Tolerance of anhydrobiotic eggs of the tardigrade to extreme environments

Horikawa, Daiki*; Yamaguchi, Ayami*; Sakashita, Tetsuya; Tanaka, Daisuke*; Hamada, Nobuyuki*; Yukuhiro, Fumiko*; Kuwahara, Hirokazu*; Kunieda, Takekazu*; Watanabe, Masahiko*; Nakahara, Yuichi*; et al.

Astrobiology, 12(4), p.283 - 289, 2012/04

https://doi.org/10.1089/ast.2011.0669

We examined the hatchability of hydrated and anhydrobiotic eggs of the tardigrade to hatch after ionizing irradiation (helium ions), extremely low and high temperatures, and high vacuum. Anhydrobiotic eggs (50% lethal dose; 1690 Gy) were substantially more radioresistant than hydrated ones (50% lethal dose; 509 Gy). Anhydrobiotic eggs also have a broader temperature resistance compared with hydrated ones. Over 70% of the anhydrobiotic eggs treated at high and low temperatures, but all of the hydrated eggs failed to hatch. After exposure to high vacuum conditions, the hatchability of the anhydrobiotic eggs was comparable to that of untreated control eggs.

sp. nov., isolated from the high atmosphere

Yang, Y.*; Ito, Takashi*; Yokobori, Shinichi*; Itahashi, Shiho*; Shimada, Haruo*; Sato, Katsuya; Oba, Hirofumi; Narumi, Issei; Yamagishi, Akihiko*

International Journal of Systematic and Evolutionary Microbiology, 59, p.1862 - 1866, 2009/00

https://doi.org/10.1099/ijs.0.007963-0

An orange pigmented, non-motile, coccoid bacterial strain, TR0125, was isolated from dust samples collected in the high atmosphere above Japan. Phylogenetic analysis based on 16S rRNA gene sequences showed that it was within the radiation of species. Major peptidoglycan amino acids were D-glutamic acid, glycine, D-alanine, L-alanine and ornithine. Predominant fatty acids were 17:0 iso, iso 17:1 9c and 15:0 iso. Strong resistance to desiccation, UV-C and -radiation, high DNA G+C content also supported the affiliation of strain TR0125 to the genus . TR0125 had a highest similarity value (95.7 %) of the 16S rRNA gene sequence to the type strain of the species , and phylogenetic analysis shows that it was further separated from than from , indicating that strain TR0125 was not a member of these two species. Besides, there were phenotypic differences between strain TR0125 and type strains of the two species. Therefore, we propose a new species of the genus , sp. nov. (type strain, TR0125 = JCM 11750 = DSM 21212), to accommodate this isolate.

Discussion on possibility of "Search of Life" mission on Moon

Yokobori, Shinichi*; Haruyama, Junichi*; Yano, Hajime*; Narumi, Issei; Mita, Hajime*; Takahashi, Junichi*

no journal, , 

no abstracts in English

Investigation of the survivability of spp. under space

Kawaguchi, Yuko*; Yang, Y.*; Murano, Yuka*; Harada, Miyu*; Kawashiri, Narutoshi*; Shiraishi, Keisuke*; Takasu, Masako*; Narumi, Issey*; Sato, Katsuya; Hashimoto, Hirofumi*; et al.

no journal, , 

no abstracts in English

Microbe space exposure experiments at International Space Station (ISS) in the mission "Tanpopo"

Yokobori, Shinichi*; Yang, Y.*; Sugino, Tomohiro*; Kawaguchi, Yuko*; Takahashi, Yuta*; Narumi, Issei; Hashimoto, Hirofumi*; Hayashi, Nobuhiro*; Imai, Eiichi*; Kawai, Hideyuki*; et al.

no journal, , 

Alteration of organic compounds of interstellar origin in the solar system; Simulation experiments on the ground and in space

Kobayashi, Kensei*; Kawamoto, Yukinori*; Sarker, P. K.*; Ono, Keisuke*; Kuwahara, Hideharu*; Obayashi, Yumiko*; Kaneko, Takeo*; Mita, Hajime*; Yabuta, Hikaru*; Yoshida, Satoshi*; et al.

no journal, , 

Tanpopo project; Survival of microbes in outer space

Yokobori, Shinichi*; Kawaguchi, Yuko*; Yang, Y.*; Kawashiri, Narutoshi*; Shiraishi, Keisuke*; Shimizu, Yasuyuki*; Takahashi, Yuta*; Sugino, Tomohiro*; Narumi, Issei; Sato, Katsuya; et al.

no journal, , 

no abstracts in English

Tanpopo Working Group report; Space exposure of microbes and organic materials

Yokobori, Shinichi*; Kobayashi, Kensei*; Mita, Hajime*; Yabuta, Hikaru*; Nakagawa, Kazumichi*; Narumi, Issei; Hayashi, Nobuhiro*; Tomita, Kaori*; Kawaguchi, Yuko*; Shimizu, Yasuyuki*; et al.

no journal, , 

no abstracts in English

Current status of preparation of TANPOPO mission (astrobiology exposure and micrometeoroid capture experiments) and investigation of survivability of microbes in space

Yokobori, Shinichi*; Kawaguchi, Yuko*; Shimizu, Yasuyuki*; Kawashiri, Narutoshi*; Shiraishi, Keisuke*; Sugino, Tomohiro*; Takahashi, Yuta*; Yang, Y.*; Tanigawa, Yoshiaki*; Hashimoto, Hirofumi*; et al.

no journal, , 

no abstracts in English

Microbes capture and expose experiments on ISS proposed in Tanpopo mission

Kawaguchi, Yuko*; Sugino, Tomohiro*; Yang, Y.*; Takahashi, Yuta*; Yoshimura, Yoshitaka*; Tsuji, Takashi*; Kobayashi, Kensei*; Tabata, Makoto*; Hashimoto, Hirofumi*; Narumi, Issei; et al.

no journal, , 

no abstracts in English