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Journal Articles

Radiation biology of ${it Caenorhabditis elegans}$; Germ cell response, aging and behavior

Sakashita, Tetsuya; Takanami, Takako*; Yanase, Sumino*; Hamada, Nobuyuki*; Suzuki, Michiyo; Kimura, Takafumi*; Kobayashi, Yasuhiko; Ishii, Naoaki*; Higashitani, Atsushi*

Journal of Radiation Research, 51(2), p.107 - 121, 2010/03

https://doi.org/10.1269/jrr.09100
 Times Cited Count：41 Percentile：67.38(Biology)

The study of radiation effect in ${it Caenorhabditis (C.) elegans}$ have been carried out over three decades and now allow for understanding at the molecular, cellular and individual levels. This review describes the current knowledge of the biological effects of ionizing irradiation with a scope of the germ line, aging and behavior. ${it C. elegans}$ may be a good ${it in vivo}$ model system in the field of radiation biology.

Journal Articles

Effects of ionizing radiation on locomotory behavior and mechanosensation in ${it Caenorhabditis elegans}$

Suzuki, Michiyo; Sakashita, Tetsuya; Yanase, Sumino*; Kikuchi, Masahiro; Oba, Hirofumi; Higashitani, Atsushi*; Hamada, Nobuyuki*; Funayama, Tomoo; Fukamoto, Kana; Tsuji, Toshio*; et al.

Journal of Radiation Research, 50(2), p.119 - 125, 2009/04

https://doi.org/10.1269/jrr.08087
 Times Cited Count：8 Percentile：28.88(Biology)

Journal Articles

Locomotion-learning behavior relationship in ${it Caenorhabditis elegans}$ following $$gamma$$-ray irradiation

Sakashita, Tetsuya; Hamada, Nobuyuki*; Ikeda, Daisuke*; Suzuki, Michiyo; Yanase, Sumino*; Ishii, Naoaki*; Kobayashi, Yasuhiko

Journal of Radiation Research, 49(3), p.285 - 291, 2008/05

https://doi.org/10.1269/jrr.07102
 Times Cited Count：12 Percentile：38.83(Biology)

We investigated the effects of ionizing radiation (IR) exposure on the relationship between locomotion and salt chemotaxis learning behavior of ${it C. elegans}$. We found that effects of pre-learning irradiation on locomotion were significantly correlated with the salt chemotaxis learning performance, whereas locomotion was not directly related to chemotaxis to NaCl. On the other hand, locomotion was positively correlated with salt chemotaxis of animals which were irradiated during learning, and the correlation disappeared with increasing doses. These results suggest an indirect relationship between locomotion and salt chemotaxis learning in ${it C. elegans}$, and that IR inhibits the innate relationship between locomotion and chemotaxis, which is related to salt chemotaxis learning conditioning of ${it C. elegans}$.

Journal Articles

Modulatory effect of ionizing radiation on food-NaCl associative learning; The Role of $$gamma$$ subunit of G protein in ${it Caenorhabditis elegans}$

Sakashita, Tetsuya; Hamada, Nobuyuki*; Ikeda, Daisuke*; Yanase, Sumino*; Suzuki, Michiyo; Ishii, Naoaki*; Kobayashi, Yasuhiko

FASEB Journal, 22(3), p.713 - 720, 2008/03

https://doi.org/10.1096/fj.07-9259com
 Times Cited Count：11 Percentile：36.45(Biochemistry & Molecular Biology)

We analyzed the effects of ionizing radiation (IR) on a food-NaCl associative learning paradigm of adult ${it Caenorhabditis elegans}$, and observed that a decrease in chemotaxis toward NaCl occurs only after combined starvation and exposure to NaCl. Exposure to IR induced an additional decrease in chemotaxis immediately after an acute dose in the transition stage of the food-NaCl associative learning. Strikingly, chronic irradiation induced negative chemotaxis in the exposed animals, i.e., the primary avoidance response. IR-induced additional decreases in chemotaxis following acute and chronic irradiations were significantly suppressed in the ${it gpc-1}$ mutant, which was defective in GPC-1. Chemotaxis to cAMP, but not to lysine and benzaldehyde, was influenced by IR during the food-NaCl associative learning. Our novel findings suggest that IR behaves as a modulator in the food-NaCl associative learning via ${it C. elegans}$ GPC-1 and a specific neuronal network.

