Metabolic alterations in leaves of oxalate-rich plant L. irradiated by rays

Kitano, Sayaka*; Miyagi, Atsuko*; Ono, Yutaka; Hase, Yoshihiro; Narumi, Issey*; Yamaguchi, Masatoshi*; Uchimiya, Hirofumi*; Kawai, Maki*

Metabolomics, 11(1), p.134 - 142, 2015/02

https://doi.org/10.1007/s11306-014-0684-4

Effect of ray irradiation on L.

Kitano, Sayaka*; Miyagi, Atsuko*; Ono, Yutaka; Hase, Yoshihiro; Narumi, Issei; Uchimiya, Hirofumi*; Kawai, Maki*

JAEA-Review 2012-046, JAEA Takasaki Annual Report 2011, P. 101, 2013/01

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2012-046.pdf

SMAP2 protein potentially mediates the response to synthetic auxin, 2,4-D, in

Nakasone, Akari; Kawai, Maki*; Kiyosue, Tomohiro*; Narumi, Issei; Uchimiya, Hirofumi*; Ono, Yutaka

JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 65, 2009/12

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2009-041.pdf

Genetic characterization of mutants resistant to the antiauxin -chlorophenoxyisobutyric acid reveals that , a gene encoding a DCN1-like protein, regulates responses to the synthetic auxin 2,4-dichlorophenoxyacetic acid in Arabidopsis roots

Biswas, K. K.*; Oura, Chiharu*; Higuchi, Kanako*; Miyazaki, Yuji*; Nguyen, V. V.*; Rahman, A.*; Uchimiya, Hirofumi*; Kiyosue, Tomohiro*; Koshiba, Tomokazu*; Tanaka, Atsushi; et al.

Plant Physiology, 145(3), p.773 - 785, 2007/11

https://doi.org/10.1104/pp.107.104844

We screened mutants for root growth resistance to a putative antiauxin, PCIB, which inhibits auxin action by interfering the upstream auxin signaling events. Eleven PCIB-resistant mutants were obtained. Genetic mapping indicates that the mutations are located in at least 5 independent loci including two known auxin-related loci, and . mutants (s) , and were also resistant to 2,4-D as shown by a root growth assay. Positional cloning of revealed that the gene encodes a protein with a domain of unknown function (DUF298), which has not previously been implicated in auxin signaling. The protein has a putative nuclear localization signal and shares homology with the DCN-1 protein through the DUF298 domain. The results also indicate that PCIB can facilitate the identification of factors involved in auxin or auxin-related signaling.

-chlorophenoxyisobutyric acid impairs auxin response in arabidopsis root

Ono, Yutaka; Oura, Chiharu*; Rahman, A.; Aspuria, E. T.; Hayashi, Kenichiro*; Tanaka, Atsushi; Uchimiya, Hirofumi*

Plant Physiology, 133(3), p.1135 - 1147, 2003/11

https://doi.org/10.1104/pp.103.027847

PCIB (-chlorophenoxyisobutyric acid) is known as a putative antiauxin and is widely used to inhibit auxin action, although the mechanism of PCIB-mediated inhibition of auxin action is not characterized very well at molecular level. In the present work, we showed that PCIB inhibited BA::GUS expression induced by IAA, 2,4-D and NAA. PCIB also inhibited auxin dependent DR5::GUS expression. RNA hybridization and quantitative RT-PCR analyses suggested that PCIB reduced auxin-induced accumulation of transcripts of genes. In addition, PCIB relieved the reduction of GUS activity in transgenic line in which auxin inhibits GUS activity by promoting degradation of the AXR3NT-GUS fusion protein. Physiological analysis revealed that PCIB inhibited lateral root production, gravitropic response of roots and growth of primary roots. These results suggest that PCIB impairs auxin signaling pathway by regulating Aux/IAA protein stability, and thereby affects the auxin-regulated Arabidopsis root physiology.

Expression pattern of Aux/IAA genes in the iaa3/shy2-1D mutant of arabidopsis thaliana (L.)

Ono, Yutaka; Oura, Chiharu*; Uchimiya, Hirofumi

Annals of Botany, 89(1), p.77 - 82, 2002/01

https://doi.org/10.1093/aob/mcf007

no abstracts in English

Application of real-time RT-PCR quantification to evaluate differential expression of genes

Liu, J.; Oura, Chiharu*; Aspuria, E. T.; Ono, Yutaka; Uchimiya, Hirofumi

Chinese Science Bulletin, 46(19), p.1642 - 1645, 2001/10

https://doi.org/10.1007/BF02900626

no abstracts in English

Evolutionally conserved plant homologue of the Bax Inhibitor-1 (BI-1) gene capable of suppressing Bax-induced cell death in yeast

Kawai, Maki; Pan, L.*; Reed, J. C.*; Uchimiya, Hirofumi

FEBS Letters, 464(3), p.143 - 147, 1999/12

https://doi.org/10.1016/S0014-5793(99)01695-6

no abstracts in English

, a gene encoding small acidic protein revealed by mutant screening with antiauxin is involved in 2,4-D sensitivity but not IAA sensitivity in arabidopsis roots

Ono, Yutaka; Rahman, A.*; Nakasone, Hikari; Chhun, T.*; Uchimiya, Hirofumi*; Tsurumi, Seiji*; Tanaka, Atsushi

no journal, , 

no abstracts in English

SMAP1 that functions in 2,4-D response interacts with COP9 Signalosome

Nakasone, Akari; Kawai, Maki*; Narumi, Issei; Uchimiya, Hirofumi*; Ono, Yutaka

no journal, , 

The () gene is recently identified as the factor that mediates the synthetic auxin 2,4-D response in root. SMAP1 has the conserved phenylalanine and asparagic acid (F/D) domain, which is highly conserved in both plants and animals. Introduction of to recovered 2,4-D sensitivity of while that of did not. Using anti-GFP-MicroBeads, the proteins that form complexes with SMAP1-GFP but not with SMAP1F/D-GFP were pulled down from the extract and identified by MS analysis. The identified peptide sequences indicated that SMAP1-GFP binds to COP9 Signalosome (CSN). The results implied that SMAP1 regulates 2,4-D response by interacting to CSN through F/D region.