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Journal Articles

Screening of cesium-accumulating mutant of radioresistant bacterium ${it Deinococcus radiodurans}$ by ion beam breeding technology

Sato, Katsuya; Ueda, Ryoshiro*; Hase, Yoshihiro; Narumi, Issey*; Ono, Yutaka

JAEA-Review 2015-022, JAEA Takasaki Annual Report 2014, P. 100, 2016/02

https://doi.org/10.11484/jaea-review-2015-022

Journal Articles

Accumulation of cesium and its association with intracellular polyphosphate in ${it Deinococcus radiodurans}$

Ueda, Ryoshiro*; Sato, Katsuya; Hayashi, Hidenori*; Narumi, Issey*; Ono, Yutaka

JAEA-Review 2015-022, JAEA Takasaki Annual Report 2014, P. 101, 2016/02

https://doi.org/10.11484/jaea-review-2015-022

Journal Articles

Development of cesium-accumulating bacteria by ion beam breeding technology

Sato, Katsuya; Ueda, Ryoshiro; Hase, Yoshihiro; Narumi, Issey*; Ono, Yutaka

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 117, 2015/03

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2014-050.pdf#page=149

Journal Articles

Molecular analysis of polyphosphate biosynthesis-related genes in ${it Deinococcus radiodurans}$

Ueda, Ryoshiro; Sato, Katsuya; Hayashi, Hidenori*; Narumi, Issey*; Ono, Yutaka

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 118, 2015/03

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2014-050.pdf#page=150

Journal Articles

Genome analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding

Masubuchi, Takashi*; Hyuga, Hirokazu*; Ueda, Ryoshiro; Hayashi, Hidenori*; Ikenaga, Hiroshi*; Sato, Katsuya; Ono, Yutaka

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 123, 2015/03

https://jopss.jaea.go.jp/pdfdata/JAEA-Review-2014-050.pdf#page=155

Journal Articles

Mutation analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding

Masubuchi, Takashi*; Ueda, Ryoshiro; Kamiyama, Osamu*; Ikenaga, Hiroshi*; Sato, Katsuya; Narumi, Issey*

JAEA-Review 2013-059, JAEA Takasaki Annual Report 2012, P. 117, 2014/03

Oral presentation

Mutation analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding

Sato, Katsuya; Ueda, Ryoshiro; Masubuchi, Takashi*; Kamiyama, Osamu*; Hayashi, Hidenori*; Ikenaga, Hiroshi*; Narumi, Issey*; Tanaka, Atsushi

no journal, , 

We have previously obtained high ethyl caproate producing Sake yeasts "No.227" and "No.1333" by ion beam breeding. The fatty acid synthase genes (${it FAS1}$ and ${it FAS2}$) and the fatty acid esterase genes (${it EHT1}$ and ${it EEB1}$) might be involved in the higher production of ethyl caproate in the mutant strains. In this study, to identify the mutation sites of the mutant strains, each gene locus was sequenced. For the ${it EHT1}$, ${it EEB1}$ and ${it FAS1}$ loci, all the mutations found in mutant strains are nonsense mutations, suggesting that these mutations did not involved in the higher production of ethyl caproate. On the other hand, strains No.227 and No.1333 carried G/A and A/A mutations in ${it FAS2}$ gene at nucleotide position 3,748 as heterozygous and homozygous states, respectively. This result strongly suggested that the higher production of ethyl caproate in strains No.227 and No.1333 is attributed to a dominant mutation in ${it FAS2}$ gene.

Oral presentation

Good flavor Japanese sake generated by ion beam breeding technology

Sato, Katsuya; Masubuchi, Takashi*; Kamiyama, Osamu*; Ueda, Ryoshiro; Hayashi, Hidenori*; Ikenaga, Hiroshi*; Narumi, Issey*

no journal, , 

Sake is a useful tool for promoting local industry, but sake consumption has been decreasing year by year. In Gunma prefecture, the Gunma industrial technology center has playing a central role in breeding original sake yeast. Furthermore, for quickly corresponding with the needs from market, JAEA have tried breeding of sake yeast by ion beam breeding technology for development of distinctive sake yeast and its practical use while closely coordinating with the Gunma industrial technology center. As a result, the high ethyl caproate producing novel sake yeast were obtained.

Oral presentation

Mutation analysis of high ethyl caproate producing sake yeasts generated by ion beam breeding

Masubuchi, Takashi*; Kamiyama, Osamu*; Ueda, Ryoshiro; Ikenaga, Hiroshi*; Hayashi, Hidenori*; Sato, Katsuya; Narumi, Issey*

no journal, , 

We have previously obtained high ethyl caproate producing Sake yeasts "No.227" and "No.1333" by ion beam breeding. The fatty acid synthase genes (${it FAS1}$ and ${it FAS2}$) and the fatty acid esterase genes (${it EHT1}$ and ${it EEB1}$) might be involved in the higher production of ethyl caproate in the mutant strains. In this study, to identify the mutation sites of the mutant strains, each gene locus was sequenced. For the ${it EHT1}$, ${it EEB1}$ and ${it FAS1}$ loci, all the mutations found in mutant strains are nonsense mutations, suggesting that these mutations did not involved in the higher production of ethyl caproate. On the other hand, strains No.227 and No.1333 carried G/A and A/A mutations in ${it FAS2}$ gene at nucleotide position 3,748 as heterozygous and homozygous states, respectively. This result strongly suggested that the higher production of ethyl caproate in strains No.227 and No.1333 is attributed to a dominant mutation in ${it FAS2}$ gene.

Oral presentation

Development of cesium-accumulating bacteria by ion beam breeding technology

Sato, Katsuya; Ueda, Ryoshiro; Hase, Yoshihiro; Ono, Yutaka; Narumi, Issey*

no journal, , 

no abstracts in English

Oral presentation

Molecular analysis of polyphosphate biosynthesis-related genes in ${it Deinococcus radiodurans}$

Ueda, Ryoshiro; Sato, Katsuya; Hayashi, Hidenori*; Narumi, Issey*; Ono, Yutaka

no journal, , 

no abstracts in English

Oral presentation

Generation of cesium-accumulating mutant of radioresistant bacteria by ion beam breeding technology

Sato, Katsuya; Ueda, Ryoshiro; Hase, Yoshihiro; Ono, Yutaka; Narumi, Issey*

no journal, , 

no abstracts in English

Oral presentation

Polyphosphate production and cesium accumulation in the radioresistant bacterium ${it Deinococcus radiodurans}$

Ueda, Ryoshiro; Sato, Katsuya; Hayashi, Hidenori*; Narumi, Issey*; Ono, Yutaka

no journal, , 

no abstracts in English

Oral presentation

Screening of cesium-accumulating mutant of radioresistant bacterium ${it Deinococcus radiodurans}$ by ion beam breeding technology

Sato, Katsuya; Ueda, Ryoshiro*; Hase, Yoshihiro; Ono, Yutaka; Narumi, Issey*

no journal, , 

no abstracts in English

Oral presentation

Accumulation of cesium and its association with intracellular polyphosphate in ${it Deinococcus radiodurans}$

Ueda, Ryoshiro*; Sato, Katsuya; Ono, Yutaka; Hayashi, Hidenori*; Narumi, Issey*

no journal, , 

no abstracts in English

15 (Records 1-15 displayed on this page)
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