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Journal Articles

Neutron diffraction measurements of skeletal muscle using the contrast variation technique; Analysis of the equatorial diffraction patterns

Fujiwara, Satoru; Takezawa, Yasunori*; Sugimoto, Yasunobu*; Wakabayashi, Katsuzo*

Journal of Structural Biology, 167(1), p.25 - 35, 2009/07

 Times Cited Count:2 Percentile:5.84(Biochemistry & Molecular Biology)

In neutron scattering and diffraction, information on variations of scattering length density of molecules of interest can be extracted by the contrast variation technique. In order to explore the possibilities of this contrast variation technique in neutron fiber diffraction, neutron diffraction measurements of skeletal muscles were performed. The neutron fiber diffraction patterns of frog sartorius muscles were measured in the relaxed state and the rigor state, in various D$$_{2}$$O concentrations. It was shown that there were variations in the scattering length density of the protein regions in the unit cell of the muscle structure. Analysis of the equatorial reflections showed that the phase information of these reflections is obtained, that the scattering length density is different between the thick filament region and the thin filament region, and that the density of the thick filament changes as the state of muscle changes from the relaxed state to the rigor state.

Journal Articles

An Insight into the pathway of the amyloid fibril formation of hen egg white lysozyme obtained from a small-angle X-ray and neutron scattering study

Yonezawa, Yasushige*; Tanaka, Shimpei*; Kubota, Tomomi*; Wakabayashi, Katsuzo*; Yutani, Katsuhide*; Fujiwara, Satoru

Journal of Molecular Biology, 323(2), p.237 - 251, 2002/10

 Times Cited Count:71 Percentile:74.49(Biochemistry & Molecular Biology)

It is known that hen egg white lysozyme (HEWL) forms amyloid fibrils in highly concentrated ethanol solutions. In order to gain an insight into the mechanism of the amyloid fibril formation, the structures of HEWL in solutions of various protein and ethanol concentrations were investigated with small-angle X-ray and neutron scattering. It was shown that the structural states of HEWL were distinguished as the monomer state, the state of the dimer formation, the state of the protofilament formation, the protofilament state, and the state towards the formation of the amyloid fibrils. Circular dichroism measurements showed that the large changes in the secondary structures of HEWL occurred during the dimer formation. Structural characterization showed that the dimers had an elongated shape, the protofilaments were formed by stacking of the dimers with their long axis (nearly) perpendicular to the protofilament axis, and the changes of the structural states towards the amyloid fibril formation occurred via lateral association of the protofilaments.

Journal Articles

Neutron fiber diffraction of frog muscle with the contrast variation measurements

Fujiwara, Satoru; Takezawa, Yasunori*; Sugimoto, Yasunobu*; Wakabayashi, Katsuzo*

Journal of the Physical Society of Japan, Vol.70, Supplement A, p.408 - 410, 2001/05

no abstracts in English

Oral presentation

Neutron fiber diffraction measurements of muscle using the contrast variation technique

Fujiwara, Satoru; Takezawa, Yasunori*; Sugimoto, Yasunobu*; Wakabayashi, Katsuzo*

no journal, , 

In neutron scattering and diffraction, information on internal fluctuations or a variation of scattering length density of molecules of interest can be extracted by the contrast variation technique. This contrast variation technique was applied to measurements of neutron fiber diffraction of muscles. The neutron fiber diffraction patterns of frog sartorius muscles were measured in the relaxed state and the rigor state, in various D2O concentrations. It was shown that there were variations in the scattering length density distribution in the unit cell of the muscle structure. Analysis of the equatorial reflections showed that the phase information of these reflections is obtained, that the density projected onto a plane perpendicular to the fiber axis of muscle is different between the thick filament region and the thin filament region, and that the projected density of the thick filament changes as the state of muscle changes from the relaxed state to the rigor state.

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