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Journal Articles

Metabolic alterations in leaves of oxalate-rich plant ${it Rumex obtusifolius}$ L. irradiated by $$gamma$$ rays

Kitano, Sayaka*; Miyagi, Atsuko*; Ono, Yutaka; Hase, Yoshihiro; Narumi, Issey*; Yamaguchi, Masatoshi*; Uchimiya, Hirofumi*; Kawai, Maki*

Metabolomics, 11(1), p.134 - 142, 2015/02

 Times Cited Count:8 Percentile:29.74(Endocrinology & Metabolism)

Journal Articles

Effects of $$gamma$$-ray irradiation on oxalate metabolism in ${it Rumex obtusifolius}$ L.

Kitano, Sayaka*; Miyagi, Atsuko*; Ono, Yutaka; Hase, Yoshihiro; Narumi, Issey*; Uchimiya, Hirofumi*; Kawai, Maki*

JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 108, 2014/03

Journal Articles

Effect of $$gamma$$ ray irradiation on ${it Rumex obtusifolius}$ L.

Kitano, Sayaka*; Miyagi, Atsuko*; Ono, Yutaka; Hase, Yoshihiro; Narumi, Issei; Uchimiya, Hirofumi*; Kawai, Maki*

JAEA-Review 2012-046, JAEA Takasaki Annual Report 2011, P. 101, 2013/01

Journal Articles

SMALL ACIDIC PROTEIN 1 acts with RUB modification components the COP9 signalosome and AXR1 to regulate growth and development of ${it Arabidopsis}$

Nakasone, Akari*; Fujiwara, Masayuki*; Fukao, Yoichiro*; Biswas, K.; Rahman, A.*; Yamada, Maki*; Narumi, Issei; Uchimiya, Hirofumi*; Ono, Yutaka

Plant Physiology, 160(1), p.93 - 105, 2012/09

 Times Cited Count:11 Percentile:40.26(Plant Sciences)

Journal Articles

SMAP2 protein potentially mediates the response to synthetic auxin, 2,4-D, in ${it Arabidopsis thaliana}$

Nakasone, Akari; Kawai, Maki*; Kiyosue, Tomohiro*; Narumi, Issei; Uchimiya, Hirofumi*; Ono, Yutaka

JAEA-Review 2009-041, JAEA Takasaki Annual Report 2008, P. 65, 2009/12

Journal Articles

A Gene encoding ${it SMALL ACIDIC PROTEIN 2}$ potentially mediates the response to synthetic auxin, 2,4-dichlorophenoxyacetic acid, in ${it Arabidopsis thaliana}$

Nakasone, Akari; Yamada, Maki*; Kiyosue, Tomohiro*; Narumi, Issei; Uchimiya, Hirofumi*; Ono, Yutaka

Journal of Plant Physiology, 166(12), p.1307 - 1313, 2009/08

 Times Cited Count:4 Percentile:14.38(Plant Sciences)

Journal Articles

Genetic characterization of mutants resistant to the antiauxin ${it p}$-chlorophenoxyisobutyric acid reveals that ${it AAR3}$, a gene encoding a DCN1-like protein, regulates responses to the synthetic auxin 2,4-dichlorophenoxyacetic acid in Arabidopsis roots

Biswas, K. K.*; Oura, Chiharu*; Higuchi, Kanako*; Miyazaki, Yuji*; Nguyen, V. V.*; Rahman, A.*; Uchimiya, Hirofumi*; Kiyosue, Tomohiro*; Koshiba, Tomokazu*; Tanaka, Atsushi; et al.

Plant Physiology, 145(3), p.773 - 785, 2007/11

 Times Cited Count:34 Percentile:66.45(Plant Sciences)

We screened mutants for root growth resistance to a putative antiauxin, PCIB, which inhibits auxin action by interfering the upstream auxin signaling events. Eleven PCIB-resistant mutants were obtained. Genetic mapping indicates that the mutations are located in at least 5 independent loci including two known auxin-related loci, ${it TIR1}$ and ${it AtCUL1}$. ${it antiauxin-resistant}$ mutants (${it aar}$s) ${it aar3-1}$, ${it aar4}$ and ${it aar5}$ were also resistant to 2,4-D as shown by a root growth assay. Positional cloning of ${it aar3-1}$ revealed that the ${it AAR3}$ gene encodes a protein with a domain of unknown function (DUF298), which has not previously been implicated in auxin signaling. The protein has a putative nuclear localization signal and shares homology with the DCN-1 protein through the DUF298 domain. The results also indicate that PCIB can facilitate the identification of factors involved in auxin or auxin-related signaling.

