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L. irradiated by 
raysKitano, Sayaka*; Miyagi, Atsuko*; Ono, Yutaka; Hase, Yoshihiro; Narumi, Issey*; Yamaguchi, Masatoshi*; Uchimiya, Hirofumi*; Kawai, Maki*
Metabolomics, 11(1), p.134 - 142, 2015/02
Times Cited Count：8 Percentile：25.41(Endocrinology & Metabolism)-ray irradiation on oxalate metabolism in L.Kitano, Sayaka*; Miyagi, Atsuko*; Ono, Yutaka; Hase, Yoshihiro; Narumi, Issey*; Uchimiya, Hirofumi*; Kawai, Maki*
JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 108, 2014/03
ray irradiation on L.Kitano, Sayaka*; Miyagi, Atsuko*; Ono, Yutaka; Hase, Yoshihiro; Narumi, Issei; Uchimiya, Hirofumi*; Kawai, Maki*
JAEA-Review 2012-046, JAEA Takasaki Annual Report 2011, P. 101, 2013/01
Nakasone, Akari*; Fujiwara, Masayuki*; Fukao, Yoichiro*; Biswas, K.; Rahman, A.*; Yamada, Maki*; Narumi, Issei; Uchimiya, Hirofumi*; Ono, Yutaka
Plant Physiology, 160(1), p.93 - 105, 2012/09
Times Cited Count：12 Percentile：38.62(Plant Sciences)
Nakasone, Akari; Kawai, Maki*; Kiyosue, Tomohiro*; Narumi, Issei; Uchimiya, Hirofumi*; Ono, Yutaka
JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 65, 2009/12
potentially mediates the response to synthetic auxin, 2,4-dichlorophenoxyacetic acid, in Nakasone, Akari; Yamada, Maki*; Kiyosue, Tomohiro*; Narumi, Issei; Uchimiya, Hirofumi*; Ono, Yutaka
Journal of Plant Physiology, 166(12), p.1307 - 1313, 2009/08
Times Cited Count：4 Percentile：13.07(Plant Sciences)
-chlorophenoxyisobutyric acid reveals that 
, a gene encoding a DCN1-like protein, regulates responses to the synthetic auxin 2,4-dichlorophenoxyacetic acid in Arabidopsis rootsBiswas, K. K.*; Oura, Chiharu*; Higuchi, Kanako*; Miyazaki, Yuji*; Nguyen, V. V.*; Rahman, A.*; Uchimiya, Hirofumi*; Kiyosue, Tomohiro*; Koshiba, Tomokazu*; Tanaka, Atsushi; et al.
Plant Physiology, 145(3), p.773 - 785, 2007/11
Times Cited Count：37 Percentile：66.14(Plant Sciences)We screened mutants for root growth resistance to a putative antiauxin, PCIB, which inhibits auxin action by interfering the upstream auxin signaling events. Eleven PCIB-resistant mutants were obtained. Genetic mapping indicates that the mutations are located in at least 5 independent loci including two known auxin-related loci, 
and 
. 
mutants (
s) 
, 
and 
were also resistant to 2,4-D as shown by a root growth assay. Positional cloning of revealed that the gene encodes a protein with a domain of unknown function (DUF298), which has not previously been implicated in auxin signaling. The protein has a putative nuclear localization signal and shares homology with the DCN-1 protein through the DUF298 domain. The results also indicate that PCIB can facilitate the identification of factors involved in auxin or auxin-related signaling.
Rahman, A.*; Nakasone, Akari*; Chhun, T.*; Oura, Chiharu*; Biswas, K. K.*; Uchimiya, Hirofumi*; Tsurumi, Seiji*; Baskin, T. I.*; Tanaka, Atsushi; Ono, Yutaka
Plant Journal, 47(5), p.788 - 801, 2006/09
Times Cited Count：33 Percentile：59.9(Plant Sciences)2,4-D, a chemical analogue of IAA, is widely used as a growth regulator and exogenous source of auxin. It is believed that they share a common response pathway. Here, we show that a mutant, 
(
) is resistant to 2,4-D, yet nevertheless responds like the wild type to IAA. That the mutation alters 2,4-D responsiveness specifically was confirmed by analysis of GUS expression in the
and 
backgrounds, as well as by real-time PCR quantification of 
expression. Complementation and RNAi experiments identified a gene that confers 2,4-D responsiveness. The gene encodes a 
with unknown function and present in plants, animals, and invertebrates. These results suggest that SMAP1 is a regulatory component that mediates responses to 2,4-D and that responsiveness to 2,4-D and IAA are partially distinct.
