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Tabata, Chihiro; Watanabe, Hirohito*; Shirasaki, Kenji*; Sunaga, Ayaki*; Fukuda, Takamitsu*; Li, D.*; Yamamura, Tomoo*
Journal of Molecular Structure, 1277, p.134870_1 - 134870_8, 2023/04
Times Cited Count:0 Percentile:0.00(Chemistry, Physical)Neutral and cationic U(IV) sandwiched phthalocyanine (Pc) complexes were prepared. The neutral species, UPc, was obtained by the reaction of UCl] and phthalonitrile, and the [UPc][BF] crystals were grown by electrolysis. The structures of the complexes were determined crystallographically. A U(IV) ion has two electrons, which carry a magnetic moment localized at the U sites, making the complex magnetically active. The magnetic susceptibility measurement of UPc revealed that the system was paramagnetic with local magnetic moments down to 2 K, but these magnetic moments were suppressed, possibly by a ligand field effect. The paramagnetism was also confirmed by the magnetization curve without hysteresis. The cationic complex, [UPc][BF]] crystalized in a tetragonal structure with the space group , in which the UPc molecules stacked along the tetragonal -axis. The structure was similar to that of [LnPc][BF]], but with distinct disorder in the stacking plane. The stabilities of the crystal and molecular structures and the electronic configurations of UPc and [UPc][BF]] were evaluated via the calculations that included the multiconfigurational nature of the actinide element.
Ueta, Shohei; Imai, Yoshiyuki; Watanabe, Masashi; Segawa, Tomoomi; Yan, X.
International Journal of Applied Ceramic Technology, 20(1), p.261 - 265, 2023/01
Times Cited Count:2 Percentile:25.30(Materials Science, Ceramics)Amano, Yuki; Watanabe, Koji; Masaki, Tomoo; Tashiro, Shinsuke; Abe, Hitoshi
JAEA-Technology 2016-012, 21 Pages, 2016/06
To contribute to safety evaluation of fire accident in fuel reprocessing plants, solvent extraction behavior of ruthenium, which could form volatile species, was investigated. Distribution ratios of ruthenium at fire accident conditions were obtained by extraction experiments with several solvent composition at different temperature as parameters. In order to investigate release behavior of ruthenium and europium at fire accident, release ratios of ruthenium and europium were also obtained by solvent combustion experiments.
Hattori, Yuya; Suzuki, Michiyo; Funayama, Tomoo; Kobayashi, Yasuhiko; Yokoya, Akinari; Watanabe, Ritsuko
Radiation Protection Dosimetry, 166(1-4), p.142 - 147, 2015/09
Times Cited Count:5 Percentile:39.50(Environmental Sciences)Cell-to-cell communication is one of the important factors to understand the mechanisms of radiation-induced responses such as radiation-induced bystander effects at low doses. In the present study, we propose simulation-based analyses of the intercellular signal transmissions between the individual cells in the cellular population. We developed the transmissions of two types of signals, i.e., X is transmitted via culture medium and Y is transmitted via gap junctions based on the diffusion equation. To observe the cell cycle as the response of cell induced by the signals, X and Y, we represented the cell cycle as a virtual clock including several check-point pathways and the cyclic process (G1, S, G2, M phases). The cellular population was divided into the grids (cells), and the signals and the clock were calculated for each grid. The signals, X, Y, were transmitted to the cells and stopped the clocks at the check points. Furthermore, the radiation was modeled as the radiation signal, Z, which affected the clock and the signals, X and Y. We input the radiation signal, Z, to specific cells, and simulated the behaviors of the clock of each cell and signals, X and Y. We will discuss the usefulness of our model for investigating the mechanisms of radiation-induced responses of the cell cycle via cell-to-cell communications.
