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Journal Articles

${it Desulfovibrio subterraneus}$ sp. nov., a mesophilic sulfate-reducing deltaproteobacterium isolated from a deep siliceous mudstone formation

Ueno, Akio*; Tamazawa, Satoshi*; Tamamura, Shuji*; Murakami, Takuma*; Kiyama, Tamotsu*; Inomata, Hidenori*; Amano, Yuki; Miyakawa, Kazuya; Tamaki, Hideyuki*; Naganuma, Takeshi*; et al.

International Journal of Systematic and Evolutionary Microbiology, 71(2), p.004683_1 - 004683_10, 2021/02

 Times Cited Count:6 Percentile:63.69(Microbiology)

A novel mesophilic sulfate-reducing bacterium, designated strain HN2$$^{rm T}$$, was isolated from groundwater sampled from the subsurface Miocene Wakkanai Formation located in Horonobe, Hokkaido, Japan. The cells were Gram-negative rods, with motility conferred by a single polar flagellum. The isolate expressed desulfoviridin, but no catalase or oxidase activities was detected. Strain HN2$$^{rm T}$$ grew in a temperature range of 5-43 $$^{circ}$$C (optimum, 35 $$^{circ}$$C) and in a pH range of 6.5-7.5 (optimum, pH 7.0-7.3). It used sulfate, thiosulfate, dimethyl sulfoxide, anthraquinone-2,6-disulfonate, Fe$$^{3+}$$, and manganese oxide as electron acceptors, but not elemental sulfur, nitrite, or nitrate. The bacterium showed very weak growth with sulfite as the electron acceptor. The strain fermented pyruvate and cysteine in the absence of sulfate, but not malate or succinate. The bacterium did not require NaCl, but tolerated up to 4% NaCl (w/v). Strain HN2$$^{rm T}$$ did not require vitamins. The G+C content of the genomic DNA was 56.66 mol%. A 16S rRNA gene sequence analysis showed that the closest recognized relative of strain HN2$$^{rm T}$$ is ${it Desulfovibrio psychrotolerans}$ JS1$$^{rm T}$$ (97.0% similarity). The average nucleotide identity (ANI) value between strain HN2$$^{rm T}$$ and D. ${it psychrotolerans}$ JS1$$^{rm T}$$ was 79.8%. Based on the phenotypic and molecular genetic evidence, the isolate is assigned to the new species ${it Desulfovibrio subterraneus}$ sp. nov. The type strain is HN2$$^{rm T}$$ (=DSM 101010$$^{rm T}$$ =NBRC 112213$$^{rm T}$$).

Journal Articles

2016 Professional Engineer (PE) test preparation course "Nuclear and Radiation Technical Disciplines"

Takahashi, Naoki; Yoshinaka, Kazuyuki; Harada, Akio; Yamanaka, Atsushi; Ueno, Takashi; Kurihara, Ryoichi; Suzuki, Soju; Takamatsu, Misao; Maeda, Shigetaka; Iseki, Atsushi; et al.

Nihon Genshiryoku Gakkai Homu Peji (Internet), 64 Pages, 2016/00

no abstracts in English

JAEA Reports

Mizunami Underground Research Laboratory Project, Annual report for fiscal year 2010

Kunimaru, Takanori; Mikake, Shinichiro; Nishio, Kazuhisa; Tsuruta, Tadahiko; Matsuoka, Toshiyuki; Ishibashi, Masayuki; Ueno, Takashi; Tokuyasu, Shingo; Daimaru, Shuji; Takeuchi, Ryuji; et al.

JAEA-Review 2012-020, 178 Pages, 2012/06

JAEA-Review-2012-020.pdf:33.16MB

Japan Atomic Energy Agency (JAEA) at Tono Geoscience Center (TGC) is pursuing a geoscientific research and development project namely the Mizunami Underground Research Laboratory (MIU) Project in crystalline rock environment in order to construct scientific and technological basis for geological disposal of High-level Radioactive Waste (HLW). The MIU Project has three overlapping phases: Surface-based Investigation phase (Phase I), Construction phase (Phase II), and Operation phase (Phase III). The MIU Project has been ongoing the Phase II. And Phase III started in 2010 fiscal year. This report shows the results of the investigation, construction and collaboration studies in fiscal year 2010, as a part of the Phase II based on the MIU Master Plan updated in 2002.

Journal Articles

Irradiation history of Itokawa regolith material deduced from noble gases in the Hayabusa samples

Nagao, Keisuke*; Okazaki, Ryuji*; Nakamura, Tomoki*; Miura, Yayoi*; Osawa, Takahito; Bajo, Kenichi*; Matsuda, Shintaro*; Ebihara, Mitsuru*; Ireland, T.*; Kitajima, Fumio*; et al.