-chlorophenoxyisobutyric acid impairs auxin response in arabidopsis rootOno, Yutaka; Oura, Chiharu*; Rahman, A.; Aspuria, E. T.; Hayashi, Kenichiro*; Tanaka, Atsushi; Uchimiya, Hirofumi*
Plant Physiology, 133(3), p.1135 - 1147, 2003/11
Times Cited Count：132 Percentile：92.45(Plant Sciences)PCIB (-chlorophenoxyisobutyric acid) is known as a putative antiauxin and is widely used to inhibit auxin action, although the mechanism of PCIB-mediated inhibition of auxin action is not characterized very well at molecular level. In the present work, we showed that PCIB inhibited BA::GUS expression induced by IAA, 2,4-D and NAA. PCIB also inhibited auxin dependent DR5::GUS expression. RNA hybridization and quantitative RT-PCR analyses suggested that PCIB reduced auxin-induced accumulation of transcripts of 
genes. In addition, PCIB relieved the reduction of GUS activity in 
transgenic line in which auxin inhibits GUS activity by promoting degradation of the AXR3NT-GUS fusion protein. Physiological analysis revealed that PCIB inhibited lateral root production, gravitropic response of roots and growth of primary roots. These results suggest that PCIB impairs auxin signaling pathway by regulating Aux/IAA protein stability, and thereby affects the auxin-regulated Arabidopsis root physiology.
Aspuria, E. T.; Oura, Chiharu*; Chen, Q.*; Uchimiya, Hirofumi; Ono, Yutaka
Plant Cell Reports, 21(1), p.52 - 57, 2002/07
Times Cited Count：7 Percentile：17.54(Plant Sciences)no abstracts in English
Ono, Yutaka; Oura, Chiharu*; Uchimiya, Hirofumi
Annals of Botany, 89(1), p.77 - 82, 2002/01
Times Cited Count：10 Percentile：24.54(Plant Sciences)no abstracts in English
Oura, Chiharu*; Aspuria, E. T.; Ono, Yutaka; Hase, Yoshihiro; Kobayashi, Yasuhiko; Uchimiya, Hirofumi
JAERI-Review 2001-039, TIARA Annual Report 2000, p.67 - 69, 2001/11
no abstracts in English
genesLiu, J.; Oura, Chiharu*; Aspuria, E. T.; Ono, Yutaka; Uchimiya, Hirofumi
Chinese Science Bulletin, 46(19), p.1642 - 1645, 2001/10
Times Cited Count：1 Percentile：1.82(Multidisciplinary Sciences)no abstracts in English
Kawai, Maki; Kobayashi, Yasuhiko; Hirata, Aiko*; Ono, Yutaka; Watanabe, Hiroshi; Uchimiya, Hirofumi
Plant Biotechnology, 17(4), p.305 - 308, 2000/12
no abstracts in English
Kawai, Maki; Pan, L.*; Reed, J. C.*; Uchimiya, Hirofumi
FEBS Letters, 464(3), p.143 - 147, 1999/12
Times Cited Count：125 Percentile：93.25(Biochemistry & Molecular Biology)no abstracts in English
Kawai, Maki; Kobayashi, Yasuhiko; Ono, Yutaka; Watanabe, Hiroshi; Uchimiya, Hirofumi
JAERI-Review 99-025, TIARA Annual Report 1998, P. 56, 1999/10
no abstracts in English
, a gene encoding small acidic protein revealed by mutant screening with antiauxin is involved in 2,4-D sensitivity but not IAA sensitivity in arabidopsis rootsOno, Yutaka; Rahman, A.*; Nakasone, Hikari; Chhun, T.*; Uchimiya, Hirofumi*; Tsurumi, Seiji*; Tanaka, Atsushi
no journal, ,
no abstracts in English
Nakasone, Akari; Kawai, Maki*; Narumi, Issei; Uchimiya, Hirofumi*; Ono, Yutaka
no journal, ,
The 
() gene is recently identified as the factor that mediates the synthetic auxin 2,4-D response in root. SMAP1 has the conserved phenylalanine and asparagic acid (F/D) domain, which is highly conserved in both plants and animals. Introduction of 
to recovered 2,4-D sensitivity of while that of 
did not. Using anti-GFP-MicroBeads, the proteins that form complexes with SMAP1-GFP but not with SMAP1
F/D-GFP were pulled down from the extract and identified by MS analysis. The identified peptide sequences indicated that SMAP1-GFP binds to COP9 Signalosome (CSN). The results implied that SMAP1 regulates 2,4-D response by interacting to CSN through F/D region.
Nakasone, Akari; Uchimiya, Hirofumi*; Narumi, Issei; Ono, Yutaka; Kawai, Maki*; Kiyosue, Tomohiro*
no journal, ,
no abstracts in English
and that function in the 2,4-D signal transduction pathway in Ono, Yutaka; Nakasone, Akari; Uchimiya, Hirofumi*; Narumi, Issei
no journal, ,
The () gene is responsible for the anti-auxin resistant mutation, , and encodes a factor that mediates the synthetic auxin 2,4-D response. To gain an insight into the function of SMAP1, the mutant was crossed with known auxin-related mutants. The double mutants, 
, 
, and 
showed more resistant to 2,4-D than the corresponding single mutants in root growth assay. On the other hand, the 
double mutants showed severe morphological defects with a lack of root meristem formation. The overexpression of fusion gene under 35S promoter relieved pleiotropic morphological phenotypes of 
such as dwarf, multiple shoots, altered flower structure, low fertility and reduced auxin response. The results suggested that the function of is tightly linked to that of in genetic level.