Tanaka, Shingo*; Yokota, Hideharu; Ono, Hirokazu; Nakayama, Masashi; Fujita, Tomoo; Takiya, Hiroaki*; Watanabe, Naoko*; Kozaki, Tamotsu*
Proceedings of 23rd International Conference on Nuclear Engineering (ICONE-23) (DVD-ROM), 6 Pages, 2015/05
Wada, Seiichi*; Ando, Tatsuhiko*; Watanabe, Aya*; Kakizaki, Takehiko*; Natsuhori, Masahiro*; Funayama, Tomoo; Sakashita, Tetsuya; Yokota, Yuichiro; Kobayashi, Yasuhiko
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 79, 2015/03
So far, we clarified that X-ray irradiation induced cell killing by bystander effect mediated-secreted factor. This phenomenon was related with sphingomyelinase (SMase). In this study we analyzed mechanism of secreted SMase from irradiated cells after irradiation. SMase was detected in the culture medium after irradiation by SDS-PAGE. Then, SMase was detected in the exosome of culture medium, but not out of exosome after irradiation. This result indicates that SMase was secreted as exosome from the irradiated cells.
Watanabe, Kazuyo*; Akitsuki, Takashi*; Shimura, Sachiko*; Gusev, O.*; Cornette, R.*; Kikawada, Takahiro*; Sakashita, Tetsuya; Funayama, Tomoo; Kobayashi, Yasuhiko; Okuda, Takashi*
JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 87, 2015/03
The Sleeping Chironomid, can stand complete desiccation (anhydrobiosis) and also shows radio-resistance. Recently, we have generated cultured cell (Pv11) originated from embryo which can also stand complete dehydration. In this study, we examine the tolerance of cultured cell Pv11 against ionbeam irradiation.
Horikawa, Daiki*; Yamaguchi, Ayami*; Sakashita, Tetsuya; Tanaka, Daisuke*; Hamada, Nobuyuki*; Yukuhiro, Fumiko*; Kuwahara, Hirokazu*; Kunieda, Takekazu*; Watanabe, Masahiko*; Nakahara, Yuichi*; et al.
Astrobiology, 12(4), p.283 - 289, 2012/04
Times Cited Count:23 Percentile:68.44(Astronomy & Astrophysics)We examined the hatchability of hydrated and anhydrobiotic eggs of the tardigrade to hatch after ionizing irradiation (helium ions), extremely low and high temperatures, and high vacuum. Anhydrobiotic eggs (50% lethal dose; 1690 Gy) were substantially more radioresistant than hydrated ones (50% lethal dose; 509 Gy). Anhydrobiotic eggs also have a broader temperature resistance compared with hydrated ones. Over 70% of the anhydrobiotic eggs treated at high and low temperatures, but all of the hydrated eggs failed to hatch. After exposure to high vacuum conditions, the hatchability of the anhydrobiotic eggs was comparable to that of untreated control eggs.
Watanabe, Ritsuko; Wada, Seiichi*; Funayama, Tomoo; Kobayashi, Yasuhiko; Saito, Kimiaki; Furusawa, Yoshiya*
Radiation Protection Dosimetry, 143(2-4), p.186 - 190, 2011/02
Times Cited Count:16 Percentile:75.56(Environmental Sciences)Microscopic energy deposition pattern in an ion track is thought to affect on the spatial distribution of DNA damage as well as the damage spectrum. In this study, we focus on the intra-track spatial distribution of DNA damage in cellular condition based on the energy deposition pattern for each ion obtained by the detailed Monte Carlo track structure simulation. The estimation was performed for C and Ne ions with similar LET around 440 keV/m. As a result, radial DNA damage distribution shows different pattern for C and Ne ions. That is, DSBs or non-DSB type clustered damage are formed in the limited central area while the isolated damages as SSBs and base lesions are spread in larger area. Such tendency is more clearly shown for Ne ions than C ions. This result shows good agreement with the previously obtained experimental observation at TIARA, which indicates the different types of DNA damage shows different distribution pattern around C and Ne projectiles in cell nuclei.
Horikawa, Daiki*; Kunieda, Takekazu*; Abe, Wataru*; Watanabe, Masahiko*; Nakahara, Yuichi*; Yukuhiro, Fumiko*; Sakashita, Tetsuya; Hamada, Nobuyuki*; Wada, Seiichi*; Funayama, Tomoo; et al.