Journal Articles

A Small acidic protein 1 (SMAP1) mediates responses of the arabidopsis root to the synthetic auxin 2,4-dichlorophenoxyacetic acid

Rahman, A.*; Nakasone, Akari*; Chhun, T.*; Oura, Chiharu*; Biswas, K. K.*; Uchimiya, Hirofumi*; Tsurumi, Seiji*; Baskin, T. I.*; Tanaka, Atsushi; Ono, Yutaka

Plant Journal, 47(5), p.788 - 801, 2006/09

 Times Cited Count:33 Percentile:62.81(Plant Sciences)

2,4-D, a chemical analogue of IAA, is widely used as a growth regulator and exogenous source of auxin. It is believed that they share a common response pathway. Here, we show that a mutant, ${it antiauxin resistant1}$ (${it aar1}$) is resistant to 2,4-D, yet nevertheless responds like the wild type to IAA. That the ${it aar1}$ mutation alters 2,4-D responsiveness specifically was confirmed by analysis of GUS expression in the${it DR5:GUS}$ and ${it HS:AXR3NT-GUS}$ backgrounds, as well as by real-time PCR quantification of ${it IAA11}$ expression. Complementation and RNAi experiments identified a gene that confers 2,4-D responsiveness. The gene encodes a ${it small, acidic protein 1}$with unknown function and present in plants, animals, and invertebrates. These results suggest that SMAP1 is a regulatory component that mediates responses to 2,4-D and that responsiveness to 2,4-D and IAA are partially distinct.

Journal Articles

${it p}$-chlorophenoxyisobutyric acid impairs auxin response in arabidopsis root

Ono, Yutaka; Oura, Chiharu*; Rahman, A.; Aspuria, E. T.; Hayashi, Kenichiro*; Tanaka, Atsushi; Uchimiya, Hirofumi*

Plant Physiology, 133(3), p.1135 - 1147, 2003/11

 Times Cited Count:122 Percentile:92.42(Plant Sciences)

PCIB (${it p}$-chlorophenoxyisobutyric acid) is known as a putative antiauxin and is widely used to inhibit auxin action, although the mechanism of PCIB-mediated inhibition of auxin action is not characterized very well at molecular level. In the present work, we showed that PCIB inhibited BA::GUS expression induced by IAA, 2,4-D and NAA. PCIB also inhibited auxin dependent DR5::GUS expression. RNA hybridization and quantitative RT-PCR analyses suggested that PCIB reduced auxin-induced accumulation of transcripts of ${it Aux/IAA}$ genes. In addition, PCIB relieved the reduction of GUS activity in ${it HS::AXR3NT-GUS}$ transgenic line in which auxin inhibits GUS activity by promoting degradation of the AXR3NT-GUS fusion protein. Physiological analysis revealed that PCIB inhibited lateral root production, gravitropic response of roots and growth of primary roots. These results suggest that PCIB impairs auxin signaling pathway by regulating Aux/IAA protein stability, and thereby affects the auxin-regulated Arabidopsis root physiology.

Journal Articles

GFP accumulation controlled by an auxin-responsive promoter as a non-destructive assay to monitor early auxin response

Aspuria, E. T.; Oura, Chiharu*; Chen, Q.*; Uchimiya, Hirofumi; Ono, Yutaka

Plant Cell Reports, 21(1), p.52 - 57, 2002/07

 Times Cited Count:7 Percentile:18.59(Plant Sciences)

no abstracts in English

Journal Articles

Expression pattern of Aux/IAA genes in the iaa3/shy2-1D mutant of arabidopsis thaliana (L.)

Ono, Yutaka; Oura, Chiharu*; Uchimiya, Hirofumi

Annals of Botany, 89(1), p.77 - 82, 2002/01

 Times Cited Count:10 Percentile:25.81(Plant Sciences)

no abstracts in English

Journal Articles

Genetic screening of antiauxin mutants in ${{it Arabidopsis Thaliana}}$

Oura, Chiharu*; Aspuria, E. T.; Ono, Yutaka; Hase, Yoshihiro; Kobayashi, Yasuhiko; Uchimiya, Hirofumi

JAERI-Review 2001-039, TIARA Annual Report 2000, p.67 - 69, 2001/11

no abstracts in English

Journal Articles

Application of real-time RT-PCR quantification to evaluate differential expression of ${it arabidopsis Aux/IAA}$ genes

Liu, J.; Oura, Chiharu*; Aspuria, E. T.; Ono, Yutaka; Uchimiya, Hirofumi

Chinese Science Bulletin, 46(19), p.1642 - 1645, 2001/10

 Times Cited Count:1 Percentile:1.85(Multidisciplinary Sciences)

no abstracts in English

Journal Articles

Ion beam as a noble tool to induce apoptosis-like cell death in roots of maie (zea. mays L.)

