distinguish form directly irradiated cells.
Matsumoto, Hideki*; Tomita, Masanori*; Otsuka, Kensuke*; Hatashita, Masanori*; Maeda, Munetoshi*; Funayama, Tomoo; Yokota, Yuichiro; Suzuki, Michiyo; Sakashita, Tetsuya; Ikeda, Hiroko; et al.
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 76, 2015/03
The objective of this project is to elucidate molecular mechanisms for the induction of radioadaptive response through radiation-induced bystander responses induced by irradiation with heavy ion microbeams in JAEA. We found that the adaptive response was induced by Ar (520 MeV Ar) microbeam-irradiation of a limited number of cells, followed by the broad beam-irradiation and that the adaptive response was almost completely suppressed by the addition of carboxy-PTIO, as a nitric oxide (NO) scavenger. In addition, we found several genes induced specifically and preferentially when radioadaptive response could be induced. We confirmed that expression was specifically induced only when radioadaptive response could be induced. Our findings strongly suggested that radioadaptive response can be induced by NO-mediated bystander responses evoked by irradiation with heavy ion microbeams.
Tomita, Masanori*; Matsumoto, Hideki*; Otsuka, Kensuke*; Funayama, Tomoo; Yokota, Yuichiro; Suzuki, Michiyo; Sakashita, Tetsuya; Kobayashi, Yasuhiko
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 77, 2015/03
Radiation-induced bystander responses are defined as responses in cells that have not been directly targeted by radiation but are in the neighborhood of cells that have been directly exposed. In this study, we aim to clarify a role of bystander response to sustain the homeostasis of damaged tissue using heavy-ion microbeams. We established the heavy-ion microbeam irradiation method to a 3D cultured human epidermis. Using this method, a viable cell rate of the 3D cultured human epidermis irradiated with 260 MeV Ne-ion microbeams or broadbeams was analyzed by the MTT method.
Watanabe, Masahisa; Tagawa, Akihiro; Umemiya, Noriko; Maruyama, Noboru; Yoshida, Mami; Kawase, Keiichi; Noguchi, Shinichi; Sakazume, Yoshinori; Watanabe, Masanori; Hiraga, Hayato; et al.
JAEA-Review 2014-028, 184 Pages, 2014/10
JAEA received technical proposals from private enterprise about techniques that can be used for decontamination work, and "Decontamination Technology Demonstrations Projects" was commissioned from the Ministry of the Environment to verifies the decontamination effect, economy feasibility, safety, and other factors. By the "FY 2013 Decontamination Technology Demonstrations Projects" JAEA carried out technical advice of demonstration test and evaluation of 11 technologies (e.g., decontamination of soils and green space and wastes and washing of fly ash).
Kobayashi, Yasuhiko; Funayama, Tomoo; Hamada, Nobuyuki*; Sakashita, Tetsuya; Konishi, Teruaki*; Imaseki, Hitoshi*; Yasuda, Keisuke*; Hatashita, Masanori*; Takagi, Keiichi*; Hatori, Satoshi*; et al.
Journal of Radiation Research, 50(Suppl.A), p.A29 - A47, 2009/03
Hirayama, Ryoichi*; Ito, Atsushi*; Tomita, Masanori*; Tsukada, Teruyo*; Yatagai, Fumio*; Noguchi, Miho; Matsumoto, Yoshitaka*; Kase, Yuki*; Ando, Koichi*; Okayasu, Ryuichi*; et al.
Radiation Research, 171(2), p.212 - 218, 2009/02
The biological effects of radiation originate principally in damages to DNA. DNA damages by X-rays as well as heavy ions are induced by a combination of direct and indirect actions. The contribution of indirect action in cell killing can be estimated from the maximum degree of protection by dimethylsulfoxide (DMSO), which suppresses indirect action without affecting direct action. Exponentially growing Chinese hamster V79 cells were exposed to high-LET radiations of 20 to 2106 keV/m in the presence or absence of DMSO and their survival was determined using a colony formation assay. The contribution of indirect action to cell killing decreased with increasing LET. However, the contribution did not reach zero even at very high LETs and was estimated to be 32% at an LET of 2106 keV/m. Therefore, even though the radiochemically estimated G value of OH radicals was nearly zero at an LET of 1000 keV/m, indirect action by OH radicals contributed to a substantial fraction of the biological effects of high-LET radiations. The RBE determined at a survival level of 10% increased with LET, reaching a maximum value of 2.88 at 200 keV/m, and decreased thereafter. When the RBE was estimated separately for direct action (RBE(D)) and indirect action (RBE(I)); both exhibited an LET dependence similar to that of the RBE, peaking at 200 keV/m. However, the peak value was much higher for RBE(D) (5.99) than RBE(I) (1.89). Thus direct action contributes more to the high RBE of high-LET radiations than indirect action does.
Matsumoto, Hideki*; Tomita, Masanori*; Otsuka, Kensuke*; Funayama, Tomoo; Kobayashi, Yasuhiko
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