Journal Articles

Effects of $$gamma$$-ray irradiation on olfactory adaptation to benzaldehyde in ${it Caenorhabditis elegans}$

Sakashita, Tetsuya; Hamada, Nobuyuki*; Suzuki, Michiyo; Ikeda, Daisuke*; Yanase, Sumino*; Ishii, Naoaki*; Kobayashi, Yasuhiko

Uchu Seibutsu Kagaku, 21(4), p.117 - 120, 2007/12

https://doi.org/10.2187/bss.21.117

Using ${it Caenorhabditis elegans}$ as a model organism for studying the nervous system, we investigated the effects of $$gamma$$-ray irradiation on olfactory adaptation to benzaldehyde, in which animals show a decrease in chemotaxis to benzaldehyde. Irradiation during the conditioning for adaptation to benzaldehyde did not induce an additional decrease in chemotaxis immediately after irradiation. On the other hand, at 1 h after irradiation, the progress of adaptation to benzaldehyde was interrupted by irradiation with the highest dose. These results indicate that impaired olfactory adaptation to benzaldehyde following irradiation is different from salt chemotaxis learning in ${it C. elegans}$. Our findings suggest that the profile of radiation-induced response depends on the function of the nervous system in ${it C. elegans}$.

Oral presentation

Modulatory effect of ionizing radiation on food-NaCl associative learning; The Role of $$gamma$$ subunit of G protein in ${it Caenorhabditis elegans}$

Sakashita, Tetsuya; Hamada, Nobuyuki*; Ikeda, Daisuke*; Yanase, Sumino*; Suzuki, Michiyo; Ishii, Naoaki*; Kobayashi, Yasuhiko

no journal, , 

Exposure to IR induced an additional decrease in chemotaxis immediately after an acute dose in the transition stage of the salt chemotaxis learning. Chronic irradiation induced negative chemotaxis in the exposed animals, i.e., the primary avoidance response. IR-induced additional decreases in chemotaxis following acute and chronic irradiations were significantly suppressed in the ${it gpc-1}$ mutant, which was defective in GPC-1 (one of the two $$gamma$$ subunits of the heterotrimeric G-protein). Chemotaxis to cAMP, but not to lysine and benzaldehyde, was influenced by IR during the food-NaCl associative learning. Our novel findings suggest that IR behaves as a modulator in the salt chemotaxis learning via ${it C. elegans}$ GPC-1 and a specific neuronal network, and may shed light on the modulatory effect of IR on learning.

Oral presentation

The Video-based quantitative evaluation of IR-induced effects on locomotory behavior in ${it Caenorhabditis elegans}$

Suzuki, Michiyo; Sakashita, Tetsuya; Hattori, Yuya; Yanase, Sumino*; Kikuchi, Masahiro; Funayama, Tomoo; Yokota, Yuichiro; Tsuji, Toshio*; Kobayashi, Yasuhiko

no journal, , 

We reported an IR-induced reduction of locomotory rate of ${it Caenorhabditis elegans}$ in the absence of food. In the previous experiments, wild-type animals were irradiated with $$gamma$$-rays in the whole body, and measured the motility using "body bends" (the number of bends in the anterior body region at 20-s intervals). However, the IR-induced effects in the central and posterior body region were not evaluated by the body bends. In the present study, to investigate the IR-induced effects in more detail, we propose a novel method to evaluate the motility of the whole body using the video-based analysis.

Oral presentation

Detection and measurement of abnormal posture under stressful conditions in nematode $textit{C. elegans}$

Ide, Kazunori*; Ishikawa, Tomoya*; Arai, Shingo*; Morioka, Tatsuya*; Kaneda, Hiroki*; Suzuki, Michiyo; Sakashita, Tetsuya; Ishii, Naoaki*; Yanase, Sumino*

no journal, , 

Oral presentation

Approach to the radiobiological study of ${it Caenorhabditis elegans}$ using heavy-ion microbeam

Sakashita, Tetsuya; Suzuki, Michiyo; Hamada, Nobuyuki*; Ikeda, Daisuke*; Fukamoto, Kana; Yokota, Yuichiro; Funayama, Tomoo; Yanase, Sumino*; Higashitani, Atsushi*; Ishii, Naoaki*; et al.