Astrobiology, 8(3), p.549 - 556, 2008/06
Times Cited Count:96 Percentile:91.30(Astronomy & Astrophysics)We report the successful rearing of the herbivorous tardigrade, , by supplying the green alga as food. The life span was 35 d, deposited eggs required 5.7 d to hatch, and animals began to deposit eggs 9 d after hatching. The reared individuals of this species had an anhydrobiotic capacity throughout their life cycle in egg, juvenile, and adult stages. Furthermore, the reared adults in an anhydrobiotic state were tolerant of temperatures of 90C and -196C and exposure to 99.8% acetonitrile or irradiation with 4000 Gy He ions. Based on their life history traits and tolerance to extreme stresses, might be a suitable model for astrobiological studies of multicellular organisms.
Nakahara, Yuichi*; Watanabe, Masahiko*; Kikawada, Takahiro*; Fujita, Akihiko*; Horikawa, Daiki*; Okuda, Takashi*; Sakashita, Tetsuya; Funayama, Tomoo; Hamada, Nobuyuki*; Wada, Seiichi*; et al.
JAEA-Review 2007-060, JAEA Takasaki Annual Report 2006, P. 113, 2008/03
We have shown that anhydrobiotic larvae of have higher tolerance against both high linear energy transfer (LET) radiation than hydrated larvae. We therefore examined effects of high-LET radiation on four kinds of larvae: (1) normal hydrated (intact) larva, (2) intermediates between the anhydrobiotic and normal hydrated state, (3) almost completely dehydrated (anhydrobiotic) larvae, and (4) immediately rehydrated larvae that are assumed to have a similar molecular profile to anhydrobiotic larvae. The intermediates and immediately rehydrated larvae survived longer after high-LET radiation than intact larvae, indicating that radiation tolerance could be enhanced even in hydrated larvae. Physiological changes toward anhydrobiosis, e.g. accumulation of protectants or increasing damage repair capacity, correlate with improved radiation tolerance in hydrated larvae.
Funayama, Tomoo; Wada, Seiichi*; Yokota, Yuichiro; Fukamoto, Kana; Sakashita, Tetsuya; Taguchi, Mitsumasa; Kakizaki, Takehiko*; Hamada, Nobuyuki*; Suzuki, Michiyo; Furusawa, Yoshiya*; et al.
Journal of Radiation Research, 49(1), p.71 - 82, 2008/01
Times Cited Count:47 Percentile:78.54(Biology)Research concerning cellular responses to low dose irradiation, radiation-induced bystander effects, and the biological track structure of charged particles has recently received particular attention in the field of radiation biology. Target irradiation employing a microbeam represents a useful means of advancing this research by obviating some of the disadvantages associated with the conventional irradiation strategies. The heavy-ion microbeam system at JAEA-Takasaki can provide target irradiation of heavy charged particles to biological material at atmospheric pressure using a minimum beam size 5 m in diameter. The system can be applied to the investigation of mechanisms within biological organisms not only in the context of radiation biology, but also in the fields of general biology such as physiology, developmental biology and neurobiology, and should help to establish and contribute to the field of "microbeam biology".
Horikawa, Daiki*; Sakashita, Tetsuya; Katagiri, Chihiro*; Watanabe, Masahiko*; Kikawada, Takahiro*; Nakahara, Yuichi*; Hamada, Nobuyuki*; Wada, Seiichi*; Funayama, Tomoo; Higashi, Seigo*; et al.
JAEA-Review 2006-042, JAEA Takasaki Annual Report 2005, P. 116, 2007/02
no abstracts in English
Watanabe, Masahiko*; Sakashita, Tetsuya; Fujita, Akihiko*; Kikawada, Takahiro*; Nakahara, Yuichi*; Hamada, Nobuyuki*; Horikawa, Daiki*; Wada, Seiichi*; Funayama, Tomoo; Kobayashi, Yasuhiko; et al.
International Journal of Radiation Biology, 82(12), p.835 - 842, 2006/12
Times Cited Count:19 Percentile:77.36(Biology)The aim of this study is to characterize the tolerance to high-LET radiations of. Larval survival and subsequent metamorphoses were compared between anhydrobiotic (dry) and non-anhydrobiotic (wet) samples after exposure to 1 to 7000 Gy of three types of heavy ions with LET values ranging from 16.2 to 321 keV/m. At all LET values measured, dry larvae consistently showed greater radiation tolerance than hydrated larvae, due to the disaccharide trehalose in anhydrobiotic animals. Relative biological effectiveness (RBE) values based on the median inhibitory doses reached a maximum at 116 keV/m (C). Anhydrobiosis potentiates radiation tolerance in terms of larval survival, pupation and adult emergence of exposed to high-LET radiations. larvae might have more efficient DNA damage repair after radiation than other chironomid species.