Science, 333(6046), p.1128 - 1131, 2011/08

 Times Cited Count:125 Percentile:95.35(Multidisciplinary Sciences)

A steroid surface materials record regolith processes and a history of cosmic-ray irradiation. Noble gas isotopes in three rocky grains from Itokawa have been determined. High concentrations of solar He, Ne, and Ar, as high as those in lunar soils, are released at variable temperatures from each sample. The isotopic compositions are essentially identical to those of solar wind but distinguishable in He relative abundance. These noble gas characteristics can be explained by repeated implantation and preferential loss of solar He by removal of weathered He-rich rim on the grain surface through friction among regolith grains on Itokawa. Residence time of regolith materials on Itokawa is alculated to be shorter than 10 Myr, suggesting that regolith materials of small asteroids would escape easily to space.

JAEA Reports

The First loading fuel elements and power-up for JRR-2

JRR-2 Control Office; Kambara, Toyozo; Shoda, Katsuhiko; Hirata, Yutaka; Shoji, Tsutomu; Kohayakawa, Toru; Morozumi, Minoru; Kambayashi, Yuichiro; Shitomi, Hajimu; Kokanezawa, Takashi; et al.

JAERI 1027, 57 Pages, 1962/09

JAERI-1027.pdf:4.76MB

no abstracts in English

JAEA Reports

Critical experiments and characteristic measurement for JRR-2

JRR-2 Critical Experiments Group; Kambara, Toyozo; Shoda, Katsuhiko; Hirata, Yutaka; Shoji, Tsutomu; Kohayakawa, Toru; Morozumi, Minoru; Kambayashi, Yuichiro; Shitomi, Hajimu; Kokanezawa, Takashi; et al.

JAERI 1025, 62 Pages, 1962/03

JAERI-1025.pdf:4.6MB

no abstracts in English

Oral presentation

Mizunami Underground Research Laboratory Project; Hydrogeological study during the operation phase; Understanding of hydrogeological study on groundwater recovery experiment in tunnel

Beppu, Shinji; Onoe, Hironori; Kohashi, Akio; Ueno, Tetsuro; Takayasu, Kentaro; Takeuchi, Ryuji; Ishibashi, Masayuki; Sawada, Atsushi

no journal, , 

no abstracts in English

Oral presentation

Isolation and characterization of a novel fermentative bacterium in the phylum Bacteroidetes from a deep subsurface diatomaceous mudstone formation

Tamazawa, Satoshi*; Ueno, Akio*; Tamaki, Hideyuki*; Tamamura, Shuji*; Murakami, Takuma*; Kiyama, Tamotsu*; Inomata, Hidenori*; Miyakawa, Kazuya; Naganuma, Takeshi*; Kaneko, Katsuhiko*

no journal, , 

no abstracts in English

Oral presentation

Characterization of a novel fermentative iron-reducing Bacteroidetes bacterium isolated from deep subsurface diatomaceous mudstone formation

Tamazawa, Satoshi*; Ueno, Akio*; Tamaki, Hideyuki*; Tamamura, Shuji*; Murakami, Takuma*; Kiyama, Tamotsu*; Inomata, Hidenori*; Miyakawa, Kazuya; Naganuma, Takeshi*; Kaneko, Katsuhiko*

no journal, , 

no abstracts in English

Oral presentation

Analysis of microbial community structure in deep underground diatomaceous/siliceous mudstone formations and factors determining microbial community structure

Tamazawa, Satoshi*; Ueno, Akio*; Murakami, Takuma*; Miyakawa, Kazuya; Tamamura, Shuji*; Kiyama, Tamotsu*; Inomata, Hidenori*; Naganuma, Takeshi*; Kaneko, Katsuhiko*; Igarashi, Toshifumi*

no journal, , 

no abstracts in English

Oral presentation

A Novel bacterium, ${it Mangrovibacterium}$ sp. strain Z1-71, isolated from the terrestrial subsurface environment and its co-cultivation within a methanogenic archaeon

Ueno, Akio*; Tamazawa, Satoshi*; Tamamura, Shuji*; Murakami, Takuma*; Inomata, Hidenori*; Amano, Yuki; Miyakawa, Kazuya; Naganuma, Takeshi*; Igarashi, Toshifumi*

no journal, , 

no abstracts in English

Oral presentation

A Novel bacterium isolated from the terrestrial subsurface environment and its co-cultivation with a methanogenic archaeon

Ueno, Akio*; Sato, Kiyoshi*; Tamamura, Shuji*; Murakami, Takuma*; Inomata, Hidenori*; Tamazawa, Satoshi*; Amano, Yuki; Miyakawa, Kazuya; Naganuma, Takeshi*; Igarashi, Toshifumi*

no journal, , 

no abstracts in English

12 (Records 1-12 displayed on this page)
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