no journal, , 

Using ${it Caenorhabditis elegans}$ as a model multicellular organism, we push forward a heavy-ion microbeam irradiation individual-study. As for about 1.2 mm in a range of carbon ions in water, all cells and tissues of ${it C. elegans}$ are microbeam irradiation objects. Sugimoto et al. showed DNA-damage-induced cell cycle arrest and apoptosis in locally irradiated areas of ${it C. elegans}$. We focus the nervous system of ${it C. elegans}$ and study the effects of localized irradiation on salt chemotaxis learning behavior. However, the anesthetic method for fixation of animals is not usable because the whole body irradiation of $$^{60}$$Co $$gamma$$ rays affected only transition stage of the conditioning for salt chemotaxis learning. Thus, now we are constructing the heavy-ion microbeam irradiation system for living target ${it C. elegans}$.

Oral presentation

Learning behavior of ${it C. elegans}$ and radiation effects

Sakashita, Tetsuya; Suzuki, Michiyo; Hamada, Nobuyuki*; Ikeda, Daisuke*; Yanase, Sumino*; Ishii, Naoaki*; Kobayashi, Yasuhiko

no journal, , 

We studied the effects of $$gamma$$-irradiation on the salt chemotaxis learning of ${it C. elegans}$, that is well known as a model organism for the nervous system. As a result, we found that chemotaxis to NaCl immediately after irradiation was significantly decreased only during the salt chemotaxis learning. In addition, the radiation-induced resoponse is significantly suppressed in the ${it gpc-1}$ mutant. These results suggested that the effects of ionizing radiation on the salt chemotaxis learning behavior of ${it C. elegans}$ mediated through GPC-1 localized in the specific sensory neurons.

Oral presentation

Modulatory effect of ionizing radiation on salt chemotaxis learning; The Role of G protein $$gamma$$ subunit in ${it Caenorhabditis elegans}$

Hamada, Nobuyuki*; Sakashita, Tetsuya; Ikeda, Daisuke*; Yanase, Sumino*; Suzuki, Michiyo; Ishii, Naoaki*; Kobayashi, Yasuhiko

no journal, , 

Well-fed adult animals were exposed to cobalt-60 $$gamma$$-rays, followed by the chemotaxis assay. A decrease in chemotaxis toward NaCl occurred only after combined starvation and exposure to NaCl. Irradiation led to an additional decrease in chemotaxis immediately after an acute dose in the transition stage of salt chemotaxis learning. Strikingly, chronic irradiation induced negative chemotaxis in the exposed animals, i.e., the primary avoidance response. Radiation-induced additional decreases in chemotaxis following acute and chronic irradiations were significantly suppressed in the ${it gpc-1}$ mutant, which was defective in GPC-1 (one of the two $$gamma$$ subunits of the heterotrimeric G protein). The data suggest that radiation behaves as a modulator in salt chemotaxis learning via ${it C. elegans}$ GPC-1 and a specific neuronal network, and may shed light on the modulatory effect of radiation on learning.

Oral presentation

Effects of $$gamma$$-ray irradiation on olfactory adaptation to benzaldehyde in ${it Caenorhabditis elegans}$

Sakashita, Tetsuya; Suzuki, Michiyo; Hamada, Nobuyuki*; Ikeda, Daisuke*; Yanase, Sumino*; Ishii, Naoaki*; Kobayashi, Yasuhiko

no journal, , 

Using ${it Caenorhabditis elegans}$ as a model organism for studying the nervous system, we investigated the effects of $$gamma$$-ray irradiation on olfactory adaptation to benzaldehyde, in which animals show a decrease in chemotaxis to benzaldehyde. Irradiation during the conditioning for adaptation to benzaldehyde did not induce an additional decrease in chemotaxis immediately after irradiation. On the other hand, at 1 h after irradiation, the progress of adaptation to benzaldehyde was interrupted by $$gamma$$-irradiation with the highest dose. These results indicate that impaired olfactory adaptation to benzaldehyde following $$gamma$$-irradiation is different from salt chemotaxis learning in ${it C. elegans}$. Our findings suggest that the profile of radiation-induced response depends on the function of the nervous system in ${it C. elegans}$.

Oral presentation

Detection and measurement of abnormal posture under stressful conditions in age-related mutants

Matsuda, Sakiyo*; Nitta, Nazuki*; Tamura, Miku*; Suzuki, Michiyo; Sakashita, Tetsuya; Ishii, Naoaki*; Yanase, Sumino*

no journal, , 

13 (Records 1-13 displayed on this page)
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