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論文

Route and regulation of zinc, cadmium, and iron transport in rice plants (${it Oryza sativa}$ L.) during vegetative growth and grain filling; Metal transporters, metal speciation, grain Cd reduction and Zn and Fe biofortification

米山 忠克*; 石川 覚*; 藤巻 秀

International Journal of Molecular Sciences (Internet), 16(8), p.19111 - 19129, 2015/08

AA2015-0336.pdf:1.94MB

https://doi.org/10.3390/ijms160819111
 被引用回数：103 パーセンタイル：82.58(Biochemistry & Molecular Biology)

Zinc (Zn), iron (Fe) concentrations in rice grains harvested under different levels in soil are known to change only within a small range, while cadmium (Cd) concentrations show greater changes. To clarify the mechanisms underlying such difference, we synthesized information on the routes of metal transport and accumulation in rice plants. At grain-filling, Zn and Cd ascending in xylem are transferred to the phloem at stem nodes. Grain Fe is largely derived from the leaves by remobilization. Zn and Fe concentrations in phloem-sap and grains are regulated within a small range, while Cd concentrations vary depending on xylem supply. To increase concentrations of the metal chelators are effective in increasing grain Zn and Fe concentrations. The elimination of OsNRAMP5 Cd-uptake transporter and the enhancement of root-cell vacuolar Cd sequestration reduce uptake and root-to-shoot transport, respectively, resulting in a reduction of grain Cd accumulation.

論文

A Quick incorporation of $$^{13}$$N into the soluble high-molecular compound in rice (${it Oryza sativa}$ L.) roots by application of $$^{13}$$N-labeled nitrate/nitrite

米山 忠克*; 鈴井 伸郎; 石岡 典子; 藤巻 秀

Soil Science and Plant Nutrition, 57(2), p.279 - 282, 2011/05

https://doi.org/10.1080/00380768.2011.576399
 被引用回数：4 パーセンタイル：14.11(Plant Sciences)

The nitrate/nitrite response, which occurs within minutes for the gene expression of the primary nitrogen metabolism in higher-plant roots, has almost been described, but the intermediate signaling molecule that exerts in signal transduction remains to be elucidated. We hypothesized that the constitutively present signaling molecule may bind quickly to exogenously applied nitrate/nitrite to high-molecular compounds, which may include intermediate signaling molecules, in the roots of the rice plant (${it Oryza sativa}$ L.), the segmented roots of rice seedlings were exposed to $$^{13}$$N-labeled nitrate/nitrite for either 2 or 5 min at 25$$^{circ}$$C. The supernatant of the Tris-buffer root extract was separated by a desalting pre-packed column. The incorporation of $$^{13}$$N into the high-molecular fraction was identified by $$^{13}$$N feeding for 2 min, and by $$^{13}$$N feeding for 5 min, however, the amount of $$^{13}$$N incorporation was not increased. Although our findings are preliminary, it was clearly demonstrated that $$^{13}$$N-labeled nitrate/nitrite was quickly incorporated into a limited amount of the soluble high-molecular compound(s). The formation of NO$$_{x}$$-bound proteins, which may play a role in nitrate/nitrite signal transduction, is discussed.

口頭

$$^{13}$$NO$$_{3}$$$$^{-}$$ ($$^{13}$$NO$$_{2}$$$$^{-}$$)は短時間でイネ幼植物の根の高分子画分へ結合する

米山 忠克*; 鈴井 伸郎; 石岡 典子; 藤巻 秀

no journal, , 

植物の根において硝酸を吸収・代謝するタンパク質のmRNAの発現は、硝酸投与後20-40分で始まる。これらのmRNAの生成はNO$$_{3}$$$$^{-}$$特異的であり、発現を制御する転写因子の生成には新しいタンパク質の合成を必要としないことから、硝酸(亜硝酸)が短時間に何らかの物質に結合し、それが硝酸シグナリングの「中間物」となり、これらのmRNA合成を誘導すると考えられる。そこで本研究では、この「中間物」の存在とその性質を明らかにすることを目的とした。高比活性の$$^{13}$$NO$$_{3}$$$$^{-} $$ ($$^{13}$$NO$$_{2}$$$$^{-}$$)トレーサーを20日令イネの切断根端に数分間投与した後、リン酸バッファーで抽出、HisTrap脱塩カラムで分画したところ、高分子画分に$$^{13}$$Nが検出された。この結果から、植物が硝酸を感知する際に高分子の物質が関与することが示唆された。

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