Kawai, Maki; Kobayashi, Yasuhiko; Hirata, Aiko*; Ono, Yutaka; Watanabe, Hiroshi; Uchimiya, Hirofumi

Plant Biotechnology, 17(4), p.305 - 308, 2000/12

no abstracts in English

Journal Articles

Evolutionally conserved plant homologue of the Bax Inhibitor-1 (BI-1) gene capable of suppressing Bax-induced cell death in yeast

Kawai, Maki; Pan, L.*; Reed, J. C.*; Uchimiya, Hirofumi

FEBS Letters, 464(3), p.143 - 147, 1999/12

 Times Cited Count:123 Percentile:93.48(Biochemistry & Molecular Biology)

no abstracts in English

Journal Articles

Induction of apoptosis in plant cells by ion beam irradiation

Kawai, Maki; Kobayashi, Yasuhiko; Ono, Yutaka; Watanabe, Hiroshi; Uchimiya, Hirofumi

JAERI-Review 99-025, TIARA Annual Report 1998, P. 56, 1999/10

no abstracts in English

Oral presentation

${it SMAP1}$, a gene encoding small acidic protein revealed by mutant screening with antiauxin is involved in 2,4-D sensitivity but not IAA sensitivity in arabidopsis roots

Ono, Yutaka; Rahman, A.*; Nakasone, Hikari; Chhun, T.*; Uchimiya, Hirofumi*; Tsurumi, Seiji*; Tanaka, Atsushi

no journal, , 

no abstracts in English

Oral presentation

SMAP1 that functions in 2,4-D response interacts with COP9 Signalosome

Nakasone, Akari; Kawai, Maki*; Narumi, Issei; Uchimiya, Hirofumi*; Ono, Yutaka

no journal, , 

The ${it Small Acidic Protein 1}$ (${it SMAP1}$) gene is recently identified as the factor that mediates the synthetic auxin 2,4-D response in ${it Arabidopsis}$ root. SMAP1 has the conserved phenylalanine and asparagic acid (F/D) domain, which is highly conserved in both plants and animals. Introduction of ${it SMAP1-GFP}$ to ${it aar1}$ recovered 2,4-D sensitivity of ${it aar1}$ while that of ${it SMAP1$Delta$F/D-GFP}$ did not. Using anti-GFP-MicroBeads, the proteins that form complexes with SMAP1-GFP but not with SMAP1$$Delta$$F/D-GFP were pulled down from the ${it Arabidopsis}$extract and identified by MS analysis. The identified peptide sequences indicated that SMAP1-GFP binds to COP9 Signalosome (CSN). The results implied that SMAP1 regulates 2,4-D response by interacting to CSN through F/D region.

Oral presentation

SMAP2 protein is involved in the sensitivity of Arabidopsis root to synthetic auxin 2,4-D

Nakasone, Akari; Uchimiya, Hirofumi*; Narumi, Issei; Ono, Yutaka; Kawai, Maki*; Kiyosue, Tomohiro*

no journal, , 

no abstracts in English

Oral presentation

Genetic interaction between ${it AXR1}$ and ${it SMAP1}$ that function in the 2,4-D signal transduction pathway in ${it Arabidopsis}$

Ono, Yutaka; Nakasone, Akari; Uchimiya, Hirofumi*; Narumi, Issei

no journal, , 

The ${it Small Acidic Protein 1}$ (${it SMAP1}$) gene is responsible for the anti-auxin resistant mutation, ${it aar1}$, and encodes a factor that mediates the synthetic auxin 2,4-D response. To gain an insight into the function of SMAP1, the ${it aar1}$ mutant was crossed with known auxin-related mutants. The double mutants, ${it aar1-1 tir1-1}$, ${it aar1-1 ecr1-1}$, and ${it aar1-1 aar3-2}$ showed more resistant to 2,4-D than the corresponding single mutants in root growth assay. On the other hand, the ${it axr1 aar1}$ double mutants showed severe morphological defects with a lack of root meristem formation. The overexpression of ${it SMAP1-GFP}$ fusion gene under 35S promoter relieved pleiotropic morphological phenotypes of ${it axr1-12}$ such as dwarf, multiple shoots, altered flower structure, low fertility and reduced auxin response. The results suggested that the function of ${it SMAP1}$ is tightly linked to that of ${it AXR1}$ in genetic level.

26 (Records 1-20 displayed on this page)