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shuttle vectorSato, Katsuya; Tu, Z.*; Oba, Hirofumi*; Narumi, Issei
JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 81, 2009/12
no abstracts in English
Sato, Katsuya; Tu, Z.*; Oba, Hirofumi; Narumi, Issei
Plasmid, 62(1), p.1 - 9, 2009/07
Times Cited Count：11 Percentile：27.77(Genetics & Heredity)To develop new shuttle vectors for 
species, the nucleotide sequence of the small cryptic plasmid pUE30 from Deinococcus radiopugnans ATCC19172 was determined. The 2,467-bp plasmid possesses two open reading frames, one encoding 88 amino acid residues (Orf1) and the other encoding 501 amino acid residues (Orf2). The predicted amino acid sequence encoded by Orf1 exhibits similarity to the N-terminal regions of replication proteins encoded by 
-type plasmids of 
-proteobacteria. On the other hand, the predicted amino acid sequence encoded by Orf2 exhibits similarity to replication proteins encoded by plasmids of 
SARK and 
species. Hybrid plasmids consisting of pUE30 and pKatCAT5, which replicates in 
with a chloramphenicol resistance determinant, were shown to autonomously replicate in 
ATCC43672. Deletion analysis revealed that Orf2 was necessary for replication of the plasmids in . On the other hand, a DNA fragment encompassing the Orf1-coding region was involved in the instability of the plasmid in . An expression plasmid that possesses the minimal 
promoter was constructed, and a firefly luciferase gene was successfully expressed in . The host-vector system developed in this study should prove useful in the bioremediation of radioactive waste and for the investigation of DNA repair mechanisms.
Yamauchi, Emiko*; Watanabe, Ritsuko; Oikawa, Miyoko*; Fujimoto, Hirofumi*; Yamada, Akinori*; Saito, Kimiaki; Murakami, Masahiro*; Hashido, Kazuo*; Tsuchida, Kozo*; Takada, Naoko*; et al.
Journal of Insect Biotechnology and Sericology, 77(1), p.17 - 24, 2008/02
The frequency of single strand breaks (SSBs) occurring on both strands of the pBR322 plasmid DNA region flanked by a pair of primers used for polymerase chain reaction (PCR) amplifications was determined after irradiation with 
Cs 
-rays. We refined that real time PCR is suitable for the detection and quantitative analysis of SSBs caused by -ray irradiation. The utility of this approach was also supported by the comparison of the practical experimental data with the Monte Carlo simulation. The potential application of this PCR method for the detection of genomic DNA damage was also confirmed.
?Ling, E.*; Shirai, Koji*; Kanekatsu, Rensuke*; Kobayashi, Yasuhiko; Tu, Z.; Funayama, Tomoo; Watanabe, Hiroshi; Kiguchi, Kenji*
Journal of Insect Biotechnology and Sericology, 72(2), p.101 - 109, 2003/09
no abstracts in English
, after locally targeted irradiation with heavy ion beamsLing, E.*; Fukamoto, Kana*; Xu, S.*; Shirai, Koji*; Kanekatsu, Rensuke*; Kobayashi, Yasuhiko; Tu, Z.; Funayama, Tomoo; Watanabe, Hiroshi; Kiguchi, Kenji*
Journal of Insect Biotechnology and Sericology, 72(2), p.95 - 100, 2003/09
no abstracts in English
Tu, Z.; Kobayashi, Yasuhiko; Kiguchi, Kenji*; Watanabe, Hiroshi
Nuclear Instruments and Methods in Physics Research B, 206, p.591 - 595, 2003/05
no abstracts in English
Tu, Z.; Kobayashi, Yasuhiko; Kiguchi, Kenji*; Watanabe, Hiroshi
Nuclear Instruments and Methods in Physics Research B, 206(1-4), p.591 - 595, 2003/05
Times Cited Count：0 Percentile：0.00(Instruments & Instrumentation)no abstracts in English
Tu, Z.; Kobayashi, Yasuhiko; Kiguchi, Kenji*; Watanabe, Hiroshi; Yamamoto, Kazuo*
Journal of Radiation Research, 43(3), p.269 - 275, 2002/11
Times Cited Count：11 Percentile：31.68(Biology)no abstracts in English
Tu, Z.; Kobayashi, Yasuhiko; Kiguchi, Kenji*; Watanabe, Hiroshi
Radiation and Environmental Biophysics, 41(3), p.231 - 234, 2002/08
Times Cited Count：5 Percentile：16.52(Biology)no abstracts in English
, by localized irradiation of heavy ion microbeamsKiguchi, Kenji*; Kinjo, Y.*; Masahashi, K.*; Tu, Z.; Tamura, Hajime*; Shirai, K.*; Kanekatsu, Rensuke*; Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi
JAERI-Review 2000-024, TIARA Annual Report 1999, p.51 - 53, 2000/10
no abstracts in English
Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi; Yamamoto, Kazuo*; Yamasaki, Shuhei*; Tu, Z. L.*; Kinjo, Y.*; Kiguchi, Kenji*
Proceedings of 11th International Congress of Radiation Research (ICRR-11), 2, p.182 - 186, 2000/00
no abstracts in English
; Morphological changes of the nuclei and cells after spot irradiationKiguchi, Kenji*; Shima, T.*; Kinjo, Y.*; Tu, Z. L.*; Yamasaki, Shuhei*; Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi
JAERI-Review 99-025, TIARA Annual Report 1998, p.53 - 55, 1999/10
no abstracts in English
Tu, Z. L.*; Yamasaki, Shuhei*; Shirai, Koji*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi
Nihon Sanshigaku Zasshi, 68(6), p.443 - 453, 1999/00
no abstracts in English
Tu, Z. L.*; Shirai, Koji*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Kobayashi, Yasuhiko; Taguchi, Mitsumasa; Watanabe, Hiroshi
Nihon Sanshigaku Zasshi, 68(6), p.491 - 500, 1999/00
no abstracts in English
Sato, Katsuya; Oba, Hirofumi*; Tu, Z.*; Narumi, Issei
no journal, ,
no abstracts in English
Sato, Katsuya; Oba, Hirofumi*; Tu, Z.*; Narumi, Issei
no journal, ,
To develop new shuttle vectors for species, the nucleotide sequence of the small cryptic plasmid pUE30 from 
was determined. The 2,467-bp plasmid possesses two open reading frames (Orf1 and Orf2). Hybrid plasmids consisting of pUE30 and pKatCAT5, which replicates in with a chloramphenicol resistance determinant, were shown to autonomously replicate in . Deletion analysis revealed that Orf2 was necessary for replication of the plasmids in . An expression plasmid that possesses the minimal promoter was constructed, and a firefly luciferase gene was successfully expressed in . The host-vector system developed in this study should prove useful in the bioremediation of radioactive waste and for the investigation of DNA repair mechanisms.