Horikawa, Daiki*; Sakashita, Tetsuya; Katagiri, Chihiro*; Watanabe, Masahiko*; Kikawada, Takahiro*; Nakahara, Yuichi*; Hamada, Nobuyuki*; Wada, Seiichi*; Funayama, Tomoo; Higashi, Seigo*; et al.
International Journal of Radiation Biology, 82(12), p.843 - 848, 2006/12
Times Cited Count:132 Percentile:99.15(Biology)no abstracts in English
Watanabe, Masahiko*; Sakashita, Tetsuya; Fujita, Akihiko*; Kikawada, Takahiro*; Horikawa, Daiki*; Nakahara, Yuichi*; Wada, Seiichi*; Funayama, Tomoo; Hamada, Nobuyuki*; Kobayashi, Yasuhiko; et al.
International Journal of Radiation Biology, 82(8), p.587 - 592, 2006/08
Times Cited Count:35 Percentile:89.90(Biology)The present study aims to evaluate effects of anhydrobiosis on radiation tolerance in an anhydrobiotic insect, Polypedilum vanderplanki. Larval survival (48 h), anhydrobiotic ability, metamorphosis and reproduction after exposure to 1 to 9000 Gy of -rays at the larval stage were comparedbetween anhydrobiotic (dry) and normal (wet) phases. Wet larvae were killed in a dose-dependent manner at doses higher than 2000 Gy, and all died within 8 h after 4000 Gy exposure. In contrast, dry larvae survived even 5000 Gy, and some of them still tolerated 7000 Gy and were alive at 48 h after rehydration. Moreover, greater radiotolerance of dry larva, compared to wet ones, was demonstrated interms of metamorphoses. However, anhydrobiosis did not protect against radiation damage in terms of producing viable offspring. These results indicate that anhydrobiosis enhances radiotolerance, resulting in increases of successful metamorphoses.
Ling, E.*; Shirai, Koji*; Kanekatsu, Rensuke*; Kiguchi, Kenji*; Kobayashi, Yasuhiko; Funayama, Tomoo; Watanabe, Hiroshi*
Developmental and Comparative Immunology, 30(6), p.531 - 543, 2006/00
Times Cited Count:13 Percentile:54.15(Fisheries)no abstracts in English
Watanabe, Tomoo; Ozawa, Kazumasa; Otsuka, Jiro; Sasaki, Kazuichi; Sawada, Makoto
JNC TN4440 2005-001, 40 Pages, 2005/07
no abstracts in English
Tanaka, Masashi*; Narumi, Issei; Funayama, Tomoo; Kikuchi, Masahiro; Watanabe, Hiroshi*; Matsunaga, Tsukasa*; Nikaido, Osamu*; Yamamoto, Kazuo*
Journal of Bacteriology, 187(11), p.3693 - 3697, 2005/06
Times Cited Count:47 Percentile:62.08(Microbiology)no abstracts in English
Funayama, Tomoo; Wada, Seiichi; Kobayashi, Yasuhiko; Watanabe, Hiroshi*
Radiation Research, 163(2), p.241 - 246, 2005/02
Times Cited Count:32 Percentile:64.67(Biology)As the first step for the analysis of the biological effect of heavy charged-particle radiation, we established a method for the irradiation of individual cells with a heavy ion microbeam apparatus at JAERI-Takasaki. CHO-K1 cells attached on the thin film of ion track detector, CR-39, were automatically detected under fluorescent microscope, and irradiated individually with Ar ion (11.5 MeV/u, LET=1260 keV/m) microbeam. Without killing the irradiated cells, trajectories of irradiated ions were visualized as etch-pits by treatment of CR-39 with alkaline-ethanol solution at 37C. The exact positions of ion hits were determined by overlaying images of both cells and etch-pits. The cells that were irradiated with argon ions showed a reduced growth in post-irradiate observations. Moreover, a single hit of an argon ion to the cell nucleus resulted in strong growth inhibition. These results tell us that our truthful irradiation method now enables us to start a precise study of the cellular response of high-LET radiation effects on cells.